ABSTRACT
The purpose of this study was to evaluate "the effect of intensive activity and regular exercise with carbohydrate ingestion on cortizol, lymphocyte, monocyte, white blood cell, fast blood suger, CD3, CD4, CD8 and CD4 to CD8 ratio, cell-mediated immunity". This semi experimental, cross sectional and double blind study performed to investigate the responses of cell-mediated immunity after 12 weeks intensive activity and regular exercises. 39 sedentary and healthy students with same-life style, were selected. They did not use cigarette, alcohol, supplementary and they had not infection diseases. Students selected randomly and then divided into three groups: Glucose and train [TG] 23 +/- 2.1 years, 73 +/- 8.2 Kg, 179 +/- 4.7Cmand with 22 +/- 2.5 BMI. Train [T], 23.5 +/- 1.8 years, 71.7 +/- 7.8 Kg, 176.4 +/- 3.6 Cmand 22 +/- 1.9 BMI. Control [C] 23.7 +/- 1.1 years, 72.4 +/- 6.6 Kg, 174.8 +/- 3.5 Cmand 22 +/- 2 BMI, they did endurance and speed train for 12 weeks. TG group drank 2cc/Kg, glucose 5% monohydrate and water solution, in the middle of their train session. T group drank 150 - 200 ml water. Bloods sample were withdrawn from antecubital vein after 14 +/- 2/hr fasting and evaluated before, immediately and after 48/hr of train. As well as cellular determined by immunology assay. For analyze in between groups, one way ANOVA with significant used from modify method of green house-ghezer [GG], p=0.05 level, were used. There were not any differences in T, TG and C group before 12 weeks exercise. The amounts of cortizol in TG and T groups significantly increased after exercise, but there was a lower increase in TG group. Results was significantly decreased after 48/hrs of train in before and after train parameters. The FBS in TG and T groups had not any differences but in TG group / T group results was significantly lower, after 48 hours. There was not any diffrences in amount of LYM after train but after 48/hrs, it significantly increased in TG group. The amount of WBC increased in after train but significantly decreased after 48/hr in TG group. The CD4 significantly increased after train in TG group, and CD3 significantly increased after train and after 48/hrs in TG group. Monocytes, ratio CD4/CD8 and CD8 amount had not any differences in TG and T groups. This research showed that intensive activity and regular exercise with cabohydrat digestion induced increase some of immune cells and cellular defence against infection disease that caused from intensive exercises. Another finding of this investigation indicates that drinking a CHO solution during exercise, improves performance. This study has practical implications for those sports and drinking CHO solution during activity. Carbohydrates as a factor can increase security against infection disease risk in recovery time and after intensive exercise
Subject(s)
Humans , Motor Activity , Exercise , Carbohydrates , Eating , Cross-Sectional Studies , Double-Blind Method , Dietary CarbohydratesABSTRACT
Natural killer [NK] cells are the effector cells of innate immunity that respond to infection and tumor. Interactions between killer cell immunoglobulin like receptors [KIR] and human leukocyte antigen [HLA] class I molecules regulate NK cells responses to eliminate infected and transformed cells. To investigate the impact of KIR genes, HLA ligand genes, and KIR-HLA combinations on susceptibility to tuberculosis [TB] in Lur population of Iran. The genomic DNA of 50 patients with TB from Lorestan province of Iran was genotyped for sixteen KIR genes and their five major HLA class I ligands were determined by a polymerase chain reaction-sequence-specific primers [PCR-SSP] assay. The results were compared with those of 200 healthy unrelated Iranian individuals. In Lur population of Iran, a significant decrease in frequency of KIR3DS1 was found in TB patients compared to control group [24% vs. 44.5%, OR=0.394, CI=0.194-0.798, p=0.013]. Also, among the three activating genes that may use HLA class I molecules as their ligands, a significant decrease was shown in frequency of KIR3DS1 with HLA-B Bw4[Ile80] ligand in TB patients compared to control group [4% vs. 23%, OR=0.14, CI=0.033-0.596, p=0.004]. These findings imply a genetic imbalance between activating and inhibitory KIR genes and KIR-HLA combinations in Lur TB patients. Low level of activating KIR3DS1 and its combination with HLA-B Bw4[Ile80] ligand might have an influence on the susceptibility to TB in Lur population of Iran