Hydrogen oxidizing bacteria as poly [hydroxybutyrate] producers

Batch culture of Ralstonia eutropha using the recycled gas closed circuit culture system was conducted in order to develop a practical fermentation system for industrial autotrophic culture for poly [hydroxybutrate] production. The gas phase of the culture system consisted of substrate gas so that gases in this culture could be recycled as long as the amount of the gas consumed would be replenished. All gases supplied into this system could be completely used without any loss as exhaust. Studies on the effect of oxygen concentration showed that high concentration of this gas suppressed the specific growth rate while a low oxygen concentration promoted it