ABSTRACT
The purpose of this study is to investigate the effect of artificial dermis(Terudermis(R)) on cartilage induction from perichondrium. A total of 24 rabbits were used and divided into control(n = 12) and experimental groups(n = 12). Each group was divided into 2 weeks(n = 6) and 4 weeks subgroups(n = 6). The dorsal skin of the rabbit ear was incised in reverse L-shape and the perichondrium was exposed. The silicone membrane from the Terudermis(R) , 1 x 1 cm sized,was removed. The Terudermis(R) was grafted on the exposed perichondrium in the experimental group. However, Terudermis(R) was not grafted in the control group. At 2 and 4 weeks after the surgery, the specimen was obtained and studied by histologic study. The results are as follows: 1. In control group at 2 weeks after surgery, the appearance of perichondrocytes and chondrocytes were not different from those of normal tissue. 2. In control group at 4 weeks after surgery, the extent of chondroblast differentiation and cartilage regeneration was insignificant compared to experimental group. 3. In experimental group at 2 weeks after surgery, we examined the active differentiation process of chondroblast beneath the perichondrium. The mean thickness of the neocartilage layer was 0.11+/-0.04 mm. 4. In experimental group at 4 weeks after surgery, there was an active regenerated new cartilage layer eneath the perichondrium, but the neocartilage layer was immature. The mean thickness of neocartilage layer was 0.33+/-0.10 mm. In conclusion, this study suggested that the grafted Terudermis(R) has an effect on chondrogenetic induction by activating the perichondrium.
Subject(s)
Rabbits , Cartilage , Chondrocytes , Chondrogenesis , Dermis , Ear , Membranes , Regeneration , Silicones , Skin , TransplantsABSTRACT
Widespread brain-derived neurotrophic factor (BDNF) mRNA expression has been detected in the region of catecholamine groups of the rat lower brainstem, while few BDNF-immunoreactive cells were found in this area. In the present study, a double-color immunofluorescence (IF) technique for BDNF and tyrosine hydroxylase (TH) after colchicine treatment was employed to evaluate the possible presence of BDNF immunoreactivity in the catecholamin-ergic cells of rat lower brainstem. Additionally, a double-color IF technique for BNDF and TH and in situ hybridiza-tion for BDNF mRNA were performed to see effects of hemorrhage on the expression of BDNF and its mRNA. We detected many new BDNF-immunoreactive cells in the A1, A2, A4, A6-A10 and C1-C3 cell groups and in the other lower brainstem nuclei where, without colchicine treatment, BDNF mRNA was expressed, but not BDNF immunoreactivity. In addition, the catecholaminergic neurons were found to express BDNF immunoreactivity with the co-existence being greatest, in percentage terms, in medullary catecholaminergic cell groups. Hypotensive hemorrhage, which activates medullary catecholaminergic neurons, induced the expression of BDNF immunoreactivity in catecholaminergic neurons (A1/C1 and C2) and increased the number of BDNF mRNA-containing neurons in the area. These results demonstrate that BDNF is regulated by activity in medullary catecholaminergic cell groups involved in central cardiovascular regulation.
Subject(s)
Animals , Rats , Brain Stem , Brain-Derived Neurotrophic Factor , Colchicine , Fluorescent Antibody Technique , Hemorrhage , In Situ Hybridization , Neurons , RNA, Messenger , Tyrosine 3-MonooxygenaseABSTRACT
We treated a total of 4 patients with midfacial hypoplasia, aged 12 to 19 years, using distraction osteogenesis between January 1998 and June 1999. In 3 patients with severe maxillary hypoplasia, we used rigid external distraction device developed by Dr. Polley. The distraction was performed from 5 days after Le Fort I osteotomy at a rate of 1 mm/day for 10 to 15 days. After distraction was completed, the device was left in place for another 6 weeks for bony consolidation. And then, an orthodontic face mask was used with elastic traction for 2 months. In one patient with partial hypoplasia of the midface, the osteotomized zygoma and a part of the maxilla was distracted selectively using rigid external distraction device a total of 15 mm. In the degree of SNA, mean value improved from 75.0(75.5, 75.0, 74.5) to 81.8(81.5, 83.0, 81.0) after 6 months later. In relapse rate, distracted length decreased from 10 mm to 6 mm, 15 mm to 8 mm, 13mm to 7 mm at 6 months later resulting in relapse rate of 44.3%. The follow-up period was from 7 to 26 months. Advantages of rigid external distraction device are highly effective technique of maxillary distraction, easy control of vector of distraction and no additional surgical procedure for removal of the device. In conclusion, the external distraction device is very useful for midface distraction.
Subject(s)
Humans , Follow-Up Studies , Masks , Maxilla , Osteogenesis, Distraction , Osteotomy , Recurrence , Traction , ZygomaABSTRACT
We treated 9 patients with hemifacial microsomia or facial asymmetry between April 1998 and November 1999. The age of patients ranged from 21 to 45 years(mean 24.6). Six were women, 3 were men. The follow up period was 6 to 24 months(mean 15.3 months). The operative procedure was based on the Ortiz Monasterio's simultaneous mandibuiar and maxillary distraction technique. Ortiz Monasterio only freed the pterygomaxillay junction of the affected side, leaving the nasal septum and pterygomaxillary junction of the unaffected side intact. They also used external corticotomy on mandible. We modified the original Ortiz Monasterio's method by using complete Le Fort I osteotomy with complete separation of both pterygomaxillary junction and mandibular osteotomy to avoid the resistance during the distraction. In one patient of scleroderma with severe atrophy of the mandible and soft tissue on the right face, a free scapular osteocutaneous flap was done. One month later, simultaneous distraction of the maxilla, the transferred scapula bone and the mandible was performed. Among the 9 patients, bidirectional distraction was done in one patient, and intraoral device was applied in 3 patients. After 5 days of latent period, distraction was performed at a rate of 1 mm per day. After 6 to 8 weeks of consolidation period, intermaxillary fixation and distraction device were removed. Preoperatively, the deviation of occlusal plane ranged from 8 to 13 (mean 10.5 ). The length of distraction ranged from 7 mm to 20 mm(mean 13 mm). In 2 patients, there were radiologic evidences of relapse 6 months after distraction, but no significant change of facial appearance was found. Seven patients have maintained stable preoperative occlusion of of teeth as previous conditions and preoperative lateral open bite was improved postoperatively in 2 patients. Postoperative occlusal plane ranged from 0 to 1.
Subject(s)
Adult , Female , Humans , Male , Atrophy , Dental Occlusion , Facial Asymmetry , Follow-Up Studies , Goldenhar Syndrome , Mandible , Mandibular Osteotomy , Maxilla , Nasal Septum , Open Bite , Osteotomy , Recurrence , Scapula , Surgical Procedures, Operative , ToothABSTRACT
The face is a variable part of the body and characterized not only by each part but also by the interrelation of the parts. Because facial image is very complex, it is very difficult to standardize the facial profile. However, it is necessary to establish the facial anthropometry in the infant. We perforrned an anthropometric study of the linear length of soft tissue in 13 places of the nasolabial area using a spreading caliper and ruler for the purpose of establishing normal interrelationship of regional profile in newborns and 2, 4, 6 month old babies. Each age group was composed of 40 normal babies. Followings are our results in the order of newborn, 2, 4, 6 months old. Widths of columella were 3.2 mm, 3.5 mm, 3.7 mm, 3.8 mm, respectively. Heights of columella were 4.7 mm, 4.9 mm, 5.2 mm, 5.3 mm. Distances between medial alar bases were 13.7 mm, 14.4 mm, 17.4 mm, 17.6 mm. Lengths from alar to tip of Cupid's bow were 9.5 mm, 10.0 mm, 10.5 mm, 10.6 mm. Lengths from columella base to tip of Cupid's bow were 8.4 mm, 9.9 mm, 10.2 mm, 10.5 mm. Lengths from columella base to center of Cupid's bow were 8.3 mm, 9.5 mm, 9.8 mm, 9.9 mm. Lengths of one limb of Cupid's bow were 2.7 mm, 3.1 mm, 3.4 mm, 3.5 mm. Lengths from tip of Cupid's bow to commissure were 13.4 mm, 14.7 mm, 16.4 mm, 16.9 mm. Intercommissural distances were 26.8 mm, 30.3 mm, 30.8 mm, 32.5 mm. Widths of philtral column at columella base were 3.1 mm, 3.6 mm, 3.7 mm, 4.0 mm. Distances between philtral columns(mid portion) were 3.7 mm, 4.6 mm, 4.6 mm, 4.6 mm. Heights of nasal tip protrusion were 8.7 mm, 11.0 mm, 11.7 mm, 12.1mm. Widths of nose were 20.7 mm, 23.7 mm, 25.3 mm, 25.9 mm. As the treatment of cleft lip should be performed within 6 months of age, the exact data for the face are very important, especially in the nasolabial area. We expect our data to be useful as a guideline for the management of cleft lip patient under one year old.