ABSTRACT
The tumor cell DNA content cell proliferation as determined by flowcytometry may provide an additional factor in the prognosis and therapy planning of colorectal carcinoma. This work tried to correlate these criteria to other histopathological prognostic factors in this common and morbid type of malignant tumor. Thirty cases of resected colorectal carcinoma and five cases of colonoscopy obtained carcinoma specimens were subjected to histopathological examination and DNA study by flowcytometry. The DNA index [DI] was not elevated with high tumor grade, but increased in the presence of perineural invasion. The proliferative index [PI] showed elevation in all cases and was highest with blood and lymph vessel invasion and in the presence of desmoplastic reaction. The S phase fraction [SpF] was also elevated. It is increased in adenocarcinoma more than in squamous cell carcinoma, with higher tumor grade and also with blood vessel invasion. It was concluded that the combined study of DI, PI and SpF of tumor cells in resected or endoscopy obtained specimens of colorectal carcinoma can be indicative of the tumor biologic behavior and help in therapy planning
Subject(s)
Humans , DNA, Neoplasm/analysis , Flow Cytometry , Carcinoma , DNAABSTRACT
The distribution of Schistosoma antigen and IgG was studied in mice infected by Egyptian strain S. mansoni before and after treatment with N. sativa. Daily oral dose of the ground seeds equivalent to 2 gm adult human dose was given to infected mice for four weeks. Histopathologic examination revealed decrease in the epithelioid cells and increase of lymphocytes and eosinophils within the bilharzial granuloma after treatment compared to untreated infected controls. There was moderate decrease in the Schistosomal antigen and increase in the IgG in the liver and intestine of treated animals as detected by immunoperoxidase technique. It was concluded that N. sativa improves the host immunity against S. mansoni