ABSTRACT
Background: Adipose tissue represents an accessible source of mesenchymal stem cells [Adipose tissue derived mesenchymal stem cells-ADSCs]. ADSCs could be differentiated into various mesenchymal stem cells [e.g. chondrocytes, adipocytes, osteoblasts, and neuronal lineages]. Neurotrophic factors are diffusible polypeptides that have a critical role in survival, proliferation and differentiation of stem cells. This study aimed to evaluate the proliferative capacity and the expression of some neurotrophic factors such as brain-derived neurotrophic factor [BDNF], ciliary neurotrophic factor [CNTF], glial cell-derived neurotrophic factor [GDNF], neurotrophin-3 [NT-3], neurotrophin-4 [NT-4], nerve growth factor [NGF] and the expression of nestin in ADSCs
Materials and Methods: Rat ADSCs were isolated from the subcutaneous adipose tissue using mechanical and enzymatic digestion. These cells were cultured in alpha MEM supplemented with 10% fetal bovine serum. ADSCs of the third passage were evaluated by immunocytochemistry to detect the cell surface markers of CD90 and nestin. Reverse transcription-polymerase chain reaction [RT-PCR] was used to study the expression of the above-mentioned neurotrophic factors in the ADSCs. Moreover, adipogenic and chondrogenic differentiation of ADSCs were induced in-vitro
Results: The assessment of ADSCs identity and purity showed that 90% and 80% of the stem cells were positive for CD90 marker and nestin, respectively. According to RT-PCR results, the above-mentioned neurotrophic factors were expressed in these stem cells. Furthermore, a small number of cells were positive for cresyl violet staining
Conclusion: Adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for cell replacement therapy