ABSTRACT
Bronchial asthma is a chronic airway allergy disease involving the imbalance of Th1/Th2. Th2 shift is one of the primary causes of asthma. Recent studies have found that T cell immunoglobulin and mucin domain molecule 1 (TIM-1) play important roles in T cell activation and Th1/Th2 differentiation in asthma, which has been intensively reported in recent researches. This article reviews the characteristics of TIM-1 gene and its role in the pathogenesis of bronchial asthma.
ABSTRACT
Objective To investigate the effect of T cell Ig and mucin 1 (TIM-1) on MUC5AC and Th2 cytokine expression in the airway of asthmatic mice, so as to understand the mechanism of airway mucus hypersecretion. Methods Thirty healthy female C57BL/6 mice were randomly divided into control,asthmatic and TIM-1 mAb treated groups, with 10 mice in each group. The proportion of TIM-1 positive cells in peripheral blood mononuclear cells (PBMCs), MUC5AC mRNA expression in the airway, levels of IL-13, IL-4 and IL-5 in bronchoalveolar lavage fluid (BALF), and the number and volume of airway mucous cells were examined in the three groups. Results The proportions of PBMCs TIM-1 positive cells in asthmaticGl. 20%) and TIM-1 mAb treated (5. 11%) mice were significantly higher than that in the control group (0. 64%, P<0. 05), and that in TIM-1 mAb treated mice was significantly lower than that in the asthmatic mice (P<0. 05). (2) MUC5AC mRNA expression in the airway mucous cells in the asthmatic (17. 3 + 1. 4) and TIM-1 mAb treated (5. 6 + 0. 3) mice were significantly higher than that in control mice (P<0. 05). The BALF levels of IL-13 (ng/ml), IL-4 (pg/ml), and IL-5 (pg/ml) were 16. 80 ±0. 63, 614. 72 + 117. 39, and 1 681. 13 + 613.55 in the asthmatic, and were 5. 70 ± 0. 64, 325. 78 ± 86. 54, and 513. 42 + 86. 87 in TIM-1 mAb treated mice, respectively, which were all significantly higher than those in the control mice ([1. 09 + 0. 25] ng/ml for IL-13, [17. 56 + 3. 01] pg/ml for IL-4, and [30. 78 + 9. 67] pg/ml for IL-5, P<0. 05). And all the above parameters in the TIM-1 mAb treated mice were significantly lower than those in the asthmatic mice (P
ABSTRACT
Bronchial asthma is a chronic airway allergy disease involving the imbalance of Th1/Th2. Th2 shift is one of the primary causes of asthma. Recent studies have found that T cell immunoglobulin and mucin domain molecule 1 (TIM-1) play important roles in T cell activation and Th1/Th2 differentiation in asthma, which has been intensively reported in recent researches. This article reviews the characteristics of TIM-1 gene and its role in the pathogenesis of bronchial asthma.