ABSTRACT
Objective:To investigate the expressions of hypoxia-inducible factor-1α(HIF-1α)and heat shock protein 70(HSP70)in the placenta tissue of the patients with pregnancy-induced hypertension syndrome(PIH), and to elucidate the clinical significances of their expressions in the placenta tissue of the patients with PIH. Methods:Twenty-two cases of placenta tissue of the PIH patients(PIH group)and eighteen cases of placenta tissue of the normal pregnant women(control group)were selected.The expressions of HIF-1αand HSP70 were detected by immunohistochemical method.The differences of potive expressions rates of HIF-1α and HSP70 in placenta tissue of the PIH patients and the normal pregnant women and their relationships in placenta tissue of the PIH patients were analyzed.Results:The positive expression rate of HIF-1α in placenta tissue of the patients in PIH group(81.8%)was higher than that of the normal pregnant women in control group(38.9%)(χ2=7.785,P=0.005),and the positive expression rate of HSP70 in placenta tissue of the patients in PIH group(90.9%)was higher than that of the normal pregnant women in control group(55.6%)(χ2=6.599,P=0.010).Eighteen cases of placenta tissue with HIF-1α positive expression in the PIH patients had HSP70 positive expression(100.0%);among four cases of placenta tissue with HIF-1α negative expression,two cases had HSP70 negative expression (50.0%);their expressions in placenta tissue of the PIH patients had positive correlation(r=0.671,P=0.001). Conclusion:The expressions of HIF-1αand HSP70 in placenta tissue of the PIH patients are higher,and they have positive correlation.
ABSTRACT
Objective To study the seasonal change of antibodies levels in the serum of asthma patients and Der P1 concentration in the dust samples collected from their bedrooms. Methods 36 asthma patients who were allergic to mites were treated as the patients group,21 male and 15 female,aged 43 years of average age. 20 healthy individuals were treated as the control group,11 male and 9 female,with the same age as the patients group. In September and December in 2005,and Match and June in 2006,the dust samples in the bedrooms of patients and the venous blood were collected,the samples of the control were collected only in September. Der P1 concentrations in dust samples and the levels of total IgE,mite specific IgE (s-IgE),s-IgG1,s-IgG2,s-IgG3 and s-IgG4 were detected by ELISA assay. Results In autumn,the levels of total IgE,s-IgE,s-IgG1,s-IgG2,s-IgG4 and the Der P 1 concentrations in patients were significant higher compared with the control group (P