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OBJECTIVE To provide reference for the subsequent landing of national medical insurance negotiated drugs （referred to as “national negotiated drugs”） at the provincial level. METHODS By reviewing the data publicly released by the official websites of National Healthcare Security Administration and the Healthcare Security Administration of Zhejiang Province， combined with policy documents， the descriptive analysis was conducted on the number of tertiary medical institutions， the actual allocation of national negotiated drugs， the availability rate of national negotiated drugs， the allocation rate of national negotiated drug varieties， and the allocation rate of medical institutions of various cities in Zhejiang province. The Spearman rank correlation test was used to analyze the correlation between the number of types of national negotiated drugs equipped in tertiary medical institutions in Zhejiang province and the per capita disposable income， the number of tertiary medical institutions equipped with national negotiated drugs， and the implementation time of disease diagnosis-related grouping （DRG） of various cities in Zhejiang province. RESULTS As of the first quarter of 2022， 135 tertiary medical institutions in Zhejiang province were equipped with a total of 261 types of national negotiated drugs， accounting for 94.91% of the 2021 edition of the National Negotiated Drugs Catalogue （275 types）. The allocation rates of Goserelin acetate sustained-release implant， Sacubitril valsartan sodium tablets， Alteplase for injection and other varieties were at high level， and the types of national negotiated drugs equipped were highly coincident with the top 10 causes of death with disease of urban and rural residents in Zhejiang province. The tertiary medical institutions in Hangzhou had the most types of national negotiated drugs， with 230 types， while Quzhou had the lowest， with only 34 types； allocation rate of national negotiated drugs in medical institutions of Zhoushan was the highest （100%）， while that of Lishui was the lowest （57.14%）. The types of national negotiated drugs equipped were positively correlated with per capita disposable income in various cities and the number of tertiary medical institutions equipped with national negotiated drugs （P＜0.01）， and there was no significant correlation with the length of implementation of DRG （P＞0.05）. CONCLUSIONS mail：lanyao@mails.tjmu.edu.cn The landing of national negotiated drugs in Zhejiang province is generally good， with a high rate of equipping tertiary medical institutions with national negotiated drugs and a high rate of equipping drug varieties. Therefore， it is recommended that the provincial implementation of national negotiated drugs should be multi-faceted， and policy-making departments should adopt a dual-channel of “unbundling” and “driving” to smooth the drug chain into hospitals. The health insurance sector should improve the “dual channel” management mechanism to share the pressure on hospitals to use drugs. At the same time， it should also improve the multi-level medical security system and raise the level of reimbursement of medical insurance for national negotiated drugs.
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Objective:To evaluate the effect of down-regulating lncRNA LINC00263 targeting miR-4458 on the proliferation, migration, invasion and radiosensitivity of breast cancer SK-BR-3 cells.Methods:The expression differences of LINC00263 in breast cancer tissues, adjacent tissues, normal breast epithelial cells and breast cancer cells were determined by qRT-PCR. Transfection of LINC00263 shRNA in breast cancer SK-BR-3 cells down-regulated the expression of LINC00263, and the cloning experiment was used to detect the radiosensitivity. Breast cancer SK-BR-3 cells were treated with 6 Gy irradiation. CCK-8 assay was employed to detect cell proliferation. Flow cytometry was adopted to detect cell apoptosis. Transwell chamber test was performed to detect cell migration and invasion. Western blot was used to detect the expression levels of C-Caspase-3 and C-Caspase-9, MMP-2 and MMP-9 proteins. Bioinformatics software predicted that LINC00263 and miR-4458 had complementary binding sites, and the luciferase reporter system was utilized determine the targeting relationship between LINC00263 and miR-4458. LINC00263 shRNA and miR-4458 inhibitor were co-transfected into breast cancer SK-BR-3 cells, and 6 Gy irradiation was given to detect the changes in cell proliferation, apoptosis, invasion and migration.Results:The expression level of LINC00263 in breast cancer tissues was higher than that in adjacent tissues. The expression level of LINC00263 in breast cancer cells was higher compared with that in normal breast epithelial cells. The radiosensitivity of breast cancer SK-BR-3 cells was increased after transfection of LINC00263 shRNA. Transfection of LINC00263 shRNA and radiation exerted a synergistic effect, jointly inhibited breast cancer cell proliferation, migration and invasion, promoted cell apoptosis, up-regulated the expression levels of C-Caspase-3 and C-Caspase-9 proteins in cells, and down-regulated those of MMP-2 and MMP-9 proteins. Down-regulation of LINC00263 targetedly up-regulated miR-4458 expression. miR-4458 inhibitor reversed the inhibitory effect of LINC00263 shRNA combined with radiation on the proliferation, migration, invasion and apoptosis promotion of breast cancer SK-BR-3 cells.Conclusion:Down-regulating lncRNA LINC00263 targeting miR-4458 inhibits the proliferation, migration and invasion of breast cancer SK-BR-3 cells, and improves cell radiosensitivity.
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Objective To compare the detection effects of Legionella pneumophila in water samples from public places by the Legiolert enzyme-substrate method and the conventional cultivation. Methods The Legiolert enzyme-substrate method and the conventional cultivation method were used to detect Legionella pneumophila in cooling water and shower water samples collected in public places. Isolated strains were verified and serotyped. Results A total of 68 samples were collected and tested. The positive rate of the conventional cultivation and the Legiolert enzyme-substrate method were 5.88%（4/68）and 35.29%（24/68）, respectively, with a significant difference （χ2=16.41，P2=16.41，P=0.000). A total of 25 strains of Legionella pneumophila were isolated，and the serum types were mainly LP1（14/25）. Conclusion The Legiolert enzyme-substrate method represente a higher detection rate of Legionella pneumophila in water samples from public places than the conventional cultivation.
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Objective To express Tp0751 laminin-binding adhesion of Treponema pallidum (T. pallidum) ,and assess the immunocompetence. Methods The Tp0751 ORF without upstream non-cod-ing region was ligated into the expression vector pET-28a( + ), and expressed in E. coli R2566. Its immuno-gen was analyzed by Western blot and ELISA. Results A fusion protein with molecular weight about 26×103 was attained after expression and purification. Western blot proved that the recombinant protein can specifically react with T. palliclum IgG positive sera. Specific humoral response were elicited after introducing recombinant protein in Zealand rabbit and the specific antibody titer was above 1:10 2400 detected by indi-rect ELISA. Conclusion The expressed recombinant protein showed excellent immunoeompetence, and the results lay the foundation for the research on its function to T. paUidum infection.
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Purpose:To study the role of postoperative radiotherapy in early stage uterine sarcomas. Methods:Retrospective analysis of the impact of radiotherapy on progress-free survival rate (PRS )rate, local recurrence rate (LRR) and overall survival (OS)in stage Ⅰ,Ⅱ uterine sarcomas. 34 cases were treated with surgery alone ,42 cases were treated with postoperative radiotherapy. Results:The PRS at 2 and 5 years for surgery were 38.2% and 26.5% ,69.0% and 57.1%for postopeative radiotherapy. The LRR at 2 and 5 years treated with surgery were 52.9% and 64.7%,26.2% and 28.6% with adjuvant irradiation. The OS at 2 and 5 years treated with surgery were 83.4%and 47.0%,83.3% and 69.0% with adjuvant radiation. Conclusions:Postoperative radiotherapy showed a marked increase in the three parameters studied (PRS? LRR?OS ) for I,II stage uterine sarcomas.
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Purpose:To study the adjuvant therapy in uterine sarcomas,to decrease local recurrence and metastases and improve survival.Methods:Analysis of the management of 113 cases of uterine sarccoma , retrospectively. 30 cases were treated weith surgery alone , 25 with adjuvance radiation , 34 with postoperative chemotherapy, 21 with surgery and chemo radiation.Results:The 2 year pelvic recurrence and metastases with surgery alone were 43.3 % and 30 0 %,32 0 % and 28 0 % with postoperative radiation,44.1 % and 20.6 % with postoperative chemotherapy, 23.8 % and 23.8 % with postoperative combination of chemo radiation.But there was no significant difference between surgery alone and postoperative adjuvant therapy.Conclusions:Postoperative radiotherapy can improve local control,adjuvant chemotherapy can decrease metastases,both can improve disease free survival,but can not improve overall survival for uterine sarcomas.
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Background and purpose:Several prior studies have evaluated that the overall incidence of cervical cancer has decreased in the past 30 years, while it seems that the age of mortality is younger, especially young women under 35 years old. The prognosis of cervical cancer in young women still needs to be determined. The purpose of this study was to determine the main clinicopathologic characteristics of cervical cancer in young women aged under 35 years and the influence of age on prognosis. Methods:A retrospective review was performed for 831 patients under 35 years old , 1 737 cases over 35 years old was used as a control group. Clinical and pathologic variables including clinical stage, pathological type, tumor size, lymph node metastases(LNM),depth of invasion(DI) and lymphovascular space involvement (LVSI)were analyzed using Peason chi-squowe test. The 5-year survival and 2-year recurrence rates were analyzed by Kaplan-Meier method.Results:The younger patients were characterized by a higher rate of early stage with 64% vs 37.8% in control group(P=0.000),non-squamous cell carcinoma were 14.90% in the study group and 6.80% in the control group(P=0.000),bulky lesion were 52.00% vs 30.44%(P=0.000), LNM were 33.46% vs 23.93% (P=0.000),deep stroma invasion(DSI) were 51.88% vs 39.94% (P=0.005),There was no significant difference in LVSI for two groups with 37.03% in the young group and 38.26% in the control group (P=0.663).The clinical stage and tumor size of young patients were related with LNM,DI and LVSI(P