ABSTRACT
DOT ELISA was compared with RT-PCR and tissue culture to detect RSV from nasopharyngeal aspirates. DOT ELISA had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. The results indicate that DOT ELISA can be used for screening detection of RSV from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries.
Subject(s)
Cells, Cultured , Child , Enzyme-Linked Immunosorbent Assay/methods , Humans , Infant , Polymerase Chain Reaction/methods , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Viruses/growth & development , Sensitivity and SpecificitySubject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Rural Health , Serologic Tests , Thailand , Toxoplasmosis/epidemiologyABSTRACT
Gnathostoma malaysiae Miyazaki and Dunn, 1965 was found in the stomach wall of Rattus surifer, captured in Phuket Island and Khao Yai National Park of Thailand. This is the first to be recorded in Thailand and the second discovery after the first description of Miyazaki and Dunn (1965). Pathological findings of infected animals were also described. Some new morphological descriptions were added to the original.
Subject(s)
Animals , Female , Gnathostoma/anatomy & histology , Liver/parasitology , Male , Muridae/parasitology , Nematode Infections/parasitology , Rats , Stomach/parasitology , Thailand , Thelazioidea/isolation & purificationABSTRACT
A serological survey was conducted in the villages of Phichit Province, Northern region and in the urban slum communities in Bangkok to determine whether amoebiasis was endemic in the areas and to determine the prevalence rates. Six rural villages, ethnically and culturally alike with a population of 3,019 and two urban slums with a population of 1,510 were surveyed. Sera were tested for indirect hemagglutination antibody (IHA) to Entamoeba histolytica and the stools examined for the parasite by direct smear method. Positive IHA titres (greater than or equal to 1:128) were detected in 482 (11%) and 176 (20%) sera, and E. histolytica found in 639 (2%) and 208 (3%) stool specimens of rural and urban slum populations respectively. Out of a total of 88 persons who showed significant levels of IHA antibodies to E. histolytica antigen, 5 had E. histolytica cysts in their stool specimens. The survey confirmed, serologically and parasitologically, that amoebiasis is endemic in the lower socio-economic areas. Substandard living and sanitary conditions within the areas were considered responsible for the transmission of the disease.