ABSTRACT
Objective: To investigate the effects of procyanidin B2 on the expression of inflammatory mediators in human periodontal ligament cells (hPDLCs) induced by Porphyromonas gingivalis lipopolysaccharide (LPS). Methods: hPDLCs were cultured using tissue explant method in vitro. The effect of procyanidin B2 on the cell viability of hPDLCs was detected by MTT assay. hPDLCs were stimulated by P. gingivalis LPS after treatment with procyanidin B2 for 1 h. The expressions of IL-1β, IL-6, and IL-8 mRNA and proteins were detected by real-time PCR and ELISA assay. Reactive oxygen species (ROS) in the cytoplasm was observed under fluorescence microscope. Nitric oxide (NO) in the supernatant was detected by Griess assay. Results: 100.00 µg/mL procyanidin B2 could enhance the cell viability of hPDLCs. Procyanidin B2 could inhibit the expressions of IL-1β, IL-6, and IL-8 mRNA and proteins in hPDLCs. It could also downregulate ROS and NO in hPDLCs induced by P. gingivalis LPS. Conclusion: Procyanidin B2 can play an anti-inflammatory role by inhibiting inflammatory mediators in hPDLCs induced by P. gingivalis LPS.