ABSTRACT
OBJECTIVE@#To explore the clinical efficacy of acupuncture combined with granule for nerve-root type cervical spondylosis and its effects on serum interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and hemorheological indexes.@*METHODS@#A total of 114 patients with nerve-root type cervical spondylosis were randomly divided into an observation group and a control group, 57 cases in each group. The patients in both groups were treated with traction. The patients in the control group were treated with oral administration of granule, 4 g each time, 3 times a day, while based on the treatment of control group, the patients in the observation group were treated with acupuncture at Dazhui (GV 14), Tianzhu (BL 10), Houxi (SI 3), cervical Jiaji (EX-B 2), Quchi (LI 11), Hegu (LI 4) and Waiguan (TE 5), once a day. Both groups were treated for 4 weeks. The simplified McGill pain questionnaire (MPQ), neck disability index (NDI), numbness score, levels of IL-6, TNF-α, IL-1β in serum and hemorheological indexes were observed before and after treatment, and the clinical efficacy was compared between the two groups.@*RESULTS@#The total effective rate was 91.2% (52/57) in the observation group, which was higher than 71.9% (41/57) in the control group (<0.05). Compared before treatment, the scores of MPQ, NDI and numbness in the two groups were reduced after treatment (<0.05). After treatment, the scores of MPQ, NDI and numbness in the observation group were lower than those in the control group (<0.05). After treatment, the serum levels of IL-6, TNF-α and IL-1β in the two groups were reduced (<0.05), and those in the observation group were lower than the control group (<0.05). After treatment, the plasma viscosity, fibrinogen, low shear rate of whole blood viscosity and high shear rate of whole blood viscosity in the two groups were lower than before treatment (<0.05), and those in the observation group were lower than the control group (<0.05).@*CONCLUSION@#Acupuncture combined with granule have significant clinical efficacy for nerve-root type cervical spondylosis, which could reduce the serum levels of IL-6, TNF-α and IL-1β and improve hemorheology.
Subject(s)
Humans , Acupuncture Therapy , Interleukin-1beta , Interleukin-6 , Spondylosis , Therapeutics , Tumor Necrosis Factor-alphaABSTRACT
BACKGROUND: Using the rapid growth period of the chorioallantoic membrane from the 8th day to the 12th day, vascular growth at bone defect end and vascular implantation of a bone graft substitute into the human body can be simulated.OBJECTIVE: To load hydroxyapatite into a chick embryo chorioallantoic membrane model, and to establish an implanted revascularization model.METHODS: Twelve rosette eggs were incubated under the same suitable conditions for 8 days. After windowing, the eggs were randomly divided into two groups: experimental group was implanted with hydroxyapatite material to establish the model of chick embryo chorioallantoic membrane carrying hydroxyapatite; control group was implanted with filter paper material, sealed and placed into a constant temperature and humidity box to continue to hatch. At the 12th day of incubation, length of new vessels and length of new vessels per unit area in the two groups were observed, and the angiogenesis on the hydroxyapatite and filter paper was observed.RESULTS AND CONCLUSION: The mean length of new vessels and length of new vessels per unit area were 24.031 mm and 0.242 mm/mm2 in the experimental group, and 23.561 mm and 0.212 mm/mm2 in the control group, respectively. There were no significant differences between two groups. Additionally, vascular tissues were obviously observed on the hydroxyapatite samples in the experimental group. These experimental results show that the establishment of the model of chick embryo chorioallantoic membrane carrying hydroxyapatite provides a simple and accurate in vitro animal model for studies on ideal bone graft materials and angiogenesis. The hydroxyapatite has no effect on the angiogenesis on the chorioallantoic membrane.
ABSTRACT
OBJECTIVE@#To discuss the influencing factors of using late postmortem phenomena to estimate PMI and to provide experience for an accurate estimation.@*METHODS@#Forty-nine corpses of late postmortem were collected in Shaoxing City, Zhuji area from 2004 to 2011. The related factors were analyzed including season, scene, estimated PMI, exact PMI, cause of death and main factors effected PMI, etc.@*RESULTS@#Of all 49 cases, 20 corpses were outdoor, 11 were indoor and 18 were in water. Thirty-seven cases were successful to estimate PMI and 12 cases were unsuccessful. The main factors affected PMI were infection, poisoning, human destruction and high-pressure electric shock, etc.@*CONCLUSION@#In general, PMI can be correctly estimated by late postmortem phenomenon. When the cases included infection, poisoning and human destruction, we should estimate PMI with the comprehensive analysis.