ABSTRACT
Objective: To investigate the expression pattern of tropomyosin 2(TPM2) in aorta of patients with aortic dissection and explore its clinical implication. Methods: Thirteen cases with acute type A aortic dissection(TAAD) diagnosed by transabdominal aortic angiography from 2015 in Tongji Hospital were included. During the operation, the aortic wall tissues of these patients were collected. Ten patients with heart transplantation were selected as control group, and normal aortic wall tissues were taken. The hematoxylin-eosin (HE) and Verhoeff's Van Gieson (EVG) staining were performed to observe the morphological changes of aorta. The mRNA expression level of TPM2 was measured by real-time fluorescent quantitative-PCR, and the protein levels of TPM2 were detected by Western blot and immunohistochemical staining. Image The J software was used to collect the optical density values of each point on the image, obtain the integrated optical density(IOD) value, and calculate the average density(%, IOD/area of the target distribution area). Results: HE and EVG staining revealed medial degeneration and broken elastic fiber in aorta of TAAD patients. The mRNA expression levels of TPM2 were significantly upregulated in aorta of TAAD patients as compared to the control group (P<0.05), so as the TPM2 protein expression levels ((9.73±1.20)% vs. (0.11±0.04)%, P<0.05). And TPM2 was mainly expressed in cytoplasm. Conclusion: The increased expression of TPM2 in TAAD patients hints that TPM2 might be involved in the pathogenesis of aortic dissection.
Subject(s)
Humans , Aortic Dissection/genetics , Aorta , Aortic Aneurysm, Thoracic/genetics , Gene Expression , RNA, Messenger , Tropomyosin/metabolismABSTRACT
<p><b>OBJECTIVE</b>To evaluate the safety and efficacy of needlescopic thoracic sympathectomy for palmar hyperhidrosis.</p><p><b>METHODS</b>From March 2004 to April 2005, 62 patients, including 23 men and 39 women with a mean age of 23 years (ranged from 12 to 53 years), underwent bilateral needlescopic thoracic sympathectomy. Among all the patients 8 cases had accompanied axillary hyperhidrosis. The degree of palmar sweating was moderate in 16 cases and severe in 46 cases. The sympathetic chain on the body of the second and third ribs in all patients was cauterized and divided; the chain on the fourth rib in those with axillary hyperhidrosis was also severed. Intraoperative changes in palmar temperature and blood flow were recorded.</p><p><b>RESULTS</b>Sympathectomies were successful, and dry limbs were immediately achieved in all patients after surgery. There were no mortality or life-threatening complication, however 1 patient developed moderate pneumothorax which resolved soon after chest drainage. After all procedures, palmar blood perfusion increased significantly and mean palmar temperature elevated by 2.4 degrees C. The mean operative duration was 65 min, and the mean postoperative hospital stay was 1.2 days. No recurrence of palmar hyperhidrosis occurred after a mean follow-up of 6.3 months (ranged from 1 to 13 months). Compensatory sweating was found in 26 patients, but the symptoms were mostly tolerable and required no further treatment.</p><p><b>CONCLUSION</b>Needlescopic thoracic sympathectomy is a safe and effective technique for palmar hyperhidrosis, which is less invasive than conventional video-assisted thoracic surgery.</p>
Subject(s)
Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Follow-Up Studies , Hand , Hyperhidrosis , General Surgery , Sympathectomy , Methods , Thoracic Surgery, Video-Assisted , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To improve the technique of prominent malar complex reduction.</p><p><b>METHODS</b>The improvements of the operation procedure included double-oblique osteotomy of the prominent malar complex, accessorial tiny preauricular incision and the way that the complex was moved upward, inward or posteriorly. The zygomatic body was fixed with microplate screw or steelwire. The jugal soft tissue was lifted to prevent facial slack. The method was used in 17 cases from June 2000 to April 2004.</p><p><b>RESULTS</b>Postoperative follow up for 4 approximately 24 months showed satisfactory result in all the cases.</p><p><b>CONCLUSIONS</b>This modified method resolved some problems in reduction malarplasty through an intraoral approach.</p>
Subject(s)
Adult , Female , Humans , Male , Young Adult , Osteotomy , Methods , Plastic Surgery Procedures , Methods , Treatment Outcome , Zygoma , General SurgeryABSTRACT
<p><b>OBJECTIVE</b>To investigate the pathogeny diagnosis, therapy and some possible method for prevention of intercurrent infection, after the operation of augmentation mammaplasty.</p><p><b>METHODS</b>Comparing and analyzing the clinical representation and therapy of the seventeen intercurrent infecting case that we had, after the operation of the augmentation mammaplasty. The patients were treated by operating with different approaches.</p><p><b>RESULTS</b>After the operation, all the infected patients recovered in a short while without back to surgery.</p><p><b>CONCLUSIONS</b>The infection after augmentation mammaplasty will get an effective controlling result if take a timely operative therapy according to the position and character of the intercurrent infection. But some galactophore will shrink after the cure of infection.</p>