Comparison of Risk Factors for Type 2 Diabetes Mellitus in One-Person Households and Multi-Person Households

BACKGROUND: Recently, the number of one-person households has increased, and the prevalence of type 2 diabetes mellitus has risen in Korea. Major complications of diabetes, such as stroke and myocardial infarction are major causes of death. Therefore, we conducted this study to test the hypothesis that the risk factors and prevalence of type 2 diabetes mellitus differ between one-person and multi-person households.METHODS: This retrospective study analyzed data of 3,691 adults over 19 years old from the 7th (2016) Korea National Health and Nutrition Examination Survey. Data were analyzed by composite sampling for age, family history, waist circumference, body mass index, hypertension, high-density lipoprotein cholesterol, triglycerides, smoking, alcohol consumption, physical activity, and consumption of breakfast. Multivariate logistic regression analysis was performed by household-type in order to estimate the relative risk of factors associated with diabetes.RESULTS: Hypertension was a significant risk factor for diabetes in both groups. Among other diabetic risk factors, individuals in one-person households were more likely to skip breakfast and less likely to engage in physical activity than those in multi-person households.CONCLUSION: One-person households have a high risk of hypertension, skipping breakfast, and poor physical activity. It is important to consider the role of one-person households when studying the management and treatment of disease.

Alteration of Ryanodine-receptors in Cultured Rat Aortic Smooth Muscle Cells

Vascular smooth muscle cells can obtain a proliferative function in environments such as atherosclerosis in vivo or primary culture in vitro. Proliferation of vascular smooth muscle cells is accompanied by changes in ryanodine receptors (RyRs). In several studies, the cytosolic Ca2+ response to caffeine is decreased during smooth muscle cell culture. Although caffeine is commonly used to investigate RyR function because it is difficult to measure Ca2+ release from the sarcoplasmic reticulum (SR) directly, caffeine has additional off-target effects, including blocking inositol trisphosphate receptors and store-operated Ca2+ entry. Using freshly dissociated rat aortic smooth muscle cells (RASMCs) and cultured RASMCs, we sought to provide direct evidence for the operation of RyRs through the Ca2+- induced Ca2+-release pathway by directly measuring Ca2+ release from SR in permeabilized cells. An additional goal was to elucidate alterations of RyRs that occurred during culture. Perfusion of permeabilized, freshly dissociated RASMCs with Ca2+ stimulated Ca2+ release from the SR. Caffeine and ryanodine also induced Ca2+ release from the SR in dissociated RASMCs. In contrast, ryanodine, caffeine and Ca2+ failed to trigger Ca2+ release in cultured RASMCs. These results are consistent with results obtained by immunocytochemistry, which showed that RyRs were expressed in dissociated RASMCs, but not in cultured RASMCs. This study is the first to demonstrate Ca2+ release from the SR by cytosolic Ca2+ elevation in vascular smooth muscle cells, and also supports previous studies on the alterations of RyRs in vascular smooth muscle cells associated with culture.

Ca(2+)-induced Ca2+ Release from Internal Stores in INS-1 Rat Insulinoma Cells

The secretion of insulin from pancreatic beta-cells is triggered by the influx of Ca2+ through voltage-dependent Ca2+ channels. The resulting elevation of intracellular calcium ([Ca2+]i) triggers additional Ca2+ release from internal stores. Less well understood are the mechanisms involved in Ca2+ mobilization from internal stores after activation of Ca2+ influx. The mobilization process is known as calcium-induced calcium release (CICR). In this study, our goal was to investigate the existence of and the role of caffeine-sensitive ryanodine receptors (RyRs) in a rat pancreatic beta-cell line, INS-1 cells. To measure cytosolic and stored Ca2+, respectively, cultured INS-1 cells were loaded with fura-2/AM or furaptra/AM. [Ca2+]i was repetitively increased by caffeine stimulation in normal Ca2+ buffer. However, peak [Ca2+]i was only observed after the first caffeine stimulation in Ca2+ free buffer and this increase was markedly blocked by ruthenium red, a RyR blocker. KCl-induced elevations in [Ca2+]i were reduced by pretreatment with ruthenium red, as well as by depletion of internal Ca2+ stores using cyclopiazonic acid (CPA) or caffeine. Caffeine-induced Ca2+ mobilization ceased after the internal stores were depleted by carbamylcholine (CCh) or CPA. In permeabilized INS-1 cells, Ca2+ release from internal stores was activated by caffeine, Ca2+, or ryanodine. Furthermore, ruthenium red completely blocked the CICR response in permeabilized cells. RyRs were widely distributed throughout the intracellular compartment of INS-1 cells. These results suggest that caffeine-sensitive RyRs exist and modulate the CICR response from internal stores in INS-1 pancreatic beta-cells.

Shape and innervation of popliteus muscle / 대한해부학회지

The aim of this study was to delineate the shape of the popliteus muscle and determine the correct motor point site for treating spasticity. A total of 22 legs from 13 fresh Korean cadavers were evaluated. The x-axis was set as a transverse line across the lateral and medial epicondyle of the femur and the y-axis as a vertical line at the midpoint of the medial malleolus of the tibia and lateral malleolus of the fibula. The popliteus muscle is an obtuse triangle in shape. Superior, medial, and inferior angles were 27.2+/-4.3degrees, 114.8+/-19.8degrees, and 38.0+/-18.8degrees respectively. The lengths of the superior, medial, and lateral sides of the triangle were 7.6+/-1.0 cm, 6.2+/-1.0 cm, and 11.9+/-1.5 cm respectively. Nerve branches ran superficially on the periosteum of the tibia and entered the popliteus on its superficial surface. The diverging point of the nerve branch entered the popliteus from the tibial nerve located at the midline of the popliteal fossa and 17% of the leg length above the intercondylar line. Most nerve entry points (83.3%) were within a 2.0x3.0 cm rectangle with the center located at -1.0 cm (-7%) on the x-axis and -3.3 cm (-9%) on the y-axis.

Change of Stretch Reflex in Spinal Cord Injured Rabbit: Experimental Spasticity Model

OBJECTIVE: The purpose of this study is to develop an experimental spinal cord injury spasticity model using rabbits and to evaluate a quantitative spasticity measure. METHOD: After an general anesthesia, 19 rabbits out of total 24 rabbits were laminectomized posteriorly and the spinal cords contused with 15 g 20 cm weight drop around 11th and 12th thoracic cord. After two weeks, behavioral analysis and clinical measurements of hindlimb spasticity were assessed. After that, rabbits' triceps surae was dissected and the length-tension plot was obtained by stretching it 5 mm, 2 mm/sec. And then the stiffenss of stretch reflex was determined from the length-tension plot. The stiffness of stretch reflex of the five normal control group was measured. RESULTS: 1) The measure was possible among 15 out of 19 spinal cord injured rabbits. One out of 19 rabbits was died from urinary infection. Two rabbits was died from intraoperative bleeding at 2 weeks. Another one was excluded from the measurement due to joint contracture. 2) All of 15 rabbits showed clinical spasticity of hindlimb in 2 weeks. The spasticity was increased after the 4th day. 3) On the length-tension plot, the stiffness of stretch reflex of 15 rabbits significantly increased more than that of 5 normal group. 4) The total stiffness is correlated with the deep tendon reflex of knee and muscle tone of ankle. The stiffness of stretch reflex is weakly correlated with the deep tendon reflex of knee, digital hyperreflexia and ankle clonus. CONCLUSION: The experimental spinal cord injury model and the quantitative spasticity measure method would contribute to measure the effect of the new therapy of spasticity more accurately, and to establish a principle of the new therapy. In addition, the study is expected to contribute to establish pathophysiology of spasticity.

A Study on Hepatitis B Surface Antigen in Tear of Hepatitis Patients

We performed the radioimmunoassay to detect the hepatitis B surface antigen(HBsAg) in the tear. Among 70 patients who have positive reactions for HBsAg in blood, 53 patients(75.71%) were positive for HBsAg in tear. Among the various groups of hepatitis, the most positive reaction for HBsAg in tear was higher in serum HBeAg positive group(92.68%) than in serum HBeAg negative group(51.72%). So we emphasize the possibility of transmission of hepatitis B virus infection via tear.

A Study on Hepatitis B Surface Antigen in Tear of Hepatitis Patients

We performed the radioimmunoassay to detect the hepatitis B surface antigen (HBsAg) in the tear. Among 70 patients who have positive reactions for HBsAg in blood, 53 patients (75.71 %) were positive for HBsAg in tear. Among the various groups of hepatitis, the most positive reaction for HBsAg in tear was higher in serum HBeAg positive group (92.68%) than in serum HBeAg negative group (51.72%). So we emphasize the possibility of transmission of hepatitis B virus infection via tear.

Muscle Fiber Hypertrophy Following Monocular Medial Rectus Recession and Lateral Rectus Resection in Rats

Compensatory hypertrophy of muscle fibers has been known to occur when burden on a skeletal muscle is increased. However, there have not been reports concerning the response of extraocular muscles following a monocular resection and recession of horizontal muscles, which procedure is a common form of strabismus surgery. A large lateral rectus resection and medial rectus recession was performed in each right eye of 28 rats. After the horizontal muscle surgery, both medial and lateral rectus muscles were obtained from both eyes of each rat with an interval of 2 weeks from the initial operation until 8th week after operation. A mid-portion of an extirpated muscles were frozen and prepared for ATPase staining. With a differential preincubation in two buffered solutions, pH 4.6 and pH 10.3 and ATPase staining, muscle fibers were classified into 3 kinds-global singly innervated fiber(GSIF, alkali-stable), global multi-innervated fiber(GMIF, alkali-labile), and orbital surface layer fiber(OSLF). These muscle fibers were measured using micrometer to find out their diameters and compared with the diameter of extraocular muscle fibers of unoperated rats. All kinds of muscle fibers of a resected lateral rectus showed an increase in their diameteris(p<0.05, Wilcoxon rank sum test) but there was no hypertrophy in the recessed medial rectus the yolk muscle of the resected lateral rectus, medial rectus of the fellow eye also showed hypertrophy of GMIF and OSLF. The lateral rectus of the unoperated fellow eye did not reveal muscle fiber hypertrophy. The postoperative hypertrophy of muscle fibers were observed only at the 2nd and 4th postoperative week specimens. the hypertrophied muscles subsequently returned to the baseline fiber diameter.

Treatment of experimental vitreous hemorrhage with tissue plasminogen activator

Tissue plasminogen activator(tPA) is a fibrin-specific fibrinolytic agent that has recently been shown to be effective in accelerating the clearance of hyphema. Intravitreal injection of tPA can promote rapid lysis of experimental intravitreal fibrin clots. The purpose of this study was to investigate the efficacy of intravitreal tPA injection for the treatment of vitreous hemorrhage in normal phakic non-vitrectomized rabbit eyes. Vitreous hemorrhages were produced by intravitreal injections of 0.05 ml of autologous whole blood in 25 rabbit eyes with intact vitreous. The injection of 25 or 100 micrograms of tPA in 15 eyes resulted in the clearance of vitreous hemorrhage in 99 +/- 19 or 34 +/- 6.5 days, respectively. This was significantly faster than in the control eyes in which the clearance was not seen until 131 +/- 17 days later. No tractional retinal detachment was observed.

Binocular Luminance Summantion in Estoropic and Exotropic Kittens

Nomal kittens showed a gradual increase from 17.6% to 25.5% of BLS with aging from the fifth week to the ninth. Young kittens showed significantly less BLS than adult ones(p<0.1). There was no significant difference in BLS between exotropic and nomal kittens or alternating and non-alternating strabismic ones. However, there was a significant difference in BLS between esotropic and nomal ones(p<0.1). These results suggested that the BLS can be used as an index for the extent of total field from both eyes.

A Case of Endodermal Sinus Tumor of the Orbit

A 3-year-old girl with rapidly progressive proptosis had an unusual malignant germ cell tumor of the orbit identified as an endodermal sinus tumor. The endodermal sinus tumor is a malignant germ cell neoplasm that usually arises in gonads, but on rare ocassion ocurrs in extragonadal locations. Histologic examination revealed pseudo papillary pattern that on occasion created peculiar organoid structure similar to the glomeruli(Shiller-Dubal bodies). The patient was treated with exenteration of left orbit and three drug chemotherapy. The present case is of particular interest because of the rarity of extragonadal endodermal sinus tumor.

Clinical Evaluation of Sensory Heterotropia

Sensory Heterotropia is defined as a secondary deviation following loss or severe reduction in visual function in one eye. We have analyzed the records of 68 patients with sensory heterotropia in order to find the incidence, etiologic causes, the factors influencing the direction of the deviation, and the amount of deviation. The incidence of sensory heterotropia was 1.1% of total out patients, and 10.5% of strabismic patients. The major causative factors of sensory heterotropia was anisometropia(33.8%) followed in frequency by uncorrected aphakia(25.0%), cataract(16.2%), retina and vitreous diseases(16.2%), optic nerve atrophy(8.8%) and corneal opacity(2.9%). Esotropia and exotropia were encountered with almost equal frequency when the onset of visual impairment occured between birth and 5 years of age, but exotropia predominated in older children and adult. Those was statistically significant(p<0.01). The direction of a sensory heterotropia was determined by the refractive error of the sound eye, i.e., if the sound eye was emmetropic or myopic, thd blind eye was predominantly exotropic, and if it is hyperopic, it was predominantly esotropic. Also, duration of visual impairment was related to the amount of deviation in exotropic patients. If the duration was under 5 years, the amounts of deviation was 16.3 prism, and over 5 years, it was 25.5 prism, and difference was statistically significant.

Binocular luminance summation (BLS) in esotropic and exotropic kittens

Normal kittens showed a gradual increase from 17.6% to 25.5% of BLS with aging from the fifth week to the ninth. Young kittens showed significantly less BLS than do adult ones (p < 0.1), There was significant difference of BLS between exotropic and normal kittens or alternating and non-alternating strabismic ones. However, there was a significant diftrence between esotropic and normal ones (p < 0.1). These results suggested that the BLS can be used as a index for the extent of total field from both eyes.