ABSTRACT
Separation, prolifration of stem cells and repairing of injured parts using these cells. This was a Lab-Experimental study. We used 6 male albino rabbits. At first, under general anesthesia 5x5 cm2 full thickness skin from one of the rabbits separated, washed using 70% alcohol and inserted in cold HBSS. Then it was cut into 4-6 mm pieces, washed again and incubated in Tripsin -EDTA 0.1% for 30 minutes at 37C. The cell suspention was then centrifuged and resuspended in DMEM culture. After 10 days the cells separated from the bed using Tripsin method. In experimental group the wounds were sprayed with 2 ml cultured keratinocytes cells and bandaged with vazeline. In control group just the skin was removed and the wounds healed withought cell spray. At the end of 4th week all rats were sacrificed, the repaired regions separated and studied with H and E and tricrom mason staining. In cell studies, the colonies of stem cells were visible using special staining. In Histological studies the epiderm of repaired wounds with cells were normal but the keratoid layers were thiner than normal skin. No sweat gland was observed. Other findings were: shorter finger, nodes, regular collagen fiber in dermal layer and wider vesseles. This method of cell culture would repaire a wide region and obtain a normal skin in a little time
Subject(s)
Male , Animals, Laboratory , Stem Cells , Epithelium , Rabbits , Skin , Cell Culture Techniques , KeratinocytesABSTRACT
Carpal tunnel syndrome [CTS] is the most common entrapment neuropathy in all peripheral nervous system e of it is one of the most important causes of disability in patients. This study was designed to compare the efficacy of luontophoresis and medical treatment for idiopathic CTS. 60 consecutive patients with CTS confirmed by electromyography participated randomly in two experimental groups [drug therapy], and luontophoresis. lntervention in each group include 20 daily treatment luontophoresis [4ma, positive electrode, 15 mm/session] applied to the area over the carpal tunnel of the wrist region. Measurement were performed before and after treatment and included pain assessment, electromyo and neurography measurement [motor and sensory latency, motor and sensory nerve conduction velocity], grip strength. Improvement was significantly more pronounced in Iuntophoresis group than drug therapy group for variables, motor latency [P<0/000], sensory latency [P< 0/005], hand grip [P<0/000], and pain relief [P<0/000]. In clinical findings atrophy was not observed. Nocturnal pain and paresthesia were reduced 80% in luntophoresis group but 46/6% in control group. Results indicate both luntophoresis and drugs were effective, but luntophoresis was better than drugs. Further investigation is needed to investigate the effects of combination of these treatments in patients with mild to sever CTS
Subject(s)
Humans , Iontophoresis , Dexamethasone , Electromyography , Hand StrengthABSTRACT
Background: mustard gas [sulphur mustard], the most widely used chemical agent in the Iran-Iraq war, affects many organs including the skin, respiratory tracts. This study was performed to evaluate respiratory complication on victims
Materials and Methods: in a historical cohort study, Two hundred and four victims were referred from the Mostazafan and Janbazan Foundation of kermanshah with confirmed mustard gas poisoning 14-17 years after the exposure were studied. Patient compared with control group
Results: nearly all the victims complained of cough, dyspnea, and suffocation. Mortality was 75% [respiratory failure] and 25 %[renal failure] in victim group and was % 14/2 [Malignancy], %14/2 [heart failure], %28/5 [accident], %42/8 [aging] in control group. Median age of death in this group were 65/5 years and in another group were 70
Conclusion: from this study we are able to provide a suitable practical plan for workup and management of patients in this setting
ABSTRACT
Background: in man the immunosuppressive drug Cyclosporine A [Cs A] has been used successfully in organ transplantation and in the treatment of autoimmune disorders and exerts toxic effect on various parenchymal organs. This study was performed with the aim of testing whether Cyclosporine plus vitamin A would be reversed the effect of cyclosporine on rat's testis
Methods and Materials: 40 male Wistar rats, 2 months old were divided into four groups [10 in each group]. Experimental groups [A] were injected daily 40 mg/kg body weight of Cyclosporine and olive oil subcutaneously and in group [B] rats were injected daily 40 mg/kg body weight of Cyclosporine and olive oil plus vitamin A. In group [C] rats were injected only olive oil and in group [D] rats were normal and without any injection. Rats were kept under optimal hygienic condition, temperature 25 degreeC, relative humidity 40 to 45% and light provided for a 12-h day/12-h night cycle for 2 weeks. They were given water and rodent pellets. After 2 weeks rats were killed and testis were fixed in formalin 10% and were stained and studied with light microscopy
Results: distances between seminiferous tubules were increased. Accumulation of necrotic materials of the cells in the lumen of seminiferous, oligospermia, displacement of seminiferous tubules and changing the situation of sertoli nuclei were seen. All of changes were not seen in group [B]
Conclusions: we can use vitamin A as a protective in young patient against Cs side effects
ABSTRACT
Background: epithelial is an important tissue in our body. The human epithelium forms a barrier between the external and internal environment
Materials and methods: this study was a Lab-Experimental. We were used 4 male albino rabbits in this study. At first, 6×6 cm[2] full thickness skin separated of rabbit and cultured in DMEM and FCS 10%. Then 2×1 cm[2] skin separated of other rabbit and cut into small pieces and transplanted on the first skin's derm. After 2 weeks, small pieces covered the entire feeder layer and then new skin grafted on the donor rabbits and supported for 2 weeks. Then after, histological studies were done
Results: histological studies were shown epidermal layer were normal but the number of layers were lower than normal skin. Other findings were thin cornified layer, low skin appendages, absent of sweat gland, regular of collagen fiber in dermal layer and small rete ridge
Conclusions: with this method of cell culture, skin can expand 18 times and a normal skin was obtained
ABSTRACT
Background: Sulfur mustard [bis [2-chloroethyl] sulfide] is a strong alkylating agent with known mutagenic and suspected carcinogenic properties
Materials and methods: Eighty NMARI male rats, 3 months old, were divided into eight groups [10 in each groups], were injected with 2.5, 5 and 10 mg /kg sulfur mustard plus Tyrods buffer. Rats were kept under optimal hygienic condition, temperature 25[degree]C , relative humidity 40 to 45% and light provided for a 12-h day/12- h night cycle. They were given water and rodent pellets. After 2 and 8 weeks rats were killed. The samples were fixed in formaldehyde solution [%10], were stained with H and E and PAS and were studied with light microscope
Results: Increased blood cells in hepatic sinusoids, disappearance borders of liver lobules, irregulation of hepatic cord, apoptotic appearance of cells in lobule, and these signs were seen in 2/5 mg/kg [8 weeks], 5mg/kg [2 and 8 weeks] and 10 mg /kg [2 and 8 weeks] groups. Glycogen decreased in 5mg/kg [8 weeks] and 10 mg/kg [2 and 8 weeks] groups
Conclusions: All of changes were dependent on dosage and time duration
ABSTRACT
Sulfur mustard [bis [2-chloroethyl] sulfide] is a strong alkylating agent with known mutagenic and suspected carcinogenic properties. Eighty NMARI male rats, 3 months old, were divided into eight groups [10 in each groups], were injected with 2.5, 5 and 10 mg /kg sulfur mustard plus Tyrods buffer. Rats were kept under optimal hygienic condition, temperature 25 C, relative humidity 40 to 45% and light provided for a 12-h day/12-h night cycle. They were given water and rodent pellets. After 2 and 8 weeks rats were killed. The samples were fixed in formaldehyde solution [%10], were stained with H and E and PAS and were studied with light microscope. Increased blood cells in hepatic sinusoids, disappearance borders of liver lobules, irregulation of hepatic cord, apoptotic appearance of cells in lobule, and these signs were seen in 2/5 mg/kg [8 weeks], 5mg/kg [2 and 8 weeks] and 10 mg /kg [2 and 8 weeks] groups. Glycogen decreased in 5mg/kg [8 weeks] and 10 mg/kg [2 and 8 weeks] groups. All of changes were dependent on dosage and time duration.
Subject(s)
Male , Animals , Liver/pathology , Rats , Alkylating Agents , Carcinogenicity Tests , Mutagenesis/drug effects , BuffersABSTRACT
Background: Cyclosporine is an immunosuppressive drug used largely in organ graft medicine with serious effects on liver parenchyma. We experienced combination of cyclosporine and vitamin A for liver damage risk reduction in rats
Materials and Methods: We random allocated 40 rats in 4 study groups and inoculated 1. cyclosporine with olive oil subcutaneously; 2. intramuscular cyclosporine and vitamin A; and 3. subcutaneous absolute olive oil. The 4[th] rat group was not injected and it considered as normal control group. Inoculation was continued for two weeks and rats were kept in standard equal environmental condition. By the end of the injection period they all were dissected and 2 liver tissue samples were studied from each rat using H and E stain and light microscope. Tukey test was used to compare kupfer cells between study groups by considering an alpha level of 0.05 as significant
Results: Hepatocytes disarrangement, congestion and hyperemia of sinusoids, and apoptotic cell enhancement were outcomes of cyclosporine use in rats. There was also blood in kupfer cells and obscure portal space. In comparison with vitamin A addition cyclosporine group showed increasing of kupfer cells [P<0.001]
Conclusion: Adding Vitamin A to cyclosporine regimen can probably results in liver damage risk reduction
ABSTRACT
Background: Sulfur mustard [HD] [bis[2-chloroethyl] sulfide] is a strong alkylating agent with known mutagenic and suspected carcinogenic properties
Materials and Methods: 40 Albino-Nmari male rats, 3 months old , were divided into four groups [10 in each group] and injected with 5, 10 mg /kg sulfur mustard in tyrods buffer After 8 weeks rats were killed. The samples were fixed in formaldehyde solution [%10], stained with H and E and studied with light microscope
Results: Increased distance between seminiferous tubules. Accumulation of necrotic materials of the cells in the lumen of seminiferous, oligospermia, displacement of somniferous tubules and changing the situation of sertoli nuclei from the abdominal positioning to basal in both groups of sulfur mustard that compared with normal and tyrods groups
Conclusion: Presence of necrotic form of spermatocytes and necrotic cells with picnotic nuclei in both sulfur mustard groups but more in 10 mg/kg HD
ABSTRACT
Background: Cyclosporine is an immunosuppressive drug used largely in organ graft medicine with serious effects on liver parenchyma. We experienced combination of cyclosporine and vitamin A for liver damage risk reduction in rats
Materials and Methods: We random allocated 40 rats in 4 study groups and inoculated 1. Cyclosporine with olive oil subcutaneously; 2. intramuscular cyclosporine and vitamin A; and 3. subcutaneous absolute olive oil. The 4[th] rat group was not injected and it considered as normal control group. Inoculation was continued for two weeks and rats were kept in standard equal environmental condition. By the end of the injection period they all were dissected and 2 liver tissue samples were studied from each rat using H and E stain and light microscope. Tukey test was used to compare kupfer cells between study groups by considering an alpha level of 0.05 as significant
Results: Hepatocytes disarrangement, congestion and hyperemia of sinusoids, and apoptotic cell enhancement were outcomes of cyclosporine use in rats. There was also blood in kupfer cells and obscure portal space. In comparison with vitamin A addition cyclosporine group showed increasing of kupfer cells [P<0.001]
Conclusion: Adding Vitamin A to cyclosporine regimen can probably results in liver damage risk reduction
ABSTRACT
Background: Sulfur mustard [HD] [bis[2-chloroethyl] sulfide] is a strong alkylating agent with known mutagenic and suspected carcinogenic properties
Materials and Methods: 40 Albino-Nmari male rats, 3 months old, were divided into four groups [10 in each group] and injected with 5 , 10 mg /kg sulfur mustard in tyrods buffer .After 8 weeks rats were killed. The samples were fixed in formaldehyde solution [%10], stained with H and E and studied with light microscope
Results: Increased distance between seminiferous tubules. Accumulation of necrotic materials of the cells in the lumen of seminiferous, oligospermia, displacement of somniferous tubules and changing the situation of sertoli nuclei from the abdominal positioning to basal in both groups of sulfur mustard that compared with normal and tyrods groups
Conclusion: Presence of necrotic form of spermatocytes and necrotic cells with picnotic nuclei in both sulfur mustard groups but more in 10 mg/kg HD