ABSTRACT
Objective: To study the influence of morphine on the expression of synaptophysin (SYN) and synapse structure in mice hippocampus, so as to provide pathological evidence for studying the development and treatment of chronic morphine intoxication, addiction and abstinence symptoms of morphine. Methods: Twenty mice were evenly randomized into control group and experiment group. Mice in control group were injected with normal saline (0.1 ml daily) and those in experimental group were injected with morphine (0.1 ml, 1 mg daily). Thirty days later the mice in 2 groups were killed and their brain tissues were harvested and made into slices, stained with immunohistochemical techniques (SP) and photographed under the light microscope. The images were analyzed with the image analytical system and the data were statistically analyzed. Results: In the control group, positive staining of SYN was found in the entorhinal area, subiculum, stratum plextiforme, polymorphic layer of gyrus dentatus, stratum oriens, and stratum radiatum of hippocampus; weak positive staining of SYN was noticed in the stratum lacunosum-moleculare of hippocampus; positive staining of SYN was also found the membrane of pyramidal cells and granule cells, with the mean gray scale value of the hippocampal structure being 132.84 ± 8.67. Positively stained neurons was also found in the entorhinal area and the subiculum, with a intensity of (7.80 ± 1.03)/mm2. In the experiment group, the subiculum and polymorphic layers of gyrus dentatus were positively stained for SYN; the entorhinal area, stratum oriens, stratum radiatum and stratum lacunosum-moleculare of hippocampus were strongly positive of SYN; the membrane of pyramidal cells and granule cells were also strongly positive of SYN, with the mean gray scale value of the hippocampal structure being 116.27 ± 5.70. Strongly stained neurons were also found in the entorhinal area and the subiculum, with the intensity being (11.90 ± 1.45)/mm2. The number of SYN positive neurons and the intensity of SYN in the experimental group were higher than those in the control group (P<0.01). The hippocampal strucure looked like ground glass, with slight staining and many pyknotic nuclei and bubbles. Conclusion: Morphine can promote the SYN expression and apoptosis of neurons in the hippocampus, and therefore affect the structure and the function of the synapses in it.
ABSTRACT
Objective: To study the influence of morphine on the expression of nestin in the ependymal epithelia, central gray and hippocampal formations in mice. Methods: Twenty health mice were evenly randomized into control group and experiment group. Mice in the control group were injected with normal saline (0.1 ml daily) and those in the experimental group were injected with morphine (0.1 ml, 1 mg daily). Thirty days later, the mice brain samples were harvested and made into paraffin sections. Immumohistochemical ABC technique was used to observe the expression of nestin under light microscope. The images were analyzed with the image analytical system. Results: In the control group, the ependymal epithelia, the central gray, the periventricular gray substances and the hippocampal formations had weak expression of the nestin, with a mean gray scale of 150.98±13.31; there were 5 kinds of nestin-positive cells: (1) the basal cells of ependymal epithelium, (2) cells distributed in the periventricular gray substance and the deep lamella of central gray, (3) cells distributed in the superficial lamella of central gray, the subiculum, the parahippocampal gyrus and the cortex in II, III layers of the entorhinal area, (4) cells frequently seen in the tectum of rnidbrain and the subiculum, and (5) cells distributed in the tectum of midbrain, the hippocampus, gyrus dentatus, parahippocampal gyrus and the cortex in V layer of the entorhinal area; the density of nestin in the subiculum and entorlimal area was (7.20 ± 1.23) mm2. In the experiment group, the ependymal epithelia, the central gray, the periventricular gray substances and the hippocampal formations had positive expression of the nestin, with the mean gray scale being 133.03 ± 22.28; the density of the above-mentioned 5 kinds of cells increased; the density of nestin in the subiculum and entorhinal area was (10.50±1.43) mm2. The mean value of gray scale and nestin-positive neurons were significantly different between the 2 groups (P<0.05). The cellular proliferation was seen in the ependymal epithelia. The second kind of cells appeared in the superficial lamella of central gray, parahippocampal gyrus and the cortex in I, II and III layers of the entorhinal area. The third kind of cells increased in the hippocampal pyrarnidal layer and the fourth kind of cells increased in the tectum of midbrain and the subiculum. Conclusion: Morphine can promote nestin expression in the ependymocyte, the central gray, the periventricular gray substance and the hippocampal formation; it can also promote the proliferation, differentiation and migration of the ependymocytes and neurocytes.
ABSTRACT
Objective:To study the influence of morphine on the expression of nestin in the ependymal epithelia,central gray and hippocampal formations in mice.Methods:Twenty health mice were evenly randomized into control group and experiment group.Mice in the control group were injected with normal saline(0.1 ml daily)and those in the experimental group were injected with morphine (0.1 ml,1 mg daily).Thirty days later,the mice brain samples were harvested and made into paraffin sections.Immumohistochemical ABC technique was used to observe the expression of nestin under light microscope.The images were analyzed with the image analytical system.Results:In the control group,the ependymal epithelia,the central gray,the periventricular gray substances and the hippocampal formations had weak expression of the nestin,with a mean gray scale of 150.98?13.31;there were 5 kinds of nestin-positive cells:(1) the basal cells of ependymal epithelium,(2)cells distributed in the periventricular gray substance and the deep lamella of central gray, (3)cells distributed in the superficial lamella of central gray,the subiculum,the parahippocampal gyrus and the cortex inⅡ,Ⅲlayers of the entorhinal area,(4)cells frequently seen in the rectum of midbrain and the subiculum,and(5)cells distributed in the tectum of midhrain,the hippocampus,gyrus dentatus,parahippocampal gyrus and the cortex in V layer of the entorhinal area;the density of nestin in the subiculum and entorhinal area was(7.20?1.23)mm~2.In the experiment group,the ependymal epithelia,the central gray,the periventricular gray substances and the hippocampal formations had positive expression of the nestin,with the mean gray scale being 133.03?22.28;the density of the above-mentioned 5 kinds of cells increased;the density of nestin in the subiculum and entorhinal area was(10.50?1.43)mm~2.The mean value of gray scale and nestin-positive neurons were significantly different between the 2 groups (P
ABSTRACT
Objective:To study the influence of morphine on the expression of synaptophysin(SYN)and synapse structure in mice hippocampus,so as to provide pathological evidence for studying the development and treatment of chronic morphine intoxication, addiction and abstinence symptoms of morphine.Methods:Twenty mice were evenly randomized into control group and experiment group.Mice in control group were injected with normal saline(0.1 ml daily)and those in experimental group were injected with morphine(0.1 ml,1 mg daily).Thirty days later the mice in 2 groups were killed and their brain tissues were harvested and made into slices,stained with immunohistochemical techniques(SP)and photographed under the light microscope.The images were analyzed with the image analytical system and the data were statistically analyzed.Results:In the control group,positive staining of SYN was found in the entorhinal area,subiculum,stratum plextiforme,polymorphic layer of gyrus dentatus,stratum oriens,and stratum radiatum of hippocampus;weak positive staining of SYN was noticed in the stratum lacunosum-moleculare of hippocampus;positive staining of SYN was also found the membrane of pyramidal cells and granule cells,with the mean gray scale value of the hippocampal structure being 132.84?8.67.Positively stained neurons was also found in the entorhinal area and the subiculum,with a intensity of(7.80?1.03)/ mm~2.In the experiment group,the suhiculum and polymorphic layers of gyrus dentatus were positively stained for SYN;the entorhinal area,stratum oriens,stratum radiatum and stratum lacunosum-moleculare of hippocampus were strongly positive of SYN;the membrane of pyramidal cells and granule ceils were also strongly positive of SYN,with the mean gray scale value of the hippocampal structure being 116.27?5.70.Strongly stained neurons were also found in the entorhinal area and the subiculum,with the intensity being(11.90?1.45)/mm~2.The number of SYN positive neurons and the intensity of SYN in the experimental group were higher than those in the control group(P