ABSTRACT
Objective To study the effect of NP regimen plus concurrent irradiation on cancer cell survival of human lung adenocarcinoma.Methods A549 and H1299 cells were served as the experimental objects and they were divided into irradiation group (A group),and vinorelbine tartrate injection plus cisplatin with concurrent radiotherapy group (B group).According to the different irradiation doses A group and B group were divided into A-0,A-4,A-8 group and B-0,B-4,B-8 group.MTT was performed to measure the effect of NP regimen plus concurrent irradiation on the cell proliferation,and FACS was used to detect the effect of NP regimen plus concurrent irradiation on apoptosis and P-gp expression.Results Except the cell inhibition rate of B-0 group and A-0 group had no significant difference,the cell inhibition rate of B-0,B-4 and B-8 group at 6,12,24 and 48 h were significantly lower than those of A-0,A-4 and A-8 group.The apoptosis rate of A-8 group [(45.34 ±4.77)％] was significantly higher than that of A-4 group [(20.19 ± 3.76)％] (t =7.17,P =0.006),the apoptosis rate of A-4 group was significantly higher than that of A-0 group [(4.01 ± 0.95)％] (t =7.23,P=0.019).The apoptosis rate ofB-8 group [(51.12 ± 3.37)％]was significantly higher than that of B-4 group[(36.18 ± 4.73)％] (t =3.59,P =0.037),the apoptosis rate of B-4 group was significantly higher than that of B-0 group [(14.78 ± 2.37)％] (t =9.00,P =0.003).The apoptosis rate of B-0 group [(14.78 ± 2.37)％] was significantly higher than that of A-0 group [(4.01±0.95)％] (t =7.31,P =0.018),the apoptosis rate of B-4 group was significantly higher than that of A-4 group (t =4.58,P=0.020),but there was no significant difference between B-8 group and A-8 group (t =1.71,P =0.185).The S cycle percentage of cells in B-0,B-4 and B-8 groups were significantly higher than those of A-0,A-4 and A-8 group (P ＜0.05).There were no significant differences among the P-gp fluorescence intensity of A-0,A-4 and A-8 group (P ＞ 0.05).The P-gp fluorescence intensity of B-0 group was significantly lower than that of A-0 group (t =-3.45,P =0.041),the P-tp fluorescence intensity of B-4 group was significantly lower than that of A-4 group(t =-5.85,P =0.010).The P-gp fluorescence intensity of B-8 group was significantly lower than that of A-8 group (t =-8.07,P =0.015).The P-gp fluorescence intensity of B-4 group was significantly lower than that of B-0 group (t =-8.07,P=0.015),and there was no significant difference between the mean fluorescence intensity of B-4 group and B-8 group (t =-3.56,P =0.071).Conclusion NP regimen plus concurrent irradiation could significantly inhibit the proliferation and apoptosis of human lung cancer cells,and decrease the expression of P-gp in lung cancer cells.
ABSTRACT
ObjectiveTo determine the expression of matrix metalloproteinase-2 (MMP-2),cysteine rich protein 61 (Cyr61) and CD25 in esophageal carcinoma and investigate their clinical significance.MethodThe expression of MMP-2, Cyr61 and CD25 in 68 cases of esophageal tumor tissues (esophageal carcinoma group ) and part-cancer( para-cancer group ) and 68 cases of reflux esophagitis (control group ) was detected by immunohistochemical method and the results were statistically analysed. ResultsThe positive expression rates of MMP-2,Cyr61 and CD25 were 82.35％(56/68), 73.53％(50/68) and 89.71％(61/68)respectively in esophageal carcinoma group,there were significant differences compared with those in paracancer group and control group(P ＜ 0.05) ; while there was no significantdifference between para-cancer group and control group (P ＞ 0.05 ). The positive expression rates of MMP-2, Cyr61 and CD25 in esophageal carcinoma group in different T stage, N stage and M stage all had statisticall differences (P ＜ 0.05),while there was no significant difference in different degree of differentiation (P ＞ 0.05 ). ConclusionMMP-2,Cyr61 and CD25 is highly expressed in esophageal carcinoma,there is some significance for understanding the occurrence, development and prognosis of esophageal carcinoma.
ABSTRACT
Objective To compare the curative effect of conservative treatment and surgical treatment for bronchiectasis. Methods 60 patients with bronchiectasis were randomly divided into observation group and control group,30 cases in each group,they were treated with surgery and medical therapy respectively,the clinical efficacy,length of stay, costs, remission time of symptoms and signs, changes of blood gas index and ESR, CRP,TNF-α, WBC,NEUT of the two groups after treatment were compared. Results The curative rate of observation group was significandy higher than control group, while the length of stay, remission time of symptoms and signs and hospitalization costs were significantly lower than control group, the difference was statistically significant (all P ＜0.05) ;After treatmenit,the blood gas index of both groups improved significantly than before treatment(all P ＜ 0.05), and the blood gas index of observation group improved significantly than the control group (all P ＜ 0. 05); After treatment, ESR, CRP,TNF-α,WBC and NEUT of both groups were significantly decreased compared with those before treatment(all P ＜0. 05); Between the two groups, the index value of the observation group decreased significantly (all P ＜ 0.05).Conclusion Surgical treatment for bronchiectasis was superior to conservative treatment with better curative effect,lower cost, shorter hospital stay and faster relief of symptoms and signs, it had good prospects to be applied.