The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress / Atividade antioxidante do extrato das folhas de capim-limão contra o estresse oxidativo em fibroblastos

Objective : The purpose of this research is to assess the antioxidant activity of lemongrass leaves extract in terms of lowering ROS generation and its effect on the viability and proliferation of fibroblasts under oxidative stress. Material and Methods: The antioxidant activity was measured using the DPPH method and the ROS assay was carried out by fluorescent H2DCFDA staining. Viability and proliferation assays were performed using the Cell Counting Kit-8 (CCK-8) and was read at 450 nm using microplate reader. The groups were divided into 8, namely fibroblasts without treatment (comparison group), fibroblast induced by H2O2 (negative control), fibroblast with H2O2 then treated with ascorbic acid (positive control), and fibroblast with H2O2 then treated with lemongrass leaves extract at various concentrations (10, 20, 30, 40, and 50 ppm). Results: The results showed that the antioxidant activity of lemongrass leaves extract had an IC value of 64.17 ppm. ROS production were reduced by the LgLE of all concentrations if compared with negative control (p=0.819). LgLE can maintained the fibroblast viability with 10 ppm of LgLE was the most optimum concentration (p<0.05). LgLE can induced the proliferation of fibroblast, with the most effective was at 24 h of observation (p<0.05). Conclusion: Lemongrass leaves extract has a strong antioxidant activity that can reduce oxidative stress and increase the viability and proliferation of fibroblasts with the optimum concentration is at 10 ppm. (AU)

Objetivo: O intuito deste estudo foi determinar a ação antioxidante do extrato das folhas de capim-limão no que se refere a diminuição da produção de espécies reativas do oxigênio (EROS) e o seu efeito na viabilidade e proliferação de fibroblastos submetidos à estresse oxidativo. Material e Métodos: A atividade antioxidante foi medida utilizando o método de DPPH e o ensaio de EROS foi realizado pela coloração fluorescente de H2DCFDA. Os ensaios de proliferação e viabilidade foram realizados utilizando-se o kit de contagem de células CCK-8 em microplacas de leitura à 450nm. Os grupos foram divididos em 8: Fibroblastos sem tratamento (grupo controle), Fibroblastos tratados com H2O2 (controle negativo), Fibroblastos tratados com H2O2 e extrato da folha de capim-limão em concentrações variadas (10, 20, 30, 40 e 50 ppm). Resultados: Os resultados mostraram que a atividade antioxidante do extrato de capim-limão teve uma IC50 (com o numeroal subscrito) com valor de 64.17ppm. A produção de ROS foi reduzida pelo tratamento com o extrato em todas as concentrações testadas quando comparado ao grupo controle negativo (p=0.819). O extrato manteve a viabilidade dos fibroblastos, sendo 10ppm a concentração menos tóxica (p<0.05). LgLE pôde induzir a proliferação de fibroblastos, sendo que a melhor eficiencia foi após 24h de observação (p<0.05). Conclusão: O extrato das folhas de capim-limão apresentam forte atividade antioxidante reduzindo o estresse oxidativo e aumentando a viabilidade e proliferação de fibroblastos, sendo a concentração ótima de 10ppm. (AU)

Fenugreek (Trigonella foenum-graceum) increases postmenopausal fibroblast-associated COL1A1 and COL3A1 production dominantly through its binding to estrogen receptor beta

In postmenopausal women, oral or topical administration of estradiol increases skin thickness and collagen synthesis,such as collagen type 1 alpha 1 (COL1A1) and collagen type 3 alpha 1 (COL3A1). Due to undesirable side effectsof estradiol, such as risks of breast and endometrium pathology, topical phytoestrogens are alternative treatments foraging-related skin changes. Phytoestrogen is a nonsteroidal substance derived from plants, like fenugreek (Trigonellafoenum-graceum L.), which has an estrogen like composition that appears to mimic estradiol. The mechanism ofaction remains unknown, especially in fibroblast-associated COL1A1 and COL3A1 production. In vitro experimentswere conducted using postmenopausal women's fibroblasts with estrogen receptor (ER) antagonists. Cell isolationused explant and enzymatic techniques with ELISA kit (MyBioSource, California) for COL1A1 and COL3A1. Pairedstudent t-tests compared results between control (no treatment), fenugreek extract 2 µg/ml alone, fenugreek extract 2µg/ml with receptor antagonists for ERα, ERβ, and both receptors. Greater suppresion of COL1A1 and COL3A1 wereshown by both antagonists ERα / ERβ group and antagonist ERβ group compared to antagonist ERα group. Theseresults indicate that the fenugreek increases secretion of COL1A1 and COL3A1 through ERα, ERβ, and is mainlymediated by ERβ in post menopausal women’s fibroblasts.

Acalypha indica root extract increases post-hypoxic rat hippocampal tissue culture cell viability via phospholipase A2 inhibition.

Background: Phospholipase A2 (PLA2) is involved in inflammation and cell death following stroke, and inhibition of its activity may promote neuroregeneration. This study aimed to observe the influence of Acalypha indica Linn root extract towards relative cell viability and PLA2 enzyme level in post-hypoxic hippocampal tissue culture. Methods: Experimental in vitro study using 24 primary neuronal cell cultures obtained from Sprague Dawley rat exposed to hypoxia with 5% O2 / 5% CO2 / N2 balanced gas for 24 hours. Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to three treatment groups. No treatment was given to the control group. Each group consists of six samples. After 72 hours of incubation, relative cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and phospholipase A2 enzyme level was determined using ELISA. Results: PLA2 enzyme level of rat hippocampal tissue culture treated with Acalypha indica Linn root extract at 10, 15, and 20 mg/mL were significantly lower than that of control (5.55 ng/mL, 6.85 ng/mL, and 7.42 ng/mL vs 7.96 ng/mL, p < 0.05). Conclusion: Acalypha indica Linn root extract increases the relative cell viability and decreases the PLA2 enzyme level of post-hypoxic mouse hippocampal tissue with the optimal dose of the extract at 10 mg/mL.