ABSTRACT
The main aim of the present study was to evaluate analgesic and CNS depressant activity of methanolic extracts of stem and roots of Cissus pallida and aerial parts of Cissus vitegenia in experimental animals. The analgesic activity was evaluated by Eddy’s hot plate method and CNS depressant activity was evaluated by using digital actophotometer. The study was carried out by using two different doses (200 and 400mg/kg body weight) of both the extracts. The preliminary pharmacological screening showed that both the extracts showed moderate analgesic activity and significant CNS depressant activity.
ABSTRACT
Mini matrices containing Losartan potassium as a model drug were prepared by extrusion method using Hydroxy propyl methyl cellulose as matrix materials with or without xanthan gum and carbopol, and combination of xanthan gum and carbopol and propylene glycol as plasticizer. The prepared mini matrices were further evaluated for surface texture, uniformity of diameter, thickness, weight, moisture content, drug content uniformity, drug-excipients interaction, relative swelling, mucoadhesive test, in vitro drug release pattern. All the HPMC mini matrices showed good swelling which is proportional to concentration of polymer. The In vitro release of drug was in the range of 64.83% to 93.66%. Formulation H1 prepared with a drug-polymer ratio of 1:0.5 (LSP:HPMC) and 5% propylene glycol by weight of polymer as plasticizer showed promising results as a controlled release dosage form and released approximately 93.66% of the drug in 12 h. This study proves that extrusion method can be used for designing controlled release drug delivery systems providing nearly zero-order drug release over a period of 12 h.
ABSTRACT
Current study is to develop the colon targeted matrix tablet using the natural polysaccharide sterculia gum as carrier and model drug ciprofloxacin HCl. The matrix tablets were prepared by wet granulation technology using the various proportions of sterculia gum with carbopol 934 P, sterculia gum and ethyl cellulose polymer blends. Gra-nules of all formulations were evaluated for rheological, post compressional properties and in vitro dissolution study in different pH buffers of pH 1.2 , pH 7.4 , pH 6.8 (saline phosphate buffer) without and with 4% rat cecal content in order to mimic GIT condition . Formulation SGC2 to SGC4 and SGE7 to SGE9 has released 13.6% to 38.9% in the initial 5h and released more amount of drug in stomach and small intestine than colon. Formulation SGC5 containing 45% of sterculia gum and 25% carbopol 934 p and Formulation SGE10 containing 45% of sterculia gum and 25% ethyl cellulose has released minimum 10.91 % to 13.04 % in the initial 5h and sustained the drug release up to 24 h and at the end of study released 75% to 79.99%. Formulations with 4% rat cecal content at the end of 24 h study drug released is 90.44% to 95.33% indicating higher amount of drug release is due to enzymatic break down of sterculia gum in the matrix tablet. Hence the above results conclude that the formulation SGC5 and SGE10 are potential in targeting the drug to colon to treat irritable bowel disease.
ABSTRACT
Analytical method development and validation was useful for estimation of drugs in bulk and biological fluids. They help to improve the reliability, consistency and accuracy of analytical data. Present investigation involves development and validation of UV spectroscopic method for abacavir sulphate as per ICH guidelines. The present work describes sensitive and robust method development of abacavir sulphate by UV spectroscopic method for estimation of abacavir sulphate in bulk and pharmaceutical dosage forms using UV-1700 pharma spec (Shimadzu). The method was validated for accuracy, precision, linearity, ruggedness and robustness to check its consistency. The wavelength scan of UV spectroscopic method showed absorption maxima 249 nm obeying beers law with linearity range of 0-40μg/ml and correlation coefficient of 0.99939. Molar absorptivity and sandell’s sensitivity are found to be 1.2404472 ×10-4 and 0.0208 respectively. The accuracy was found to be 99.94%-100.2% with recovery 99.76%, 100.06%, 100.07% for 50%, 100%, 150% solutions. The obtained results indicated that the developed analytical method was sensitive, accurate, with low standard deviation values for all validation parameters and could be used in day to day regular analysis of abacavir sulphate in bulk and pharmaceutical formulations.
ABSTRACT
Current study is to develop the colon targeted matrix tablet using the natural polysaccharide sterculia gum as carrier and model drug ciprofloxacin HCl. The matrix tablets were prepared by wet granulation technology using the various proportions of sterculia gum with carbopol 934 P, sterculia gum and ethyl cellulose polymer blends. Gra-nules of all formulations were evaluated for rheological, post compressional properties and in vitro dissolution study in different pH buffers of pH 1.2 , pH 7.4 , pH 6.8 (saline phosphate buffer) without and with 4% rat cecal content in order to mimic GIT condition . Formulation SGC2 to SGC4 and SGE7 to SGE9 has released 13.6% to 38.9% in the initial 5h and released more amount of drug in stomach and small intestine than colon. Formulation SGC5 containing 45% of sterculia gum and 25% carbopol 934 p and Formulation SGE10 containing 45% of sterculia gum and 25% ethyl cellulose has released minimum 10.91 % to 13.04 % in the initial 5h and sustained the drug release up to 24 h and at the end of study released 75% to 79.99%. Formulations with 4% rat cecal content at the end of 24 h study drug released is 90.44% to 95.33% indicating higher amount of drug release is due to enzymatic break down of sterculia gum in the matrix tablet. Hence the above results conclude that the formulation SGC5 and SGE10 are potential in targeting the drug to colon to treat irritable bowel disease.