Anti-inflammatory Constituents from Branches of Corylus hallaisanensis Nakai.

Development of bioactive ingredients from natural sources has long been the research project of our laboratory. In this study, the extract from Corylus hallaisanensis Nakai branches was investigated and their anti-inflammatory constituents were identified. The prepared ethanol extract was successively partitioned into n-hexane, ethyl acetate, n-butanol and aqueous layers. Upon anti-inflammatory screenings, ethyl acetate fraction exhibited good nitric oxide production inhibitory activity in lipopolysaccharide-induced RAW 264.7 cells. Further phytochemical studies for the ethyl acetate fractions led to isolation of four constituents such as β-sitosterol (1), 3,3’,4’-tri-O-methylellagic acid (2), carpinontriol A (3) and carpinontriol B (4). All of the compounds were isolated for the first time from this plant. The isolates 2, 3 and 4 showed considerable inhibition on the production of nitric oxide in the RAW 264.7 cell without causing cell toxicities. And compounds 3 and 4 reduced the production of interleukin-6, an inflammatory cytokine, in dose-dependent manner in RAW 264.7 cells. Based on these results, C. hallaisanensis extracts could be potentially applicable as anti-inflammatory agents in pharmaceutical or cosmetic industries.

Constituents from the Branches of Sambucus sieboldiana var. pendula with the Properties of Collagen Synthesis Activation.

For the purpose of developing anti-wrinkle cosmetic ingredients, the extracts from branches of a woody plant Sambucus sieboldiana var. pendula were examined on collagen synthesis activities using fibroblast HDFn cells. As a result, the S. sieboldiana ethanol extract (SSE) proved to activate the production of type I procollagen in a dose-dependent manner without showing cell toxicity. Phytochemical study was conducted to isolate the active constituents in the extracts by solvent fractionation followed by chromatographic purifications. From this procedure, two known compounds, kaempferol 3-O-sophoroside (1) and daucosterol (2), were identified by spectroscopic studies. From the isolates, the flavonoid glycoside 1 was verified to induce the synthesis of the type I procollagen dose-dependently. These results suggested that S. sieboldiana extract containing the flavonoid 1 could be useful as an active ingredient in wrinkle-care cosmetics.