Characterization, In Vitro Antioxidant And Hepatoprotective Activity Of Fungal Endophytic Extracts Of Andrographis Paniculata Leaves In Ccl4 Induced Hepatotoxicity

Objective: To isolate and characterize the endophytic fungi from the leaves of Andrographis paniculata for free radical scavenging antioxidant and hepatoprotective activity against CCl4 induced hepatotoxicity Methods: Two fungal endophytes, APLF-3 (Andrographis paniculata leaf fungi-3) and APLF-4 (Andrographis paniculata leaf fungi-4) were isolated from leaves of Andrographis paniculata to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA sequencing by PCR technique was carried out for identification of APLF-3 and APLF-4. All the APLF-3 and APLF-4 extracts were assayed for in vitro free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power. Then, A4EA and A4nB were screened for hepatoprotective activity against CCl4 induced hepatotoxicity at 50 mg/kg and100 mg/kg doses. Results: The endophytic fungi, APLF-3 and APLF-4, were identified as Phyllosticta sp. ZLY-2010 isolate M13 and Aspergillus tubingensis strain Cs/7/2 respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant in vitro free radical scavenging activity as compared to other extracts. A4EA and A4nB (50 mg/kg and100 mg/kg) reversed the increased serum biochemical parameters as compared to CCl4 treated group (p<0.001). A4EA and A4nB (100 mg/kg p. o) also restored the LPO, SOD and CAT levels. Conclusion: These findings suggested that the extracts (A4EA and A4nB) obtained from endophytic fungi APLF-4 contributed towards hepatoprotective activity.

Phytochemical screening and evaluation of analgesic and antiinflammatory activities of Phaseolus vulgaris linn., seeds in rodents.

With an objective of developing some novel analgesic and antiinflammatory natural agents with fast acting and low toxicity profile here in, the different extracts of Phaseolus vulgaris (Linn) seeds were evaluated for analgesic and antiinflammatory activities using glacial acetic acid induced writhing and carrageenan induced rat paw oedema method respectively. For screening of the extracts for analgesic and antiinflammatory activities aspirin and diclofenac were used as standard drugs respectively. Petroleum ether extract exhibited significant analgesic and antiinflammatory activities. The petroleum ether extract can be considered as a potential candidate for analgesic and antiinflammatory activities. The presence of steroids and flavonoids in petroleum ether extract of Phaseolus vulgaris Linn., seeds could be attributed for the analgesic and antiinflammatory activities.

Phytochemical screening and evaluation of analgesic, anti-antiinflammatory activities of Peganum harmala Linn., seeds in rodents.

The objective of this research work is to carryout the phytochemical screening and evaluate the analgesic, antiinflammatory activities of Peganum harmala Linn., seeds. In this study different extracts of Peganum harmala (Linn) seeds were evaluated for analgesic and anti-inflammatory activities using glacial acetic acid induced writhing and carrageenan induced rat paw edema models respectively. For analgesic and anti-inflammatory activities aspirin and diclofenac were used as standard drugs respectively. The ethyl acetate extract showed significant analgesic and anti-inflammatory activities, thus it can be considered as a potential candidate for analgesic and antiinflammatory activities. The presence of alkaloids, steroids, flavonoids in ethyl acetate extract of Peganum harmala (Linn) seeds could be attributed for the claimed analgesic and anti-inflammatory activities.