Single nucleotide polymorphism at rs7903146 of transcription factor 7-like 2 gene among subjects with type 2 diabetes mellitus in Myanmar / Journal of the ASEAN Federation of Endocrine Societies

Objectives@#To investigate the association between the single nucleotide polymorphism (SNP) rs7903146 in the transcription factor 7-like 2 (TCF7L2) gene and type 2 diabetes mellitus (T2DM) and to examine the impact of this variant on pancreatic beta-cell function in the Myanmar population.@*Methodology@#A case-control study was undertaken in 100 subjects with T2DM and 113 controls. The SNP rs7903146 was genotyped using the allele-specific polymerase chain reaction method. Plasma glucose and serum insulin levels were determined using the enzymatic colorimetric method and ELISA respectively. Beta-cell function was calculated by the HOMA-β formula.@*Results@#The frequencies of carrier genotypes (CT and TT) were higher in subjects with T2DM than in controls. The minor T alleles of rs7903146 were found to statistically increase type 2 diabetes risk than the C allele with an allelic odds ratio of 2.07 (95% CI 1.39-3.09, p=0.0004). The mean HOMA-β level of the group with non-carrier genotype (CC) was significantly higher than that of the groups with carrier genotypes (CT and TT) in subjects with T2DM and controls with a p-value of 0.0003 and less than 0.0001, respectively.@*Conclusion@#The rs7903146 variant of the TCF7L2 gene was found to be associated with T2DM and low β-cell function among Myanmar subjects.

Single Nucleotide Polymorphism at +276 g>T of the Adiponectin gene and Plasma Adiponectin Level in Myanmar Type 2 Diabetic Patients / Journal of the ASEAN Federation of Endocrine Societies

Objective@#The aim of the study was to investigate the association between single nucleotide polymorphisms (SNP) at rs 1501299 (SNP+276 G>T) of the adiponectin gene and plasma adiponectin levels in type 2 diabetes mellitus (T2DM) patients in Myanmar.@*Methodology@#One hundred T2DM patients and 104 non-diabetic subjects were included in this cross-sectional analytical study. Genotype frequencies were determined by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method. Plasma adiponectin level was measured by enzyme-linked immunosorbent assay (ELISA). @*Result@#Genotype frequencies (GG, GT, TT) of SNP+276 in diabetic patients were 39%, 48% and 13%, respectively. The GT and TT genotypes were more frequent in T2DM patients (OR 1.98, 95% CI, 1.10-3.55; p=0.02 and OR 4.07, 95% CI, 1.34-12.3; p=0.01), respectively. The T allele of SNP+276 was significantly associated with T2DM (OR 1.96, 95% CI, 1.27-3.01; p=0.002). Mean plasma adiponectin level was significantly lower than in T2DM patients (27.41±16.7 μg/mL) compared to non-diabetic subjects (37.19±26.77 μg/mL) (p=0.002)@*Conclusion@#SNP+276 at rs 1501299 of the adiponectin gene was associated with type 2 diabetes and low plasma adiponectin levels in this Myanmar population.

A study of lipid profile in Myanmar smokers and nonsmokers

This study was carried out to see the levels of serum lipids in smokers and nonsmoders. A total of 155 healthy subjects from the staff of the Institute of Medicine II, Yangon were studied. Among the 155 subjects, 96 were nonsmokers and 59 were active current smokers, smoking 1-9 cigarettes a day. Over night fasting samples of serum total cholesterol, serum HDL cholesterol, serum trigly-cerides and serum phospholipids were measured. It was found that smokers had significantly higher level of serum total cholesterol than nonsmoders.