ABSTRACT
The Arabidopsis thaliana tonoplast Na+ /H+ antiporter gene, AtNHX1, was transferred into buckwheat by Agrobacterium-mediated method. Transgenic buckwheat plants were regenerated and selected on MS basal medium supplemented with 2.0mg/L 6-BA, 1.0mg/L KT, 0.lmg/L IAA, 50mg/L kanamycin and 500mg/L carbenicillin. 426 seedlings from 36 resistant calli originated from 864 explants (transformed about at 4.17 percentage) exhibited resistance to kanamycin. The transformants were confirmed by PCR, Southern blotting, RT-PCR and Northern blotting analysis. After stress treatment for 6 weeks with 200mmol/L NaCl, transgenic plants survived, while wild-type plants did not. After 3 days of stress treatment through different concentrations of NaCl, transgenic plants accumulated higher concentration of Na+ and proline than the control plants. However, the K+ concentration of transgenic plants declined in comparison with the control plants. Moreover, the rutin content of the roots, stems and leaves of transgenic buckwheat increased than those of the control plants. These results showed that it could be possible to improve the salt-tolerance of crops with genetic technology.
Subject(s)
Adaptation, Physiological , Genetics , Physiology , Arabidopsis Proteins , Genetics , Physiology , Blotting, Northern , Blotting, Southern , Cation Transport Proteins , Genetics , Physiology , Fagopyrum , Genetics , Metabolism , Physiology , Plant Roots , Genetics , Metabolism , Physiology , Plant Stems , Genetics , Metabolism , Physiology , Plants, Genetically Modified , Genetics , Metabolism , Physiology , Potassium , Metabolism , Proline , Metabolism , Regeneration , Reverse Transcriptase Polymerase Chain Reaction , Rutin , Metabolism , Sodium , Metabolism , Sodium Chloride , Pharmacology , Sodium-Hydrogen Exchangers , Genetics , Physiology , Transformation, GeneticABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of vertebral manipulation (VM) therapy on vertebro-basilar artery (VBA) blood flow in patients with cervical spondylosis of vertebral artery type (CS-VAT) by transcranial Doppler (TCD) ultrasonic examination.</p><p><b>METHODS</b>One hundred and fifty patients with CS-VAT were randomized into the VM group (n = 100) and the acupuncture group (n = 50), and treated for ten times as one therapeutic course. Changes of the contraction peak, the end-diastolic and average blood flow velocity of VBA before and after treatment in the two groups were observed and compared by TCD.</p><p><b>RESULTS</b>Vp, Vd, Vm of LVA, RVA and BA in the two groups after treatment were all lowered, showing significant difference, excepting Vp of VBA in the acupuncture group, when compared with before treatment (P< 0.05 or P <0.01). Comparison between the two groups after treatment showed significant difference in Vp and Vm of LVA, Vp, Vd and Vm of RVA, Vp and Vm of VBA respectively (P<0.05, P <0.01).</p><p><b>CONCLUSION</b>VM therapy in treating patients with CS-VAT shows therapeutic effect superior to VA therapy, which could significantly improve VBA blood flow.</p>