ABSTRACT
Male infertility is a distressingly common condition affecting about 1 in 20 of the male population. In a majority of the cases, the male partner produces sufficient numbers of spermatozoa to achieve fertilization but there are functional defects in these cells that prevent conception from occurring. In this study, the effects of Wi-Fi radiation on spermatogenesis, testes and testosterone of large white male rats were studied, and also hydro alcoholic extract of sea buckthorn fruit were used as a protective agent against the effects of Wi-Fi radiation, and the comparative study was done.For this study 60 white male laboratory rats weighing 200-250 g were selected then tested in good conditions of light and food. The mice were randomly divided in terms of Wi-Fi Radiation and received gavage Sea buckthorn into 6 out of groups of 10.It was found that in the groups that had received Wi-Fi radiation, waves did not affect sperm count, testes weight, testosterone and testicular tissue, but it caused a significant decrease in fertilized sperm motility, and sea buckthorn extract could work as a protective agent against the radiations. According to the results of this research, it can be the probability that Sea buckthorn extract, having antioxidant substance can prevent destructive effects of waves that cause free radicals and having vitamin E improves sperm quality
ABSTRACT
Glioblastoma Multiforme [GBM] or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. Several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications. Proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. In this study proteins were extracted from the tumor and normal brain tissues and then the protein purity was evaluated by Bradford test and spectrophotometry. In this study, proteins were separated by 2-Dimensional Gel [2DG] electrophoresis method and the spots were then analyzed and compared using statistical data and specific software. Protein clustering analysis was performed on the list of proteins deemed significantly altered in glioblastoma tumors [t-test and one-way ANOVA; P< 0.05]. The 2D gel showed totally 876 spots. We reported, 172 spots were exhibited differently in expression level [fold > 2] for glioblastoma. On each analytical 2D gel, an average of 876 spots was observed. In this study, 188 spots exhibited up regulation of expression level, whereas the remaining 232 spots were decreased in glioblastoma tumor relative to normal tissue. Results demonstrate that functional clustering [up and down regulated] and Principal Component Analysis [PCA] has considerable merits in aiding the interpretation of proteomic data. 2D gel electrophoresis is the core of proteomics which permitted the separation of thousands of proteins. High resolution 2DE can resolve up to 5,000 proteins simultaneously. Using cluster analysis, we can also form groups of related variables, similar to what is practiced in factor analysis
ABSTRACT
Dihydropyrimidinase Related Proteins [DRPs] have known homologous to the Collapsing Response Mediator Proteins [CRMPs]. The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding. The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel [2DG] electrophoresis method and then spots have analyzed and compared using statistical data and specific software [Progenesis Same Spots].Spots have identified by pH isoelectric, molecular weights and data banks. The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values. This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity