ABSTRACT
Background: The aim of the present study was to evaluate the antidiabetic potential of Manomani chooranam (MMC), an indigenous polyherbal siddha formulation in Streptozotocin (STZ) induced diabetic female Wistar albino rats.Methods: Aqueous extract of MMC was prepared. Wistar albino rats were divided into six groups (n=6). Group 1 was kept as normal control, Group 2, 3, 4, 5 and 6 were induced diabetes. After induction, the group 2 was kept as diabetes control; Group 3 received the standard drug metformin (100 mg/kg), whereas Groups 4, 5 and 6 were treated with the aqueous extract of MMC at 500 mg/kg, 1000 mg/kg and 1250 mg/kg doses, respectively for the 21 days. Blood sugar was estimated at the end of each week. At the end of the study, rats were sacrificed and the pancreas was analyzed for histopathological changes. Data expressed as mean±standard error of the mean. Statistical analysis was done using one-way ANOVA followed by post hoc Tukey's test. p<0.05 considered statistically significant.Results: The groups which received aqueous extract of MMC at 500 mg/kg, 1000 mg/kg and 1250 mg/kg showed a significant decrease in the mean blood sugar level when compared to normal level. The groups which received MMC shows significant reduction in blood sugar level in comparison with standard drug metformin 100 mg/kg.Conclusions: The aqueous extract of MMC was able to decrease the elevated blood sugar levels in dose dependent manner.
ABSTRACT
Background: Peptic ulcer disease is the most prevalent gastrointestinal diseases caused by an imbalance between gastric stimulant or aggressive factors and the mucosal defensive factors. The defence of flavonoids against the tissue oxidative stress is being proved in various animal models for wide pharmacological effects. The aim of the present study is to evaluate the anti-oxidant effect of quercetin in histamine induced gastric ulcers.Methods: Male guinea pigs were divided into 4 groups (n=6). Group I includes normal control. Group 2, 3 and 4 were induced gastric ulcers with histamine as intraperitoneal (IP) injection. Group 2 serves as the gastric ulcer control. Group 3 and Group 4 are pre-treated with quercetin 200 mg/kg per orally (PO) and ranitidine 100 mg/kg PO respectively 45mins before histamine injection. After 4 hours of histamine injection, the animals were sacrificed to collect blood samples and stomach tissue for estimation of plasma and tissue antioxidant levels.Results: On estimation of antioxidant levels both in plasma and stomach tissues the SOD and CAT levels increased in the Group 3 and 4 significantly and also a significant reduction in MDA levels were noted in the Group 3 and 4 compared to the gastric ulcer control group.Conclusions: Hence, with flavonoids quercetin utilization in histamine induced gastric ulcers, the antioxidants showed comparative levels with ranitidine treatment groups. So a permanent cure for the chronic gastric ulcers could be proved in further studies as this is the milestone, tough to achieve in general clinical practice.