Immunophenotypes of umbilical cord blood cells and GM-CSF mobilized peripheral blood cells / 대한임상병리학회지

BACKGROUDS: Different clinical outcomes of umbilical cord blood (CB) transplantation and GM-CSF mobilized peripheral blood stem cell (LB) transplantation stimulated our interests in immune characteristics of various stem cell sources so that we investigated immunophenotypes of mononuclear cells of twenty-six CB and ten LB cases and twenty normal adult peripheral blood (AB). METHODS: Two-color direct immunofluorescence analysis was performed using FACScan (Becton-Dickinson, USA) and monoclonal antibodies were CD34FITC/CD33PE, CD34FITC/CD45ROPE, CD34PE/CD45RAFITC, CD34PE/CD38FITC, CD4PE/CD45RAFITC, CD4FITC/CD45ROPE, CD8PE/CD45RAFITC, CD8PE/CD11bFITC, CD8PE/CD57FITC, CD8FITC/CD38PE (Serotec), and Simulset IMK lymphocyte kit (Becton-Dickinson). RESULTS: CD34+ cell frequencies of CB and LB were four times higher than that of AB. CD34+CD38- cell frequency was higher in CB, 41.5+/-28.1% compared to 20.1+/-15.8% in LB (P=0.0391) and frequencies of CD34+CD33+, CD34+CD45RA-, and CD34+CD45RO+ showed no difference. LB showed higher frequency of CD3+ and CD8+ cells and lower frequency of CD4+ and CD19+ cells compared to that of CB and AB. CD4+CD45RA+ na ve cell frequency was higher in CB (29.3%) compared to 2.3% of LB. CD4+CD45RO+ memory T lymphocyte frequency (8.5%) and CD8+CD57+ cell frequency (1.0%) were lowest in CB. CD8+CD45RA+ and CD8+CD11+ cell frequencies showed no difference. No statistically significant differences in CD34+ cell and its subsets and lymphocytes and their subsets were observed between normal vaginal delivered umbilical cord blood (VD) and Cesarean sectioned umbilical cord blood (CS), except CD8+CD11+ cell frequency was higher in VD (11+/-7.6%) compared to that (4.0+/-2.3%) in CS (P=0.0081). CONCLUSIONS: CB showed higher frequency of immature hematopoietic stem cell precursors and na ve lymphocytes compared to that of LB and AB and LB showed higher frequency of T lymphocytes and lower frequency of B lymphocytes compared to those of CB and AB, which might be the possible causes of the different clinical outcome. Study on the standard immunophenotypic values in various hematopoietic stem cell products using sufficient number of subjects is necessary for the guideline of clinical utilization.

Neglected Monteggia Fracture in Children: Report of Three Cases / 대한정형외과학회잡지

A neglected Monteggia fracture can pose a significant clinical problem because of decreased range of motion of the elbow, increased cubitus valgus, progressive damage and elbow instability. Factors leading to misdiagnosis include swelling of soft tissue, overlying of bones on radiographs and lack of inclusion of the elbow on the radiograph at the ulnsr fracture. Therefore, a radiograph that includes the elbow at the ulnar fracture should be taken. A line drawn through the shaft and head of the radius into the elbow should align with the capitulum in any projection, thereby assisting in the correct diagnosis. Three cases of neglected Monteggia fracture were treated by open reduction of the radial head with repair or reconstruction of the annular ligament and osteotomy of the ulna, and satisfactory results were obtained.

Clinical Study of Intra-articular Fracture of Distal Radius / 대한정형외과학회잡지

The treatment of intra-articular fractures of distal radius is difficult and has various problems. Maximum recovery of wrist function is dependent on accurate and stable reduction of the radial articular surface. We analysed one hundred and five cases of intra-articular fracture of distal radius followed for more than 6 months at Yonsei University from January 1980 to September 1988 and studied the result according to the type of treatment. The results of this study were as follows; 1. The incidence was high in female who aged over 6th decade, and in male who is active aged. 2. The main cause of this injury was fall down(34.3%). 3. In a group of closed reduction and cast immobilization, the functional result was satisfactory in 45.4%. 4. In a group of closed reduction and percutaneous pinning, the functional result was satisfactory in 50%. 5. In a group of open reduction and internal fixation, the functional result was satisfactory in 83. 3%. 6. In a group of external fixation, the functional result was satisfactory in 33.3%. 7. Overall result was mainly correlated with initial severity of the injury and anatomical reduction of the intra-articular surface.