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1.
Annals of Laboratory Medicine ; : 388-395, 2019.
Article in English | WPRIM | ID: wpr-739133

ABSTRACT

BACKGROUND: It is very important to accurately enumerate CD34-positive (CD34+) cells for successful hematopoietic stem cell transplantation (HSCT). We evaluated the ability of the newly developed image based-immunofluorescence cell counter ADAMII (NanoEntek, Seoul, Korea) to enumerate CD34+ cells, which was improved through simultaneous CD45 analysis. METHODS: We enumerated CD34+ cells with ADAMII using 19 peripheral blood (PB) and 91 leukapheresis samples from HSCT donors. Analytical performance, including precision and linearity, was analyzed, and sample stability during storage was evaluated. Viable CD34+ cell count (vCD34) and viable CD45+ cell count (vCD45) and the percentage of viable CD34+ cells among viable CD45+ cells (CD34/CD45) as measured by ADAMII were compared with the corresponding values from two flow cytometry assays, using regression analysis. RESULTS: ADAMII demonstrated acceptable precision, as CV values of vCD34 from six samples with different counts were all < 10% (range: 3.49–9.51%). CV values of the vCD45 and CD34/45 ranged from 4.03% to 9.67% and from 2.48% to 10.07%, respectively. The linearity of vCD34 showed an excellent R 2 value (0.99) when analyzed using the intended count and flow cytometry data. The ADAMII and two flow cytometry-based assays generated very similar data for the PB and leukapheresis samples. CONCLUSIONS: ADAMII demonstrated excellent performance for use as a routine clinical assay in terms of CD34+ cell enumeration from PB and leukapheresis samples. Moreover, it could be used as a point-of-care-test for determining mobilization time and predicting an adequate apheresis stem cell product.


Subject(s)
Humans , Blood Component Removal , Cell Count , Flow Cytometry , Fluorescence , Hematopoietic Stem Cell Transplantation , Leukapheresis , Seoul , Stem Cells , Tissue Donors
2.
Journal of Korean Academy of Nursing ; : 437-445, 2014.
Article in Korean | WPRIM | ID: wpr-150509

ABSTRACT

PURPOSE: This study was done to identify pain perception (P-PER) by nurses and pain expression (P-EXP) by patients in critical care units (ICUs) and degree of agreement between nurses' P-PER and patients' P-EXP. METHODS: Nurses' P-PER was measured with a self-administered questionnaire completed by 99 nurses working in ICUs during May, 2013. Patients' P-EXP was measured with the Critical Care Non-Verbal Pain Scale through observations of 31 ICU patients during nine nursing procedures (NPs) performed between May and July, 2013. RESULTS: Nurses' P-PER was from 4.49 points for nasogastric tube (NGT) insertion to 0.83 for blood pressure (BP) measurement based on a 9-point scale, Patients' P-EXP was 4.48 points for NGT to 0.18 for BP measurement based on a 10-point scale. Eight NPs except oral care showed higher scores for nurses' P-PER than for patients' P-EXP. Position change (p=.019), subcutaneous injection (p<.001), blood sugar test (p<.001), and BP measurement (p<.001) showed significant differences between nurses' P-PER and patients' P-EXP. CONCLUSION: Nasogastric tube (NGT) insertion was scored highest by both nurses and patients. Eight NPs except 'oral care' showed nurses' P-PER was higher or similar to patients' P-EXP, which indicates that nurses may overestimate procedural pain experienced by patients.


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Airway Extubation , Intensive Care Units , Intubation, Gastrointestinal , Nursing Assessment , Nursing Staff, Hospital/psychology , Pain Measurement , Pain Perception , Surveys and Questionnaires
3.
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 42-45, 2014.
Article in Korean | WPRIM | ID: wpr-647656

ABSTRACT

Extranodal NK/T cell lymphoma, nasal type, is a rare non-Hodgkin lymphoma originating in the nasal cavity or in the paranasal sinuses. Atrophic rhinitis is a debilitating chronic nasal mucosal disease of unknown etiology. However, there have not been any reports on the occurrence of nasal NK/T cell lymphoma in patients with atrophic rhinitis. We experienced a case of the extranodal NK/T cell lymphoma in the left nasal cavity, which had been misdiagnosed as atrophic rhinitis in a 39-year-old female. Thus, we report this rare case with a review of the literature.


Subject(s)
Adult , Female , Humans , Lymphoma , Lymphoma, Non-Hodgkin , Nasal Cavity , Paranasal Sinuses , Rhinitis, Atrophic
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