Effect of oral motor intervention in improving brain function development in preterm infants: a randomized controlled trial / 中国当代儿科杂志

OBJECTIVE@#To study the effect of oral motor intervention (OMI) on brain function development in preterm infants.@*METHODS@#A total of 112 preterm infants were stratified into small-gestational-age (30-31@*RESULTS@#On day 7 of OMI, the small-gestational-age intervention group had lower upper bounds of voltage and bandwidth and a higher aEEG score than the small-gestational-age control group (@*CONCLUSIONS@#OMI can promote the maturation of aEEG background activities, improve neurobehavioral manifestations, and accelerate brain function development in preterm infants.

Expressions and prognostic significance of PTEN and PD-1 protein in patients with classical Hodgkin's lymphoma / 中华血液学杂志

Objective: To elucidate the expression levels of key immune biomarkers, phosphate and tension homology deleted on chromosome ten (PTEN) and programmed cell death protein1(PD-1),of different immune tolerance pathway in classic Hodgkin's lymphoma (CHL) to further determine their clinical role and prognostic significance. Methods: The clinical features and prognostic factors of 56 CHL patients, who were admitted to the TianJin Medical University Cancer Institute from February 2003 to August 2013, were retrospectively analyzed. PTEN and PD-1 protein expression levels were analyzed by immunohistochemistry, Epstein-Barr virus encoded RNA (EBER) was performed by in situ hybridization assay. Correlations between the expression of biomarkers and clinicopathologic parameters were examined and survival analyses were performed. Results: This cohort of 56 CHL patients included 34 males and 22 females with a median age of 25 years (ranged from 7 to 71 years). In a univariate analysis, age≥45, IPS score >2, EBER positive, high expression of PTEN protein conferred inferior 5-year OS and 5-year PFS; In a multivariate model, age≥45, IPS score >2, EBER positive, high expression of PTEN protein were identified as the independent adverse prognostic factors for CHL. Conclusions: This study suggested for the first time that PTEN was independent prognostic immune biomarkers in CHL, which provided the novel therapeutic strategy of immune therapy for CHL.

An analysis on impact of different parameters for radiation dosage of enhanced CT of chest / 中国医学装备

Objective: To explore the diagnostic dosage of enhanced computed tomography (CT) of chest and its influence factor through analyzed the data of radiation dosage of enhanced CT of chest. Methods: The scanning data of 297 patients who were implemented scan on 4 different CT devices were compared by using retrospective analysis. And they were divided into A, B, C and D group as different device. The differences of scan sequence among different devices were compared, and volume computed tomography dose index(CTDIvol), dose length product(DLP) and effective dose(ED) were recorded and analyzed. Results: The CTDIvols of 4 groups were (12.18±4.13), (8.86±3.18), (10.70±5.18) and 13.59mGy, respectively, and the differences among them were significant (F=38.58, P<0.05). And the DLPs of the 4 groups were (433.54±145.18), (311.62±112.75), (368.04±181.22) and (475.75±34.25)mGy·cm, and the differences among these groups were significant (F=36.33, P<0.05). Besides, the EDs of the 4 groups were (6.07±2.03), (4.36±1.58), (5.15±2.54) and (6.66±0.48) mSv, and the differences among these groups also were significant (F=36.32, P<0.05). And the comparison between any two means of radiation dosage were significant. Conclusion: The exposure dose of patients who received enhanced CT of chest was correlative with the type of CT device and acquisition parameters. And the effective dosage of patients under automatic tube current module was lower than that of fixed tube current module. And the modulation technique of current of ray tube as angle can efficiently reduce the effective dose. In clinical practice, patients' radiation dosage can be reduced by optimizing the scanning parameters.

Resveratrol increases sirtuin 1 expression in peripheral blood mononuclear cells of premature infants and inhibits the oxidative stress induced by hyperoxia in vivo / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To explore the effect of resveratrol on the levels of sirtuin 1 (SIRT1) and reactive oxygen species (ROS) in peripheral blood mononuclear cells (PBMCs) of premature infants exposed to hyperoxia.</p><p><b>METHODS</b>Peripheral blood and isolated PBMCs from premature infants (gestational age<32 weeks) without oxygen supplement were collected and were randomly assigned into four groups: control, air+resveratrol, hyperoxia, and hyperoxia+resveratrol. The PBMCs were cultured in vitro for 48 hours, then the ROS content in PBMCs was measured by laser scanning confocal microscopy. Malondialdehyde (MDA) content in the medium was measured by the whole spectrum spectrophotometer. SIRT1 positioning was assessed by immunofluorescence. SIRT1 expression levels in PBMCs were measured by Western bolt.</p><p><b>RESULTS</b>Compared with the control group, the level of SIRT1 in the air+resveratrol group increased significantly (P<0.05). The levels of ROS and MDA and the SIRT1 transposition rate in the hyperoxia group increased significantly, while the expression level of SIRT1 decreased significantly compared with the control group (P<0.05). The levels of ROS and MDA and the SIRT1 transposition rate decreased significantly (P<0.05), and the expression level of SIRT1 increased significantly in the hyperoxia+resveratrol group (P<0.05).</p><p><b>CONCLUSIONS</b>Resveratrol can increase SIRT1 expression in PBMCs and inhibit SIRT1 shuttle from nucleus to cytoplasm in order to increase the ability of antioxidative stress in premature infants exposed to hyperoxia, thereby reducing the oxidative stress injury in premature infants.</p>

Imaging features of nonepithelial tumors of the bladder / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To summarize the imaging features of nonepithelial tumors of the bladder.</p><p><b>METHODS</b>The Imaging findings in 20 surgically treated patients with pathologically proved nonepithelial tumors of the bladder were retrospectively analyzed. The tumors included leiomyoma (n = 9), pheochromocytoma (n = 6), leiomyosarcoma (n = 2), rhabdomyosarcoma (n = 1), carcinosarcoma (n = 1), inflammatory myofibroblastoma (n = 1).</p><p><b>RESULTS</b>The leiomyomas were round or ellipse in shape with a sharp border and homogeneous density, and showed a low signal intensity on T1WI and T2WI in 1/1 case; slight enhancement on CT after contrast enhancement in 6/7 cases; and a poor blood supply on color Doppler ultrasonography in 3/4 cases. The pheochromocytoma had a round or oval shape and clear border, and slightly lobulated in 4/6 cases, homogeneous density/echo/signal in 5/6 cases, calcification in 1 case, low signal intensity on T1WI and high signal intensity on T2WI in 1/1 case, moderate or marked enhancement on CT and MRI in 4/5 cases, and strong blood supply on color Doppler ultrasonography in 3/4 cases. The inflammatory myofibroblastoma showed the same imaging features as the pheochromocytomas. Other malignant tumors showed an irregular configuration, with a poorly defined border, heterogeneous density/echo/signal and moderate to strong enhancement on CT.</p><p><b>CONCLUSION</b>Most leiomyomas and pheochromocytomas of the bladder show some typical imaging features on CT, MRI and ultrasound, which are helpful in making correct diagnosis and treatment plan preoperatively. Other malignant nonepithelial bladder tumors do not show special imaging characteristics and can only be diagnosed qualitatively.</p>