ABSTRACT
Objective To investigate the blocking effects of Pyrin domain protein on mouse allergic airway inflammation induced by ovalbumin and its mechanism.Methods Forty male BALB/c mice were randomly divided into 4 groups with 10 mice in each group.Control group:mice were treated with saline;OVA group:mice were sensitized and challenged with OVA; Pyrin domain protein 3 d group:mice were sensitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged; Pyrin domain protein 7 d group:mice were sensitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged.All mice were killed 24 h after final OVA challenge.The left lung was isolated for pathological examination.Lung sections were stained with hematoxylin and eosin(HE),Masson's trichrome.The thickness of bronchial airway,bronchial smooth muscle thickness,and the collagen deposition area were measured by image analysig system.The concentrations of IL-4,IL-5,TNF-α and IFN-γ in BALF were measured by ELISA,the RT-PCR and Western blot was performed to detect the mRNA expression of connective tissue growth factor (CTGF),TGF-β 1 mRNA and NF -kB from the right lung tissues.Results In OVA group,the number of inflammatory cells and the concentrations of IL-4,IL-5,TNF-a in BALF and Bronchial airway thickness,bronchial smooth muscle thickness,the collagen deposition area,CTGF,TGF-β1 mRNA and NF-kB in lung tissue were significantly higher than those in control group(P＜0.05).IFN-γ were lower than those in control group( P＜0.05 ).While in Pyrin domain protein 3 d group and Pyrin domain protein 7 d group,the number of inflammatory cells,the concentrations of IL-4,IL-5,TNF-α in BALF and bronchial airway thickness,bronchial smooth muscle thickness,the collagen deposition area and NF-kB in lung tissue were significantly lower than those in OVA group,IFN-γwere higher than those in OVA group( P ＜0.05).In Pyrin domain protein 7 d group CTGF and TGF-β1 mRNA in lung tissue were significantly lower than those in OVA group.Conclusion Pyrin domain protein might inhibits airway inflammation and development of airway remodeling in asthmatic mouse,the possible mechanism might in part by associated with reducing the expression of NF-kB and further inhibiting CTGF,TGF-β1.
ABSTRACT
Farmer's lung is a form of hypersensitivity pneumonitis, which is manifested mainly as an occupational disease among dairy farmers exposed to organic dusts from moldy hay and compost in silos and lofts. In Europe and America, it has been known for many years. In Japan, it frequently occurs in Hokkaido, Iwate and Hokuriku districts during winter months from November to March. The culprit antigens are spores of actinomycetes thermophilus such as Micropolyspora faeni and Thermoactinomyces vulgaris. In recent years, various preventive measures have been taken. For instance, in order to prevent hay from heating by fermentation and the thermophile from thriving, farmers desiccate hay as much as possible and keep it in air-tight plastic containers. The result is a notable decrease in the prevalence rate of this ailment. Nonetheless, with graying the rural population, pulmonary fibrosis caused by chronic exposure to low levels of organic dust is posing a grave health problem in the dairy farming communities. As a prophylactic measure against chronic fibrotic lung disease, there is a need to implement a comprehensive heath control program consisting of continual sanitary checkups at workplace and counseling at an early stage in addition to periodic health examinations. Another health threat which has more recently loomed up in the dairy farming communities is environmental pollution.