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Objective To discuss the application value of modified Hodge test(MHT) for screening carbapenemase-producing Enterobacteriaceae.Methods The 24 Enterobacteriaceae reduced susceptibility to carbapenems were detected by MHT.At the same time,polymerase chain reaction(PCR) was used to detect carbapenemase genes of KPC,NDM,IMP,SIM and VIM.PCR products were sequenced and the results were compared with the sequences of Gen Bank database.Comprehensive analysis the application value of MHT and PCR to detect carbapenemase.Results Among these 24 strains,13 stains appeared to produce carbapenemase by MHT,5 positive strains were found to carry carbapenemase genes by PCR.By comparing with the sequences of Gen Bank database 1 strain were confirmed to KPC-2 and 4 strains were confirmed to IMP-4.We found that 4 strains of Enterobacteriaceae,detected carbapenemase by MHT and PCR at the same time.9 strains of MHT were positive,but we couldn′t detect the carbapenemase genes.1 strain of MHT was negative,but carbapenemase gene was found in the strain.Conclusion The value of MHT to screen carbapenemase-producing Enterobacteriaceae is necessary to further study.
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Objective To know the contents and changes situation of trace elements among children aged 0-6 years old in Chengdu are‐a .Methods The BH5100T atomic absorption spectrometry was adopted to detect the levels of trace elements Cu ,Zn ,Ca ,Mg ,Fe in 786 children aged 0-6 years old undergoing the physical examination in our hospital from April 2015 to November 2015 .The detection results were statistically analyzed .Results The deficiency rates of Cu ,Zn ,Ca ,Mg and Fe in Chengdu area were 0 .4% ,5 .0% ,18 .1% ,9 .5% and 8 .3% respectively ,in which the deficiency rates of Ca in children aged <1 ,1 ,2 ,3 ,≥ 4 years old were 47 .4% ,27 .5% ,12 .6% ,9 .9% and 11 .9% respectively ,indicating that the Ca deficiency rate in children < 1 years old was higher .The Ca deficiency rate had statistical differ‐ence among different age groups(P<0 .05) ,and there were no statistically significant differences in the deficiency rates of several trace ele‐ments among different age groups of male and female children .Conclusion The abnormality rate of trace elements among children aged 0-6 years old in Chengdu area is higher ,in which the Ca deficiency rate is highest ,meanwhile the Mg and Fe were lack too .The trace element content in children is closely related with the feeding habit .The 0-6 years old children in this area should pay attention to the supplement of trace elements ,especially supplement of Ca ,meanw hile breastfeeding is advocated .
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Objective:To investigate the apoptosis and changes of caspase-3,8 activity and mRNA expression induced by glycodeoxycholate(GCDC) in cultured normal human hepatocyte HL-7702 cell in vitro and to explore the possible mechanism of hepatocyte apoptosis.Methods:HL-7702 cells were cultured with GCDC,the final concentration of which were 100、150、200 and 250 ?mol/L respectively.The apoptosis ratio of HL-7702 cell was determined by AnnexinⅤ-FITC/PI double staining.Colorimetric method was used to measure caspase-3,8 activity.The expression of caspase-3,8 mRNA was determined by RT-PCR.Results:When hepatocytes were incubated with GCDC for 24 hours,the apoptotic ratio of hepatocyte,caspase-3,8 activity and mRNA expression of HL-7702 cells increased remarkably and were concentration-dependent on GCDC.Conclusion: The caspase-3,8 were concerned with the hepatocyte apoptosis induced by GCDC.The mechanism might be related to the upregulation of caspase-8 activity which subsequently transforms caspase-3 into its active form.
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Objective To investigate the apoptosis of cultured normal human hepatocyte HL-7702 cells induced by glycodeoxycholate(GCDC)and to explore its possible mechanism.Methods HL-7702 cells were incubated with various concentrations(100,150,200 and 250 ?mol/L)of GCDC.The changes of cellular morphology were observed under optical microscope.The apoptosis rate of HL-7702 was determined by Annexin V-FITC/PI double staining.The changes of HL-7702 cell intracelluar \[Ca2+\]i were determined with Fluo-3/AM load technique.The mRNA expression levels of Bcl-2/Bax in HL-7702 cells were analyzed by RT-PCR.Results Typical apoptotic morphological changes were observed after HL-7702 cells had been treated with 150 ?mol/L GCDC for 24 h;HL-7702 cells could be induced to undergo apoptosis in a concentration-dependent manner after 100,150,200,and 250 ?mol/L GCDC treatment for 24 hours.The apoptosis rates were(13.16?2.9)%,(20.3?3.0)%,(25.02?2.1)% and(45.02?3.5)%,which were markedly higher(P