ABSTRACT
Objective: To study the time-effect and dose-effect relationships of reducing uric acid of one complex extraction. Methods: Sixty male Kunming mice were randomly divided into blank group, model group, allopurinol group, and three groups of high, medium, and low complex extraction group. In the study of time-effect relationship, the mice were continuously treated with complex extraction for 2, 4, 7, 10, 13, 16, and 20 d, then the rats model was established by ip administration with oxonic acid potassium salt (20 mL/kg); In the study of dose-effect relationship, the mice were continuously treated with complex extraction for 7 d, then the rats model was established by ip administration with oxonic acid potassium salt (20 mL/kg). The mice urine, blood, and liver tissue were collected. Results: In the study of time-effect relationship, after 4 d of administration, the serum uric acid concentration was decreased obviously, and 7 d later, the low, medium, and high dose groups showed the significant activity of lowering uric acid, and the physiological and mental state of mice were very good in 20 d tests. Study on the relationship between dose and effect found that in low dose (100 mg/kg) showed good reduction of serum uric acid value activity (P < 0.05), and the high dose group (400 mg/kg) showed the most significant activity (P < 0.001). There was a certain dose-effect relationship. The high-dose group also showed significant inhibition of liver xanthine oxidase (XOD) activity (P < 0.05). Conclusion: The complex extraction has good reduction activity on uric acid in vivo, and shows certain dose-effect and time-effect relationships. The lowering effect on uric acid has relation with the inhibitory activity on xanthine oxidase.
ABSTRACT
sely related with bacterial translocation. Intestinal bacterial translocation (most commonly E. coli) might occur at early stage (2 h) after ab-dominal surgery. Postoperative SIRS and infection might bear a close relationship with bacterial translocation.