ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of repeated administration of Zhuhong ointment on renal antioxidant capability of ulcerous skin in rats, in order to further discuss the mechanism of mercury contained in Zhuhong ointment on the antioxidant capability of kidney in skin ulcer rats.</p><p><b>METHOD</b>Eighty SD rats were randomly divided into eight groups: Zhuhong ointment A, B, C, D, E (1.219, 0.609, 0.305, 0.152, 0.76 g x kg(-1)) groups, the vaseline group, the ulcer model group and the impairment control group. The levels of NAG and RBP of toxicity for early kidney tubular injury and T-AOC, SOD, GSH-PX and GSH in kidney were determined after consecutive administration for 14 days.</p><p><b>RESULT</b>Compared with ulcer model group, the levels of RBP in groups A, B, C and D increased, while the levels of NAG increased only in the group A. The level of T-AOC increased in groups A, B and C. The level of T-SOD increased in the group E, while it dropped down greatly in the group A. The level of GSH-PX increased in groups A, B and C. The content of GSH increased in every dose groups.</p><p><b>CONCLUSION</b>Antioxidant capacity in rats can be increased in a reasonable dose of Zhuhong ointment, but some antioxidant activity can be notably inhibited by with the increase of dose.</p>
Subject(s)
Animals , Male , Rats , Acetylglucosaminidase , Urine , Antioxidants , Metabolism , Disease Models, Animal , Dose-Response Relationship, Drug , Drugs, Chinese Herbal , Pharmacology , Toxicity , Glutathione , Metabolism , Glutathione Peroxidase , Metabolism , Kidney Tubules , Wounds and Injuries , Metabolism , Mercury , Metabolism , Ointments , Random Allocation , Rats, Sprague-Dawley , Retinol-Binding Proteins , Urine , Skin Ulcer , Metabolism , Microbiology , Specific Pathogen-Free Organisms , Staphylococcal Skin Infections , Metabolism , Superoxide Dismutase , Metabolism , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Zhuhong ointment on accumulation in the body of mercury and the pathological morphology changes of kidney, via the measurement of related indicators of the skin-impaired model rat.</p><p><b>METHOD</b>Eighty-eight SD rats were randomly divided into the impairment control group, and high-, middle-, low-dose Zhuhong ointment groups. Each group was treated by corresponding methods for 4 weeks, and recovering for 4 weeks. Urinary potein (PRO), pH, Beta N-acetyl aminoglycosidase enzymes (NAG) and beta2-microglobulin (beta2-MG) contents in urine were taken as monitoring indexes, blood urea nitrogen (BUN) and serum creatinine (SCr) in blood and the levels of mercury in urine, blood and kidney were tested, and the pathological morphology changes of kidney were observed.</p><p><b>RESULT</b>After treatment for 4 weeks, compared with impairment control group, the levels of mercury in urine, blood and kidney in every dose group increased significantly (P < 0.01). And the relation exists between toxicity and dose on Zhuhong ointment. After recovery for 4 weeks, the levels of mercury in urine and blood in every dose group restore normal, while the level of mercury in kidney in high- dose group still increased (P < 0.01). The level of NAG increased only in high-dose group. There was no significant difference in NAG contents between Zhuhong ointment groups and the impairment control group (P < 0.05).</p><p><b>CONCLUSION</b>Excess using Zhuhong ointment repeatedly may lead to accumulation of mercury and pathological morphology changes of kidney. So the levels of mercury in the body and related indicators of renal functions should be tested in clinical when long-term using Zhuhong ointment.</p>
Subject(s)
Animals , Female , Male , Rats , Acetylglucosaminidase , Urine , Blood Urea Nitrogen , Creatinine , Blood , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Toxicity , Hydrogen-Ion Concentration , Kidney , Metabolism , Pathology , Mercury , Blood , Metabolism , Urine , Ointments , Random Allocation , Rats, Sprague-Dawley , Retinol-Binding Proteins , Urine , Skin , Wounds and Injuries , Time Factors , beta 2-Microglobulin , UrineABSTRACT
The art of warm disease of TCM not only has abundant clinical experience,but also has a profound theoretical thinking,and it starts with its unique concept category and logical system,and riches and enriches,updates and develops on the basic of this,and goes along the road of independent development,has a distinct characteristics.This paper preliminarily studies on wei qi ying and xue of the art of warm disease of TCM from meaning,development of history,nature and action,et al.And it provides references of ideas and methodologies for the development and innovation of the art of warm disease of TCM.