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Objective To analyze the difference of salivary microbiome between the patients with functional dyspepsia and healthy controls.Methods Saliva samples were collected from the patients with functional dyspepsia and healthy control.Genomic DNA of the samples was extracted,and the high-throughput sequencing technology was used to conduct DNA sequence of 16S rRNA-V4 region.Subsequently,all the data were performed by the bioinformatic analysis.Results The salivary microbiome in the functional dyspepsia group was dominated by Proteobacteria,while Bacteroidetes was the top microbiota in the heathy control group.In the functional dyspepsia group,the Chao1 index,ACE index,Shannon index and Simpson index were 1 295,1 351,4.93 and 0.90 respectively.In the healthy control group,the above indexes were 1 001,1 351,5.28 and 0.92 respectively.The PCoA ba sically separated the microbiome composition of the two groups.Sixteen kinds of microbiota were significantly different between two groups using linear discriminant analysis effect size tool,including Bacteroidetes,Prevotella,Prevotellaceae,Neisseria,Betaproteobacteria and Proteobacteria,etc.Conclusion Saliva in the patients with functional dyspesia has characteristic microbiome composition,which is dominated by Proteobacteria,but the richness and diversity between the two groups have no difference.Neisseria is one of the significantly different bacteria between the two groups.
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Objective To discuss effect and nursing process of endoscopic biliary stent implantation for treatment of patients with malignant biliary obstruction.Methods To evaluate efficiency of the operation and to observe nursing situation of 47 cases treated by the way of endoscopic biliary stent implantation in our hospital.Results In 47 patients,31 patients used EMBE,16 patients were given ERBD,the total rate of treatment efficiency reached 100％.Positive and effective nursing measures were given to two groups during operation period,which ensured the smooth process and increase the success rate of operation.Conclusions Endoscopic biliary stent placement for malignant biliary obstruction has gradually become an effective substitution of normal operations.And active and reasonable nursing can increase the success rate of operation and decrease postoperative complications.
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Objective To investigate the requirement status and effect of relevant knowledge education in patients undergoing painless colonoscopy examination. Methods 230 cases of patients undergoing painless colonoscopy were chosen.The requirement status of relevant knowledge of painless colonoscopy examination was taken for investigation.The patients were randomly divided into the control group (90 cases) and the observation group( 140 cases).The control group was given basic nursing of painless bowel endoscopy and passively answering the questions raised by patients.The observation group was given active,systematic and designed knowledge education and psychological support for patients and their families on the basis of the control group.The knowledge level,coordination degree with examination and satisfaction degree of patients with painless colonoscopy were taken for statistics. Results The patients and their families were most concerned about security of painless colonoscopy,followed by instruction and compliance points before and after the examination,and the knowledge of anesthesia was rarely concerned about.The related knowledge of painless colonoscopy for the two groups of patients before the examination improved significantly than that after newly diagnosed,but the observation group was better than the control group.The compliance and inspection time in the observation group were significantly better than the control group.The satisfaction degree of patients and their families in the observation group were significantly higher than the control group. Conclusions The security for painless colonoscopy and instruction and compliance points before and after the examination were urgently needed by patients.Systematic knowledge education has pivotal significance in ensuring examination quality,improving satisfaction degree of patients,which is worthy of clinical application.