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1.
Article | IMSEAR | ID: sea-126862

ABSTRACT

Stool specimens from 200 cases of children with ages of less than five years with history of acute diarrhoea from Yangon Children's Hospital were proceeded for isolation of bacterial pathogens. Enterotoxigenic Escherichia coli ETEC (15.5 per cent), enteropathogenic Escherichia coli EPEC (19 per cent), Shigellae (3.5 per cent), Salmonellae (2.5 per cent), ETEC + EPEC (6.5 per cent), ETEC+Shigellae (1.0 per cent) were isolated. Antibiotic susceptibility pattern of EPEC revealed that they were resistant to ampicillin (96 per cent), tetracycline (90 per cent), streptomycin (82 per cent), chloramphenicol (80 per cent) and septrin (61 per cent); but were sensitive to nalidixic acid (100 per cent), furazolidone (92 per cent), sisomycin (90 per cent), gentamicin (88 per cent), norfloxacin (70 per cent), and cephalothin (66 per cent). For Shigellae they were resistant to ampicillin (100 per cent), cephalothin, chloramphenicol, furazolidone, streptomycin (66.7 per cent) but were 100


sensitive to sisomycin, minocycline, norfloxacin, gentamicin; tobramycin (89 per cent), neomycin (67 per cent) and septrin (56 per cent). For Salmonellae they were resistant to ampicillin (100 per cent), chloramphenicol and tetracycline (80 per cent) but were 100 per cent sensitive to cephalothin, furazolidone, gentamicin, nalidixic acid, norfloxacin, septrin and sisomycin. For ETEC they were resistant to ampicillin (100 per cent), tetracycline (79 per cent), chloramphenicol (53 per cent) and septrin (53 per cent ). They were 100 per cent sensitive to cephalothin, furazolidone, gentamicin, nalidixic acid, neomycin, norfloxacin, septrin and sisomycin.


Subject(s)
Anthropometry , Diet , Diet, Vegetarian , Eating , Myanmar
2.
Southeast Asian J Trop Med Public Health ; 1995 Dec; 26(4): 664-8
Article in English | IMSEAR | ID: sea-35261

ABSTRACT

Genotype of three dengue-2 virus strains from Myanmar was determined as genotype II by sequencing 240 nucleotide long fragment across the E/NS1 gene junction by the primer extension dideoxy chain termination method, applying direct sequencing of the PCR product. These strains were isolated from a dengue shock syndrome (DSS) patient and two patients with dengue hemorrhagic fever (DHF) grade 1, in Yangon (Rangoon), Myanmar (Burma), in 1987. Sequence homology of all three strains were highest (96%) to New Guinea C strain (genotype II), lesser homology (93%) to Jamaican 1409 strain (genotype III), and the least homology (91%) to PR 159/S1 strain (genotype I). Two DHF strains revealed only 2 nucleotide and 3 nucleotide differences compared with DSS strain, all at the 3rd position of the codons which resulted in silent mutations.


Subject(s)
Amino Acid Sequence , Base Sequence , DNA, Viral/analysis , Dengue/epidemiology , Dengue Virus/classification , Genotype , Humans , Molecular Sequence Data , Myanmar/epidemiology , Sequence Alignment , Sequence Homology, Nucleic Acid , Viral Envelope Proteins/genetics , Viral Nonstructural Proteins/genetics
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