ABSTRACT
Aim To investigate the effect of pyropheophorbide-a methyl ester-mediated photodynamic therapy (MPPa-PDT) on migration in human breast cancer MCF-7 cells as well as its possible molecular mechanism. Methods MCF-7 cells were randomly divided into control group, MPPa group, light group and MP- Pa-PDT group. Cells were co-cultured with MPPa at different concenlrations(0, 0. 5, 1,2,4 jimol • L"1) and exposed to light of different energy intensities, 0. 9, 1. 8, 2. 7, 3. 6 J/cm2). Cell viability was measured by CCK-8; production of ROS was detected by DCFH-DA staining; cell migration was mesured by Tr- answell migration assay and wound healing assay; the expressions of E-cadherin, PTEN, Akt, p-Akt were measured by Western blot. Results Compared with control group, MPPa group and light group, MPPa- PDT could significantly inhibit cell viability of MCF-7 cells in an energy-dependent manner. DCFH-DA staining suggested ROS in MPPa-PDT group was higher than that in the other three control groups; Transwell assay and wound healing assay showed cell migration in MPPa-PDT group was significantly inhibited; Western blot analysis indicated that the expression of E-eadhcr- in, PIXN in MPPa-PDT group increased, while p-Akt decreased. Conclusions MPPa-PDT inhibits MCF-7 cells' migration, and PTEN/AKT signal pathway may be involved in the process.
ABSTRACT
<p><b>OBJECTIVE</b>To observe the preventive protection of Kangdu Bufei Decoction (KBD) on acute severe respiratory syndrome (SARS) in medical personnel of Hong Kong at the epidemic period.</p><p><b>METHODS</b>At the epidemic period of SARS in Hong Kong, there were 2,601 medical staffs administered with KBD distributed by the Center. It was confirmed that 1,063 persons had taken it for successive two weeks according to the request and returned the effective questionnaire, they were regarded as the TCM group. The control group was consisted of 15,374 subjects who didn't take Chinese herbal medicine. Contents of the questionnaire including the condition of testee in terms of quality of life (QOL), changes of influenza-like symptoms and Warm disease symptoms. Serum immunological examination had been conducted in 37 of the persons in the TCM group.</p><p><b>RESULTS</b>No one in the TCM group got SARS infection while in the control group, 64 (0.4%) had got. Self-control before and after treatment examination showed that significant improvement appeared in the TCM in aspects of influenza-like and Warm disease symptoms and QOL. The results of serum immunological examination showed that after administration of KBD, the immunity of organism was improved and elevated.</p><p><b>CONCLUSION</b>KBD could prevent the occurrence of SARS, it is possibly realized through improving symptoms, elevating QOL and enhancing immunity of organism.</p>