ABSTRACT
Objective To investigate the effect of siomycin A-induced forkhead box protein M1(FoxM1) down-regulation on the malignant behaviors (proliferation, apoptosis, and invasive ability) of laryngeal carcinoma cells. Methods The experiment was performed with Hep-2cells of the logarithmic growth phase. The experimental groups were treated with different doses of siomycin A, and the control group was treated without siomycin A CCK-8 test was used to detect the viability of Hep-2 cells after treatment for 24, 48, and 72 h. CFSE staining was used to examine the proliferation ability of Hep-2 cells after treatment for 24 and 48 h. Annexin V_FITC/PI dual staining was used to observe the siomycin A-induced apoptosis and Transwell test was used to examine the invasion ability of Hep-2 cells after treatment for 48 h. The expressions of FoxM1, Ki-67 and cleaved caspase-3 protein inHep-2 cells were detected by Western blotting analysis. The supernatant levels of matrix metalloproteinase (MMP)-2 and MMP-9were examined by ELISA assay. Results (1) Treatment with siomycin A significantly decreased the expression of FoxM1 protein compared with the control group (P<0. 05). CCK-8 test found that siomycin A of different concentrations suppressed the viability of Hep-2 cells in a time- and dose-dependent manner. (2) Siomycin A at 1. 3 and 1. 5 µmol/L significantly suppressed the proliferation of Hep-2 cells in a dose-dependent manner (P<0. 05), accompanied by down-regulated expression of Ki-67. (3) Siomycin A at 1. 3 and 1. 5 µmol/L also induced greater apoptosis of Hep-2 cells (10. 6%, 27. 9%) in a dose-dependent manner compared with control group (4. 91%, P<0. 05), accompanied by up-regulated cleaved caspase-3 expression. (4) Siomycin A at 1.3 and 1. 5 µmol/L suppressed the invasive ability of Hep-2 cells in a dose-dependent manner compared with control group (P < 0. 05), accompanied by down-regulated expression of MMP-2 and MMP-9. Conclusion Down-regulation of FoxM1 by siomycin A can inhibit the proliferation and invasive ability of laryngeal carcinoma cells.
ABSTRACT
Objective To investigate the expression of forkhead box Ml (FoxMl) transcription factor and cyclooxygenase- 2 (COX-2) in nasopharyngeal carcinoma (NPC) tissues, and to discuss the related clinicopathological implications. Methods Immunohistochemistry method wasused to examine the expression of FoxM1 and COX-2 in80 NPC tissues and 40 normal nasopharyngeal mucosa tissues. The relationship of FoxM1and COX-2expression with the clinicopathological parameters of NPC was analyzed; we also analyzed the correlation between the FoxM1 and COX-2 expression. Results FoxM1 and COX-2 expressions were significantly higher in NPC tissues compared to normal nasopharyngeal mucosa tissues (FoxM1: 68. 8% [55/ 80] vs 2. 5% [1/40]; COX-2: 62. 5% [50/80] vs 5. 0% [2/40]; P<0. 05). The expression of FoxM1 was significantly associated with lymph node metastasis (rs =0. 252, P<0. 05) and clinical stage of NPC (rs = 0. 230, P<0. 05), and expression of COX-2 was also significantly associated with lymph node metastasis (r = 0. 287, P<0. 05) and clinical stage of NPC (r = 0. 239, P<0. 05). Furthermore, a positive correlation was observed between FoxM1 and COX-2 expression (rs = 0. 631, P< 0. 05), indicating a direct or indirect interaction between them. Conclusion Increased expression of FoxM1 and COX-2 in NPC tissues might be associated with the development and progression of NPC.
ABSTRACT
Objective: To evaluate the role of HPV16E6,cyclin D1 ,and human telomerase transcriptase (hTERT) in the development and progression of nasopharyngeal carcinoma ( NPC ) and to discuss the clinical significance. Methods: Immunohistochemistry was used to detect the expression of HPV16E6, cyclin D1, and hTERT in paraffin-embedded nasopharyngeal carcinoma tissues and nasopharyngeal chronic inflammation tissues. The relationship between their expression with the clinicopathological features of NPC was analyzed; the influence of their expression on prognoses of patients was also analyzed. Results: The positive rates of HPV16E6,cyclin D1 ,and hTERT in NPC tissues were 62. 5%(35/56) ,50. 0%(28/56) , and 67. 9%(38/56) ,respectively, which were significantly higher than those in the inflammation tissues (P0. 05). HPV16E6 expression was positively correlated with cyclin D1(r=0. 480,P<0. 001) and hTERT (r=0. 494,P<0. 001)in NPC tissues. The mean survival period and median survival period in HPV16E6,cyclin D1 and hTERT positive patients were lower than those in the negative ones (P<0. 05). Conclusion: High expression of HPV16E6,cyclin D1 ,and hTERT might be involved in the development and progression of NPC. HPV16E6 may interact with cyclin D1 and hTERT, contributing to the development of NPC. Examination of the three agents may help to predict the prognoses of NPC.