The use of a coagglutination test to presumptively identify Salmonella typhi in bone marrow-oxgall medium cultures from typhoid fever patients.

A study was conducted to test a coagglutination procedure for detection of Salmonella typhi in bone marrow cultures from suspected typhoid patients admitted to Friendship Hospital, Jakarta, Indonesia. The results of the coagglutination tests were compared to the results from standard cultural isolation and identification. Bone marrow aspirates (356) were cultured in oxgall medium and aliquots subcultured daily for 7 days while simultaneously testing for the presence of Salmonella group D and Vi antigens using coagglutination (COAG). S. typhi was isolated from 220 (62%) of the cultures and the D- and Vi-COAG tests were positive for those same cultures. The COAG test was also negative for 6 cultures containing S. paratyphi A. The COAG results were available within 10 minutes after 18 to 24 hours incubation of the primary cultures whereas the isolation and confirmed identification took 2 to 3 days longer. The COAG test is valuable as an aid to rapidly identify S. typhi in bone marrow-oxgall cultures.

A penicillinase test incorporated in the rapid carbohydrate fermentation method for rapid detection of beta-lactamase producing Neisseria gonorrhoeae.

A modification of the acidometric (phenol red) test for penicillinase producing N. gonorrhoeae was incorporated into the rapid fermentation method for rapid screening and identification of PPNG strains. Two hundred and twenty-four non-penicillinase-producing N. gonorrhoeae, 55 penicillinase-producing N. gonorrhoeae, 87 N. meningitidis and 89 N. lactamica were included in this study. Results of the modified test were comparable with the iodometric and penicillin disk diffusion susceptibility and were obtainable within 1 to 5 minutes.

Isolation of Salmonella typhi from standard whole-blood culture vs blood-clot cultures.

The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a 1:4 and 1:10 blood to medium ratio, respectively. Clot cultures in 10% oxgall (CLOX) gave a 57% positive isolation rate for S. typhi. A similar result was obtained from clot cultures in bile broth medium (CLBB). A total of 184 samples identified as positive for S. typhi were tested. There was no significant difference between the use of 10% oxgall or bile broth medium when used for clot culture. The whole blood culture systems still showed a significantly better rate of isolation than the clot culture methods.

Variation in the hemolytic activity of Leptospira interrogans serovar canicola.

Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium with hemin. The hemolysin produced in this culture system showed a greatly expanded spectrum of hemolysis as compared to previous reports of leptospiral hemolysin produced in a more traditional culture system containing serum.

Hemolysin production by Leptospira interrogans serovar canicola in a protein-free medium with hemin.

Leptospira interrogans serovar canicola strain moulton was grown to a high cell density in a protein-free medium. When hemin was added to this medium, hemolysin was produced. Hemolysin was not detected when other porphyrins or cytochrome C were substituted for hemin in the medium.

Cesarea. Qual a incidencia aceitavel? / Cesarean. What is the acceptable incidence?

Com o objetivo de encontrar uma incidencia de cesariana aceitavel, assunto tao discutido hoje, as cesareas e partos vaginais ocorridos num servico obstetrico foram observados, com 24,4% de cesareas, nao ocorrendo diferencas significativas entre os dois metodos, quanto a morbidade materma e morbi-motalidade perinatal. Recomenda-se manter a incidencia de cesareas em torno de 25%

Culdoscopia: metodo endoscopico em ginecologia. / Culdoscopy: endoscopic method in ginecology

E apresentada a experiencia dos autores relativa a 500 culdoscopias. A anestesia preferente foi a peridural, utilizada em 82% dos casos. O exame foi realizado com as pacientes na classica posicao genupeitoral, a qual permite exposicao da pelve vista em angulo de 90 gruas e dispensa a insuflacao de qualquer gas. Em nenhum caso se procedeu a sutura do orificio deixado pela perfuracao do trocater. As principais indicacoes foram esterilidade e dor pelvica, aguda ou cronica, de causa a esclarecer. Os resultados mostraram variada patologia. Em 26,4% dos casos, os achados foram normais. Em 3,8% das oportunidades, o exame foi incompleto ou inconclusivo.Houve seis complicacoes: perfuracoes extraperitoneais do reto, as quais nao deixaram sequelas. Conclui-se que a culdoscopia, apesar de pouco conhecida, nao deixa de ser meio pratico e recomendavel, para fins diagnosticos, em qualquer servico