ABSTRACT
Objective:To investigate the collateral circulation compensation model in patients with favorable prognosis of basilar artery occlusion/severe stenosis treated with drugs or endovascular therapy.Methods:Clinical data of patients with basilar artery occlusion/severe stenosis and good clinical outcome were retrospectively collected in the Department of Neurology, Sixth Medical Center of PLA General Hospital from January 2019 to January 2020. They were divided into intensive drug therapy group and combined endovascular therapy group. The number and ways of collateral compensation pathway described by digital substraction angiography (DSA) were analyzed, and the characteristics of the collateral compensation model were summarized. SPSS22.0 software was used for statistical analysis, and the constituent ratio (%) was used for statistical description of the enumeration data.Results:A total of 32 eligible patients were included, including 27 males and 5 females, with an average age 45-76 (59±10) years. The compensation model included posterior communicating artery-posterior cerebral artery (13 cases, 40.6%), posterior communicating artery-posterior cerebral artery-basilar artery (10 cases, 31.2%), cerebellar artery-anastomotic branches of superior cerebellar artery (8 cases, 25.0%), anterior choroid artery-anastomotic branches of posterior choroid artery (2 cases, 6.2%), collateral circulation not established (11 cases, 34.4%).In drug treatment group, collateral compensation was found in the majority (14/15), with mainly posterior communicating artery (10/14).Most patients in combined treatment group did not develop collateral compensation (10/17), anastomotic branches of PICA-SCA were the main routes (6/7).Conclusion:In patients with basilar artery occlusion/severe stenosis, favorable clinical outcome can be achieved in both groups of patients treated with intensive drug therapy or endovascular therapy.
ABSTRACT
The growth of fibroblasts on the acellular dermal matrix (ADM) was studied. The fibroblasts isolated from the skin of an adult New Zealand Rabbit were cultured in vitro and identified subsequently. After the cells were inoculated on the ADM as seeds, the adhesion rate and the growth ability were examined, and cellular morphology was assayed with DAPI fluorescent staining and Scanning electron microscope (SEM). The possibilities of applying ADM as cells carrier or deliverer in the field of transplantation were evaluated. The result revealed that pure fibroblasts were isolated through the specific method. Skin fibroblasts could adhere to ADM easily, and the adhesion rate was 96.78%, displaying no significant difference (P > 0.05) when compared with that rate of the control holes. The cells on the scaffolds and those on the control holes showed similar growth tendencies, but the activity of the former was lower (P < 0.01). The integral nucleus with blue fluorescence could be observed on the ADM under fluorescence microscope. The number of fibroblasts scaled up with the cultured time, The results of SEM showed that the state of cell was good and the fibroblasts were fused into a layer after being cultured for 5-10d. So rabbit fibroblasts can attach, survive, grow and proliferate on the ADM in a healthy way. It is entirely possible to use ADM as an appropriate scaffold material for the culture of fibroblasts and as a material for transplantations.
Subject(s)
Animals , Rabbits , Biocompatible Materials , Cell Adhesion , Cell Proliferation , Cells, Cultured , Dermis , Cell Biology , Fibroblasts , Cell Biology , Skin , Cell Biology , Skin, Artificial , Tissue Engineering , Methods , Tissue ScaffoldsABSTRACT
Konjac glucomannan-collagen-chitosan blend films were prepared successfully by the solvent-casting method and were characterized by FT-IR,X-ray diffraction, SEM and optical transmittance. Moreover, tensile strength, breaking extension, water absorption, water vapor permeation coefficients, adsorbability and penetrating rates were measured. The results indicated that some strong interaction and good compatibility existed among Konjac glucomannan /collagen and chitosan in the blend films. Some properties of the KCCS films were improved markedly in comparison with binary blend films or Konjac glucomannan, collagen and chitosan film. The results of culturing vessel endothelial cells on CKCS-5 film showed that the blend films have good cell compatibility which indicates the potential for a scalfold material in tissue engineering.
Subject(s)
Humans , Biocompatible Materials , Chemistry , Cells, Cultured , Chitosan , Chemistry , Collagen , Chemistry , Endothelial Cells , Cell Biology , Mannans , Chemistry , Materials Testing , Membranes, Artificial , Spectroscopy, Fourier Transform Infrared , Tensile Strength , Tissue Engineering , Methods , X-Ray DiffractionABSTRACT
This study aims at restoring the skin from traumatism by use of the collagen(from piglet skin) and konjac glucomannan-chondroitin sulfate blend film. The 2 cm x 4 cm skin traumatism model was established on both sides of the waist spinal column in 14 New Zealand rabbits each weighing 1.5-2.0 kg. One side was covered with blend film, the other side was used as a control. Then the changes of the skin traumatism were observed at different time-points after the operation, the wound tissue samples were taken for histological examination. The blend film could prevent skin traumatism from bleeding and infection. The skin traumatism treated by blend film showed signs of rectangle scab and the control showed signs of linear scab after healing. No obvious immune rejection was seen. The collagen-konjac glucomannan-chondroitin sulfate blend film can accelerate the restoration of skin from traumatism.