In Vitro Effects of 1,25-Dihydroxyvitamin D3 on the Production of Interleukin-1alpha by Ultraviolet B Irradiation in Cultured Human Keratinocyte Cell Line HaCaT Cells / 대한피부과학회지

BACKGROUND: Keratinocyte-derived interleukin-1(IL-1)alpha is one of the key cytokines in initiation of cutaneous inflammation. Release of IL-1alpha from human keratinocytes may be induced by proinflammatory stimuli including ultraviolet B(UVB) irradiation, and subsequently, keratinocyte-derived IL-1alpha may exert numerous paracrine and autocrine effects. 1,25-dihydroxyvitamin D3(1,25(OH)2D3) is involved in the regulation of keratinocyte proliferation and differentiation and is also recognized to have immunoregulatory properties such as an antiinflammatory effect. OBJECTIVE: The purpose of this study was to investigate the in vitro effects of 1,25-(OH)2D3 on the production of IL-1alpha by UVB irradiation in cultured human keratinocyte cell line HaCaT cells. RESULTS: are summerized as follows; 1. The vialility of cultured HaCaT cells measured by MTS assay at 24 hours after UVB irradiation was significantly reduced at the doses of above 100 mJ/cm2 of UVB(p<0.05). 2. The secretion of IL-1alpha by HaCaT cells was significantly increased at the doses of above 30 mJ/cm2 of UVB(p<0.05). UVB irradiation could not influence on the secretion of IL-1beta by HaCaT cells. 3. At the concentrations of 10-8M and 10-6M of 1,25(OH)2D3, the production of IL-1alpha by HaCaT cells(48 hours after 100 mJ/cm2 UVB irradiation) was significantly inhibited in both culture supernatants and cell lysates(p<0.05). CONCLUSION: UVB irradiation increased the production of IL-1alpha by HaCaT cells and this stimulatory effect on the production of IL-1alpha induced by UVB irradiation was suppressed by 1,25-(OH)2D3. Calcipotriol(MC-903) had similar suppressive effect on the production of IL-1alpha induced by UVB irradiation in HaCaT cells to that of 1,25(OH)2D3.

Lipomembranous Fat Necrosis Associated with Vascular Insufficiency / 대한피부과학회지

Lipomembranous fat necrosis is a special form of fat necrosis characterized by the presence of cystic cavities lined by crenelated hyaline membranes. Lipomembranous fat necrosis has been observed in the subcutaneous tissue associated with various conditions, mainly with ischemia. The term, nodular-cystic fat necrosis was coined to describe a characteristic form of encapsulated subcutaneous fat necrosis resulting form encapsulated lipoma associated with trauma or ischemia. We herein report a case of lipomembranous fat necrosis associated with vascular insufficiency and possibly associated with nodular-cystic fat necrosis, and discuss the common pathogenesis of the two disorders.

Cutaneous Manifestations of Kidney Transplant Recipients / 대한피부과학회지

BACKGROUND: Kidney transplantation is now an accepted form of treatment for chronic renal failure. However, the necessary and prolonged use of immunosuppressive agents result in various complications. OBJECTIVE: The aim of the present study was to see what the mucocutaneous manifestations would be and how they develop in renal transplant recipients treated with immunosuppressants. METHODS: 187 patients who were examined in dermatology among kidney transplant recipients at Asan Medical Center between June 19S9 and August 1998 were included in this study. The clinical records were reviewed, physical examinations were conducted and the cultures or histological examinations were done when indicated. RESULTS: The most common findings in the skin of the renal transplant recipients were hypertrichosis and Cushingoid changes. The incidence of warts was 22%. There were three cases of Kaposis sarcoma, Bowens diseases, and porokeratosis. CONCLUSIONS: The incidence of skin cancers and precancerous lesions was lower than other countries, but cosmetic and functional complications of immunosuppression are frequent. Therefore, it is essential that all renal transplant recipients should be provided with initial and continuing periodic examinations by dermatologists.

Immunohistochemical study on granulomatous skin deseases / 대한피부과학회지

BACKGROUND: A definition of granuloma is a focal chronic inflammatory response to tissue injury evolved by a poorly soluble substwice characterized by the accumulation and proliferation of the mono-nuclear histiocytic cells. The accuracy with which rnononuclear cells may be identified in skir. is much improved by the use of both heteroantisera and monoclonal antibodies directed against selected cellular antigens, OBJECTIVE: Our purpose was to examine the staining patterns of anti-lysozyme, anti-a-1-antitrypsin, anti-S-100 protein antibodies, and MAC-387 monoclonal anibody in granulomatous skin diseases. METHOD: We performed imminoperoxidase staining(the labelled str prvidin-biotin peroxidase complex method on the formalin-fixed, paraffin-embedded tissue specimens of granulomatous skin diseases. RESULTS: S-100 protein positive dendritic cells were demonstrated in the granulomatous infiltrates as scattered pattern and MAC-387 positive cells were predominantly found in the center of granulomas, The staining pattern and percentage of positively stained cells of a--antitrypsin were similar to those of lysozyme. A1Pha-1-antitrypsin and lysozyme positive cells w re present in the center as well as lymphohistiocytic infiltrates of granulomas. CONCLUSION: These data sugget that histiocytes are composed of heter igeneous groups of cells such as the mononuclear-phagocyte system and dendritic cell system.