Molecular Pharmacognosy on Tibetan Traditional Medicine Dong-Na Duan-Chi / 中国中医药信息杂志

Objective To identify the common Tibetan herb Dong-Na Duan-Chi by molecular pharmacognosy. Methods Ethnopharmacology investigation was carried out and local herbalists were visited to observe which plants were being used as Dong-Na Duan-Chi. Then, nuclear ribosomal DNA ITS sequences were amplified and sequenced for molecular identification. Results The botanic origins of Dong-Na Duan-Chi in Tibet Autonomous Region were authenticated as two species, i.e. Veronica eriogyne H.Winkl. and Veronica ciliata Fisch.. The sequences of ITS for Veronica eriogyne and Veronica ciliata are 551 bp and 552 bp in size. The results showed that nrDNA ITS sequences could be used to discriminate V.eriogyne and V.ciliata from closely related species. Conclusion nrDNA ITS sequences can be used as the molecular markers to identify the Tibetan herb Dong-Na Duan-Chi from other species of the same genus Veronica L.

Study on Extraction of cpDNA from Endangered Medicinal Plant of Gentiana straminea Maxim . and Gentiana crassicaulis Duthie ex Burk / 世界科学技术-中医药现代化

This study was aimed to show the extraction research of cpDNA in Gentiana straminea Maxim. and G. crassicaulis Duthie ex Burk., which will increase the knowledge about the chloroplast genome sequence characteris-tics, understand its genetic diversity and improve the efficiency of breeding schemes. G. straminea Maxim. and G. crassicaulis Duthie ex Burk. were taken as study objects. The improved sucrose-gradient centrifugation and purifica-tion methods were used to obtain the cpDNA, measure the concentration, detect the OD value, and amplify the ITS2 sequence to verify the nucleus pollution. The results showed that the high quality and purity cpDNA (0.1 - 0.4 μg/10 g) was demonstrated without other pollution after ITS2 sequence amplification, which met to the subsequent effi-cient sequencing of chloroplast genomes. The purity and mass have an important influence on the accuracy and cred-ibility of the cp genome sequencing. It was concluded that this study improved the sucrose-gradient centrifugation and purification with great effect of the purity and mass of cpDNA, and guaranteed the obtaining of complete cpDNA of Gentiana.