ABSTRACT
Abstracts: Background: Wound infections are one of the most common hospital acquired infections and are an important cause of morbidity and account for 70-80% mortality. Pseudomonas aeruginosa is an epitome of opportunistic nosocomial pathogen & responsible for serious infection such as septicemia ,pneumonia ,various pyogenic & wound infections. Pseudomonas aeruginosa is inherently resistant to many antibiotics and can mutate to even more resistant strains during therapy. So the present study aimed to find out the strains of Pseudomonas aeruginosa from various pyogenic & wound infections, their antibiotic sensitivity profile & to find out multidrug resistant strains. Methodology: Pseudomonas aeruginosa isolates obtained from pyogenic & wound infection samples were identified by conventional microbiological techniques. All these isolates were tested for antimicrobial susceptibility on Muller-Hinton’s agar by Kirby-Bauer disk diffusion method as per CLSI guidelines. Results: Out of 90 Pseudomonas aeruginosa strains,49 (54.44 %) were MDR strains & highest sensitivity was found to levofloxacin (74.44 %) ,amikacin, (67.77%),cefepime(65.55 %), pipercillin (64.4%) & ceftazidime (63.33 %). Conclusion: The prevalence of MDR strains in our study is 54.4 % which calls for the judicious selection of antibiotics in clinical practice. In addition, regular antimicrobial susceptibility surveillance is essential for area-wise monitoring of the resistance patterns. An effective national and state level antibiotic policy and draft guidelines should be introduced to preserve the effectiveness of antibiotics and for better patient management.
ABSTRACT
Out of hundred syringes and hundred needles (both presterile, disposable) tested, 41 syringes (41%) and 6 needles (6%) showed aerobic growth. Forty of the above syringes were tested simultaneously for fungi and anaerobes. Fungi isolated were 5.7% and no anaerobe was grown. Fifty glass syringes and fifty needles autoclaved in the departmental laboratory served as controls and did not show any growth. As a preventive measure, proper disposal of used disposable material should be made mandatory so that it does not find its way into the market. Or has the time come to switch back to the former conventional practice of using in house autoclaved articles?