ABSTRACT
This study aimed to provide a scientific basis for the application of the mycorrhizal planting technology of Dendrobium officinale by investigating the effects of mycorrhizal planting on the fingerprints of D. officinale and the content of six chemical components. Seventeen samples of D. officinale under mycorrhizal and conventional planting were collected from four regions, such as Jinhua of Zhejiang. The HPLC fingerprints were established to evaluate the similarity of the samples. The content of six chemical components of the samples was determined by HPLC. There were 15 common peaks in the fingerprints, and five of them were identified by marker compounds, which were naringenin, 4,4'-dihydroxy-3,5-dimethoxybibenzyl, 3,4'-dihydroxy-5-methoxybibenzyl, 3',4-dihydroxy-3,5'-dimethoxybibenzyl(gigantol), and 3,4-dihydroxy-4',5-dimethoxybibenzyl(DDB-2). The similarities of the fingerprints of mycorrhizal and conventional planting samples and the control fingerprint were in the ranges of 0.733-0.936 and 0.834-0.942, respectively. The influences of mycorrhizal planting on fingerprints were related to planting regions, the germplasm of D. officianle, and the amount of fungal agent. The content of six chemical components in the samples varied greatly, and the content of DDB-2 was the highest, ranging from 69.83 to 488.47 μg·g~(-1). The mycorrhizal planting samples from Chongming of Shanghai and Taizhou of Jiangsu showed an increase in the content of 5-6 components, while samples from Zhangzhou of Fujian and Jinhua of Zhejiang showed an increase in the content of 1-2 components. The results showed that mycorrhizal planting technology did not change the chemical profile of small molecular chemical components of D. officinale, but affected the content of chemical components such as bibenzyls, which has a good application prospect.
Subject(s)
Dendrobium/chemistry , Mycorrhizae , China , Chromatography, High Pressure LiquidABSTRACT
Dendrobium officinale is a member of the family Orchidaceae. The dried stem of D. officinale is used as a valuable traditional Chinese medicine, known as Dendrobii Officamlis Caulis (called TiepiShihu in Chinese). According to Chinese Pharmacopoeia, Dendrobii Officamlis Caulis has effects of tonifying stomach, promoting fluid, nourishing Yin and clearing heat. At present, the planting area of D. officinale is over 100 000 Mu (over 6 670 hm2) and the annual output of its fresh stem is in excess of 10 000 tons. Good variety is the guarantee of herbal medicine's quality, while germplasm resource is the base for breeding excellent variety. In this paper, we summed the characteristics of present main varieties of D. officinale and reviewed the progress on germplasm resources and genetics and breeding of the plant, in order to provide a scientific basis for the further research.
ABSTRACT
OBJECTIVE: To provide scientific basis for variety evaluation and selection of Dendrobium officinale. METHODS: The agronomic traits of D. officinale were measured. The chemical compositions of D. officinale stem were analyzed. The dynamic changes of plant biomass and chemical composition content during mycorrhizal cultivation with M2 (Mycena sp.)were observed. RESULTS: There were significant differences between D. officinale phenotype A and B in stem length, internode length, leaf width and leaf shape index (P<0.05), and in the content of polysaccharide and ethanol soluble extract (P<0.05). Moreover, the contents of 4, 4'-dihydroxy-3, 5-dimethoxybibenzyl and 3, 4'-dihydroxy-5-methoxybibenzyl were always very different between phenotype A and B during plant growth. After 12 months of growth, the stem dry weight and plant dry weight of M-A group were significantly higher than those of CK-A group(P<0.01), increasing by 37.6% and 37.3%, respectively. The contents of polysaccharide and six marker compounds of M-A group were increased by 36.8% and 5%-50% than those in CK-A group, respectively. After 12 months of growth, the contents of polysaccharide and six marker compounds of M-B group were increased by 16.7% and 25%-170% than those in CK-B group, respectively. CONCLUSION: The bibenzyl content and the response ability to mycorrhizal fungi M2 of D. officinale phenotype A and B are different.
ABSTRACT
<p><b>BACKGROUND</b>Functional neuroimaging study has opened an avenue for exploring the pathophysiology of cluster headache (CH). The aim of our study was to assess the changes in brain activity in CH patients by the regional homogeneity method using resting-state functional magnetic resonance imaging technique.</p><p><b>METHODS</b>The functional magnetic resonance imaging scans were obtained for 12 male CH patients with spontaneous right-sided headache attacks during "in attack" and "out of attack" periods and 12 age- and sex-matched normal controls. The data were analyzed to detect the altered brain activity by the regional homogeneity method using statistical parametric mapping software.</p><p><b>RESULTS</b>Altered regional homogeneity was detected in the anterior cingulate cortex, the posterior cingulate cortex, the prefrontal cortex, insular cortex, and other brain regions involved in pain processing and modulation among different groups.</p><p><b>CONCLUSION</b>It is referred that these brain regions with altered regional homogeneity might be related to the pain processing and modulation of CH.</p>