ABSTRACT
Aim: The aims of this study were to investigate antibiotic resistance pattern and molecular characterization of Salmonella Infantis strains, isolated from human sources in Tehran hospitals from 2008 to 2010
Background: Infection caused by Salmonella is one of the major public health problems. Despite the clinical importance of Salmonella enteric subsp. enteric serovar Infantis in humans, there is no information available about the common clones of Salmonella Infantis in clinical isolates in Iran
Methods: S. Infantis strains were identified by conventional microbiological and serological testing. The antimicrobial susceptibility of the S.Infantis isolates was determined using the disk diffusion method. The genetic relatedness and the dominant clones of S. Infantis strains were detected by the Multi Locus Sequence Typing [MLST] and pulsed-field gel electrophoresis [PFGE] techniques
Results: More than 80% of the S. Infantis isolates represented multidrug-resistant patterns. PFGE revealed high genetic similarity among S. Infantis strains. While, MLST indicated high-clonal similarity among strains, where all S. Infantis strains were assigned to the ST32 sequence type
Conclusion: This is the first study in Iran conducted to determine the sequence types of S. Infantis in clinical isolates using MLST. The genetically closed MDR S. Infantis clones were responsible for the apparent endemic occurrence of salmonellosis, caused by this Salmonella serovar, in Tehran
ABSTRACT
Background and Aims: Given the importance of aminoglycoside resistance in nosocomial and community infections caused by bacterial pathogenes such as Klebsiella pneumoniae [K. pneumoniae], the aim of this study was to determine the frequency of aac [6']- Ib and aac [3]- IIa, the genes encoding aminoglycoside modifying enzymes involved in aminoglycoside resistance
Material and Methods: A total of 100 K. pneumonia isolates were collected from hospitalized patients from April to September 2015 in Borujerd hospitals. Conventional microbiological tests were carried out to detect and confirm K. pneumonia isolates. Antibiotic susceptibility of isolates was detected by disk diffusion methods. The presence of the aac[6']-Ib and aac[3]-IIa genes which encode aminoglycoside modifying enzymes was determined by polymerase chain reaction
Results: Among 100 K. pneumonia isolates, 34% showed resistance to gentamicin and 21% to amikacin. Resistance to both gentamicin and amikacin was detected in 18% of the isolates. Multi-resistance phenotypes were detected in 71% of the isolates. The aac [3]-IIa and aac[6']-Ib genes were found in 71% [n=24] and 5.8% [n=2] of aminoglycoside resistant isolates, respectively. Simultaneous carriage of aac [3]-IIa and aac[6']-Ib was detected in 64% [n=22] of the aminoglycoside resistant isolates
Conclusions: The results of this study showed the presence of aac [3]-IIa genes in more than 70% of the aminoglycosides resistant K. pneumoniae strains; this may be due to the transmission of this gene through mobile genetic elements that create a high risk of rapid spread of these genes in hospitals
ABSTRACT
Aim: In This study focused on the detection of dominant clones and genetic relationship of Shigella spp. isolated from children with diarrhea in the main pediatric hospital in Ahvaz by multi-locus sequence typing [MLST] technique
Background: Shigellosis is considered as one of the problematic bacterial infections for public health in the world. Khuzestan province in the Southwestern part of Iran is a known endemic area for infections due to Shigella. There are limited molecular epidemiological data for Shigella spp. in this area
Methods: A total of 50 Shigella spp. were isolated from January-June 2015 based on conventional microbiology and serology tests. The Sequence types [ST] of Shigella isolates which are characterized by Enterobacterial repetitive intergenic consensus [ERIC-PCR] were detected by MLST technique
Results: Among 50 Shigella isolates, a total of 31[62%], 16[32%] and 3 [6%] of Shigella isolates were identified as S. flexneri, S.sonneii, and S.boydii, respectively. Two different sequence types [ST152 and ST245] were identified in Shigella isolates. ST152 was detected in S.sonnei and ST245 in S. flexneri and S. boydii isolates
Conclusion: Based on MLST data, the stable and genetically linked Shigella clones are the cause of Shigella infections in children in Southwestern Iran
ABSTRACT
Shigellosis is one of the important gastrointestinal bacterial infections, particularly among children of developing countries such as Iran. Antibiotic susceptibility pattern and genetic typing for epidemiological purposes are of significant issues in Shigella infectious control
The aim of this study was to investigate the antibiotic susceptibility and genetic relationship among Shigella strains isolated from children with shigellosis at paediatric hospital in Ahvaz, south west of Iran. This study included all Shigella strains isolated from paediatric patients with diarrhea admitted to Abuzar pediatric hospitals in Ahvaz, during January-June 2015. Shigella isolates were identified using standard microbiological and serological methods. Shigella spp strains also were studied by antimicrobial susceptibility testing and Enterobacterial Repetitive Intergenic Consensus [ERIC] - PCR analysis. Total of 50 Shigella strains were isolated from children with dysentery diarrhea. In total, 31 [62%] were identified as Shigella flexneri, 16[32%] and 3 [6%] were Shigella sonnei and Shigella boydii respectively. High level resistance were detected against ampicillin, trimethoprim-sulfamethoxazole and cephalotine. All isolates were sensitive to ceftriaxone, imipenem gentamicin and amikacin
The results of ERIC-PCR data analysis showed 11 different types of Shigella with four closely-related patterns. S. flexneri was the predominant serogroup of Shigella spp. in children in the referral pediatric hospital in Ahvaz
Ampicillin and trimethoprim-sulfamethoxazole is no longer recommended for shigellosis empirical treatment and should be replaced by other antibiotics such as ceftriaxone or ciprofloxcacin. Diverse but genetically close strains of shigella were responsible for shigellosis in paediatric patients in Ahvaz, south west of Iran?