ABSTRACT
Aims: This research explored the effect of ABA treatment before short-term night sub-low temperature on the sugar composition and contents, and provided theoretical basis for temperature management in tomato protected cultivation. Study Design: The whole tomato was treated by spraying with 50mmol/L and 100mmol/L ABA respectively before short-tern night sub-low temperature. Spraying distilled water was as the control. The lower temperature treatment was as the lower temperature control group. Place and Duration of Study: Biological Science and Technology College, between February 2013 and March 2014. Methodology: The sugar composition and contents were determined using High Performance Liquid Chromatography (HPLC). Results: The pectinic tissues, pericarp and dissepiments after sub-low temperature treatment had high contents of three soluble sugars at the early fruit development stage. The fructose and glucose contents were reduced by the 50mmol/L ABA treatment under condition of lower temperature. At the fruit mature stage, contents of three soluble sugars in the pericarp and dissepiments after sub-low temperature treatment had less than those in control group and had higher accumulation than the other two groups by 50mmol/L ABA treatment, but the soluble sugars contents were reduced by ABA treatment in the pectinic tissues. Conclusion: 50mmol/L ABA was the optimum concentration on improving the ability of resisting sub-low temperature in tomato fruits at the early development stage, improving the tomato fruit quality at the fruit mature stage, promoting transfer of soluble sugars to structure matter or storage matter. However, the effects of ABA on sugar accumulation were different in the pectinic tissues or pericarp and dissepiments.
ABSTRACT
Aims: Find a suitable method for the protein extraction from flower buds of Solanum lycopersicum. Study Design: Compare some kinds of protein extraction methods and find the best one among them suitable to tomato flower buds. Place and Duration of Study: Biological Science and Technology College, between June 2010 and July 2011. Methodology: The proteins for electrophoresis were extracted using different methods, such as trichloroacetic acid /acetone (TCA/acetone), Sodium dodecyl sulfate (SDS), Trissaturated phenol (Tris-Phen), Phenol/SDS and Direct lysis method. After silver staining, different patterns of protein spots were observed in the gels. Results: Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less protein productivity though more spots by Tris-Phen extractions, and more protein productivity and better apart effect by TCA/acetone. The 2-DE image background was the clear and the protein spots were the most by TCA/acetone method. Conclusion: TCA/acetone method is much more suitable as extraction method for protein two-dimensional electrophoresis of tomato flower buds.