ABSTRACT
Background:A good number of medicinal and dietary plants are used for diabetes treatment in Burkina Faso. Aim of the Study:The present study aimed to investigate the antidiabetic activity of Guiera senegalensisgalls extracts and its potential mechanisms in streptozotocin-induced diabetic rats. Methodology:The methanol extract was administered by gavage to healthy Wistar rats for the determination of toxicity, to normal and diabetic Wistar rats forthe determination of glucose reduction level, lipid profile, insulin level and glycaemic parameters in serum. The histology and immunohistochemistry of thepancreas were also determined.Results:The acute toxicity results showed that the medium lethal dose (LD50) of the methanol galls extract of Guiera senegalensisis greater than 2000 mg/kg body weight in rats. Guiera senegalensismethanolic extract (250 mg/kg) and the tolbutamide (100 mg/kg) recorded a significantly (p < 0.05)lower level of triglyceride compared to the diabetic group. The methanol extract (250 and 500 mg/kg pc) significantly (p < 0.05)decreased the blood glucose level and increased the serum insulin level in diabetic rats. Interestingly, improved ß-cell function and antioxidant status were also observed in G. senegalensis-treated diabetic rats when compared to tolbutamide-treated diabetic rats.Conclusion:These data showed direct evidence that G. senegalensishas antidiabetic activity by decreasing blood glucose level, improving insulin secretion and β-cell functions and modulating antioxidant status
ABSTRACT
Aims: To assess the relevance of Chrysanthellum americanum(L.) Vatke extracts in rat liver protection. Place and Duration of Study:Laboratory of Biochemistry and Applied Chemistry (LABIOCA), also in Laboratory of Department of Medicine and Traditional Pharmacopoeia (MEPHATRA-PH) of Institute for Research in Health Sciences (IRSS/CNRST) of Burkina Faso between July 2014 and August 2015.Study Design:Polyphenolic extract of Chrysanthellum americanum-in vivo liver protection-in vivoliver intoxication-liver necrosis parameters analysis, histopathology analysis, in vivoand in vitroantioxidant assay. Background:Chrysanthellum americanumL. (Vatke) is a medicinal plant well known for its flavonoids and saponins richness, but also for its strong antioxidant potential and use traditionally for liver disease treatment.Methodology:In vivo, anti hepatotoxicity effects of Chrysanthellum americanumwas evaluated using CCl4as hepatotoxic agent. Also, acute toxicities were determined using standards methods, serum parameters of liver injury using Cypress Diagnostics kits and histopathology analysis using Mayer's haematoxylin-eosin-phloxine coloration method. For in vitro tests, malondialdehyde and thiobarbituric acid method were used in lipid peroxidation assessment and the ABTS method in Trolox Equivalent Antioxidant Capacity assessing.Results:Result showed that the crude extract of C. americericumhas a very low oral toxicity but, in intraperitoneal route this extract presented a high toxicity (LD50= 175 mg / kg of body weight). Histopathology micrograph indicated reduction in number of necrotic cells induced by CCl4. This beneficial action was confirmed by reduction in serum transaminases and malondialdehyde (22.68 ± 0.68 mmol MDA/ g of liver weight). In vitroantioxidant capacities, this plant extract presented a result of 35.01 ± 0.26 % and 42.01 ± 0.26 mg TE/ g respectively in LPO and TEAC.Conclusion:Given our results, our research confirms that Chrysanthellum americanumextracts have in vivophysiological impact and benefits in traditional medicine for specific care of liver diseases.
ABSTRACT
Guiera senegalensis is a well known medicinal plant which is used as a drug in Burkina Faso. The aim of this study was to investigate the chemical composition and antifungal activity from galls of Guiera senegalensis against different kinds of fungi in vitro. The chemical composition of the Guiera senegalensis volatile compounds obtained from the galls was analysed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Thirteen (13) components were identified for hexane-acetone (50:50) column fraction of hydroacetone extract and twenty one (21) compounds for hexane-acetone (50:50) column fraction of aqueous decoction extract. This composition differed according to the kind of extract. The ethyl acetate fraction extract from hydroacetone extract (EAF/HAE) exhibited the highest of flavonol content (0.56 ± 0.01 mg QE/100 mg of fraction). The G senegalensis exhibited an interesting antifungal activity against all strains tested.
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In this investigation, we evaluated essential oils from six medicinal plants from Burkina Faso for their antiacetylcholinesterase and antioxidant abilities. The chemotype of most active were also determined. The best antiacetylcholinesterase activities were recorded for the essential oils of Eucalyptus camaldulensis (IC50 18.98 µ g/mL) and Ocimum canum (IC50 36.16 µ g/mL). Their chemotype have been related to the 1,8-cineole one. Both essential oils demonstrated a linear mixed non competitive inhibition. The essential oil of Ocimum basilicum which belong to the linalool-eugenol chemotype exhibited the best radical scavenging activity (IC50 3.82 µ g/mL) and reducing power (531.75 mg AAE/g). In comparison with gallic and ascorbic acids, O. basilicum essential oil evidenced interesting antioxidant activities. The antiacetylcholinesterase and antioxidant activities of essential oils were discussed in regard with their chemical composition.