ABSTRACT
In this study, S3 antigen of T. spiralis and T. Pseudospiralis was given to the heterologous infection in albino mice. The recovered larvae were studied as regards their number, length, and steroscan examination. It was found that vaccination of mice with T. spiralis [S3] antigen induced reduction in T. pseudospiralis larval count by 66.43% and significant reduction in length. Using T. pseudospiralis [S3] antigen reduction of 74.77% in T. spiralis larval count was detected as well as significant reduction in length, scanning electron microscopic [SEM] examination revealed more or less similar finding in both species; loss of integrity of the cuticle, some of the larvae were attacked by granulocytes mainly eosinophils and few larvae appeared completely destructed with loss of normal architecture. A peculiar finding was noticed in case of T. pseudospiralis larvae in form of fine electron dense deposits giving the cuticular surface a mottling appearance
Subject(s)
Trichinella spiralis , Immunization , VaccinationABSTRACT
The surface morphology of adult and larvae of Trichinella spiralis after cyclosporin A treatment was studied by scanning electron microscopy [SEM] of fixed, dried and metal coated specimens. The earliest topographic changes revealed in adults after the drug treatment were in form of disorganization of the cuticle, especially between the hypodermal pores which appeared somewhat thickened and irregular. Other changes were in the form of bullae formation covering the whole width of the adult surface. Other specimens showed wrinkling of the outer layer that was seen later to peel off. In few worms, layer outer was damaged, releasing some of the internal organs. Smoothening of the cuticle with obliteration of the grooves between the cuticular ridges were observed as the earliest surface larval changes, ulcer formation which was deeply seated was seen in patchy areas in some specimens. The interaction between the parasite surface and the drug is of significant importance, as this can throw light on the possibilities of its mode of action
Subject(s)
Animals , Cyclosporins , Microscopy, ElectronABSTRACT
The antigenic fraction of a cell free homogenate of each of Trichinella spiralis and T. pseudospiralis were prepared to be used for induction of immunity against S. mansoni in albino mice. The results of the study showed that immunization with either of the two antigens induced reduction in worm load ranging from 80. 48% to 87, 86% in the different groups of the study. Reduction in tissue egg count was also reported: Liver egg count was reduced from 69. 47% to 100%. Intestinal egg count reduction ranged from 73. 83% to 99. 78%. There was also reduction in egg excretion as evidenced by diminution in stool egg count. Liver granulomas were less in number and size and showed accelerated reaction together with inhibition of fluorescence. These findings were more manifest in animals given T. spiralis than T. pseudospiralis antigen
Subject(s)
Animals, Laboratory , Immunization , TrichinellosisABSTRACT
During the last few years, antiparasitic effects has been related to the immunosuppressant cyclosporin A [CsA]. This work was planned to study the effect of this drug against different aspects of experimental trichinosis. The work included three experiments. The first experiment was to study the effect of [CsA] on infection when administered to mice at different durations i.e. post infection as well as before and on the same day of infection. It proved to be most effective when given on the same day of infection as evidenced by the significant reduction in adult count, length of female, larval count and index of reproductive capacity [RCI]. However, the effect though still significant, diminished when the drug was given pre- and post- infection. The second experiment was to study the effect of [CsA] on infectivity of the T. spiralis larvae treated either in vivo or in vitro. The results showed that the drug has marked effect in that part of the study as shown by the reduction in all parameters of assessment .The third experiment demonstrated the challenge that followed [CsA] treated primary compared to that of non treated one. Results showed significant reduction in all values. These results evoke ideas for further designs of other treatment and control stratigies for the disease