ABSTRACT
Adnexal torsion is a disease occurring mostly in young fertile women that causes severe pain with necrosis of the adnexa requiring an emergency surgery. Because the symptoms and physical findings are similar to emergency diseases of adjacent organs such as appendicitis, diagnosis of adnexal torsion could be confused. Delayed diagnosis leads to delayed operation and for that reason adnexectomy is done more often than conservative management. Since prompt diagnosis is the sole way for preservation of the ovary and the salpinx, early diagnosis of adnexal torsion is essential. We experienced a case of a 16 year old female with torsion of the right adnexa who had the left adnexa previously removed due to torsion of the left adnexa. The case is presented with review of the literature.
Subject(s)
Adolescent , Female , Humans , Appendicitis , Delayed Diagnosis , Diagnosis , Early Diagnosis , Emergencies , Fallopian Tubes , Laparoscopy , Necrosis , OvaryABSTRACT
Peripartum cardiomyopathy is a dilated cardiomyopathy of unexplained cause that occurs during the last trimester of pregnancy or within 5 months after delivery. And, It is a rare disease that has a frequency of 1 in 1,300-15,000 deliveries. It has not yet been reported that peripartum cardiomyopathy has occurred in an preeclampsia woman superimposed on idiopathic thrombocytopenic purpura. Moreover, the association between idiopathic thrombocytopenic purpura and peripartum cardiomyopathy has not been studied. Recently, we experienced a case of a woman who has undergone emergency Cesarean section due to complicated severe preeclampsia superimposed on idiopathic thrombocytopenic purpura. On the postoperative day, this patient developed respiratory distress and pumonary edema. Peripartum cardiomyopathy was diagnosed by echocardiography and we present with a brief review of literatures.
Subject(s)
Female , Humans , Pregnancy , Cardiomyopathies , Cardiomyopathy, Dilated , Cesarean Section , Echocardiography , Edema , Emergencies , Peripartum Period , Pre-Eclampsia , Pregnancy Trimester, Third , Purpura, Thrombocytopenic, Idiopathic , Rare DiseasesABSTRACT
OBJECTIVE: To analyze the obstetric outcomes, the incidence of congenital malformations and chromosomal anomalies of neonates born after intracytoplasmic sperm injection (ICSI) according to the origin of sperm. METHODS: A total of 103 neonates born from ICSI between January 1995 and January 2004 was included. They were divided into three groups: ejaculated (n=73), epididymal (n=17) and testicular (n=13) groups. We compared obstetric outcomes such as pregnancy rates, preterm birth rates, abortion rates, multiple pregnancy rates, gestational age at birth, birth-weight, and the incidence of congenital malformations and chromosomal anomalies. RESULTS: There were no differences among the three groups in terms of pregnancy rates, preterm birth rates, abortion rates, multiple pregnancy rates, gestational age at birth and birth-weight. There were no differences in the incidence of low birth-weight babies and very low birth-weight babies. Major malformations were observed in two cases of ejaculatory group (2.7%) and one of testicular group (7.7%). Major malformations included two gastrointestinal malformations and one cleft lip. Minor malformations were observed in three cases of ejaculatory group (4.1%) and one of testicular group (7.7%). Chromosomal anomalies were observed in 6 cases of 105 pregnancies (5.7%), and all of them were from the ejaculatory group. Two cases had autosomal numerical anomalies and 4 cases autosomal structural anomalies. However, sex chromosomal anomalies were not detected in this study. CONCLUSION: The obstetric outcomes, the incidence of congenital malformations and chromosomal anomalies in ICSI babies were not different according to the origin of sperm. These findings should be further investigated in larger long-term studies.
Subject(s)
Child , Female , Humans , Infant, Newborn , Pregnancy , Abortion, Induced , Cleft Lip , Gestational Age , Incidence , Parturition , Pregnancy Rate , Pregnancy, Multiple , Premature Birth , Sperm Injections, Intracytoplasmic , SpermatozoaABSTRACT
OBJECTIVE: To explore the association of p53 codon 72 polymorphism with endometriosis. METHODS: Two hundred seventy-one women with surgically or histologically diagnosed edometriosis of stage I-IV, and 219 patients with no evidence of endometriosis by laparoscopy or laparotomy served as control. Allele frequencies and genotype distribution of p53 polymorphisms (arginine homozygosity, heterozygosity, and proline homozygosity) in affected women and controls were evaluated. RESULTS: The genotype distributions of p53 codon 72 polymorphisms did not differ significantly between endometriosis group and control group (p=0.086). However, the genotype distributions of p53 codon 72 polymorphisms differ significantly between stage I-II endometriosis group and control group (p=0.043). Proline homozygotes had higher risk for stage I-II endometriosis compared to arginine homozygotes (odds ratio=2.75, p=0.013). CONCLUSION: These results suggest that proline homozygote of p53 codon 72 polymorphism is associated with the risk of minimal or mild stage of endometriosis in the Korean population.
Subject(s)
Female , Humans , Arginine , Codon , Endometriosis , Gene Frequency , Genotype , Homozygote , Laparoscopy , Laparotomy , ProlineABSTRACT
OBJECTIVE: Cisplatin is a chemotherapeutic drug which is widely used for cancer therapy including ovarian cancer. The purpose of this study is to elucidate synergistic effect of Cispaltin and Theophylline on the apoptosis of OC-109. METHODS: Human ovarian cancer cell lines of epithelial origin (OC-109, OC-238) were used for experiments. Antibody of Bcl-2 gene was purchased by Santa Cruz Biotechnology, Inc. (Heidelberg, Germany) and p53 antibody and Mdm-2 antibody were generous gift from M. Oren at the Weizmann Institute (Rehovot, Israel). Apoptosis was measured by FACS analysis and TUNEL method. Western blot for protein expression and biochemical analysis for measuring protein was done by Bradford method. RESULTS: The inhibitory effect of Cisplatin on the cell growth began at the concentration of 0.3 microM and increased to 9 microM, while Gemzar began to inhibit cell growth at the concentration of 0.0013 microM and increased to 0.12 microM. Theophylline did not show significant inhibitory effect until the concentration of 80 microgram/mL, but the cell growth was inhibited significantly at the concentration of 320 microgram/mL. Synergistic effect of Cisplatin and Theophylline was observed at the concentration of 1 microM and 80 microgram/mL, respectively. Apoptosis was measured by the shifting to sub-G1 phase of cell cycle by FACS analysis. Synergistic effect of Cispatin, Gemzar and Theophylline was observed. TUNEL assay also showed clear synergistic effect of Cisplatin and Theophylline. Bcl-2 gene product which inhibits the apoptosis was down-regulated after Cisplatin, Cisplatin and Theophylline co-treatment. Mdm-2 gene product was up-regulated while p53 protein was not changed significantly. CONCLUSION: Synergistic effect of Cisplatin and Theophylline on the inhibition of cell growth by apoptosis was clearly observed. This effect suggest the possibility lowering the concentration of chemotherapeutic drugs, which alleviate the side effect of drugs. Bcl-2 gene expression was down-regulated after Cisplatin and Theophylline co-treatment while Mdm-2 gene expression was up-regulated. p53 gene expression was not significantly changed after stimulation of various combination. Apoptosis was measured by FACS analysis and TUNNEL assay, which showed clearly synergistic effect of Cisplatin and Theophylline.
Subject(s)
Humans , Apoptosis , Biotechnology , Blotting, Western , Cell Cycle , Cell Line , Cisplatin , Gene Expression , Genes, bcl-2 , Genes, p53 , In Situ Nick-End Labeling , Ovarian Neoplasms , TheophyllineABSTRACT
INTRODUCTION: Apoptosis is a naturally occurring cell suicide pathway. The absence of a survival factor, such as a particular hormone or growth factor, will induce a cell to initiate its own cell death. This process induced in osteoblast is thought to play a role in the pathophysiology of postmenopausal osteoporosis. Estrogen plays an important role and exerts direct effects on bone cells, but the role in the maintenance of cell survival and apoptosis is poorly understood. Based on the importance of survival factors and cytokines in bone remodeling, some studies suggest that estrogen acts on bone cell by modulation of cytokine production that increases bone resorption. However other studies have not investigated the effect of estrogen on the apoptosis of osteoblast cells. OBJECTIVE: To understand how estrogens regulate the apoptosis of osteoblast cells, the physiologically active etrogen metabolite 17 beta-estradiol was tested to determine its effects on the well characterized murine osteoblastic cell-line MC3T3-E1. METHODS: Experiments were designed to identify the effects of estrogen on TNF-alpha induced apoptosis and cytokine gene expression, and collagen synthesis. RESULTS: 1.Within 48 hours of exposure, recombinant murine at 10(-11)M - 10(-10)M TNF-alpha increased the rates of DNA synthesis and 10(-11)~M - 10(-9)M TNF-alpha reduces cell proliferation. 2.Estrogen treatment coordinately increased DNA contents, cell proliferation and collagen synthetic activity. 3.10(-10)M TNF-alpha increased the IL-1beta, IL-6 gene expression. 4.Estrogen attenuated the TNF-alpha- dependent increase in these gene expression. 5.Murine osteoblastic MC3T3-E1 cells underwent apoptosis following removal of serum, or addition of TNF-alpha, as indicated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and DNA fragmentation studies. 6.Estrogen inhibited the apoptosis of osteoblast cells induced by 10(-10)M TNF-alpha but not to the levels observed in those control. CONCLUSION: This in vitro evidence suggests that estrogen might exert at least part of antiapoptotic inflence on the bone cells.