ABSTRACT
Acanthopanax senticosus, a traditional herb commonly found in Northeastern Asia, has been used for treating neurodegenerative diseases. However, the molecular and cellular mechanisms of its effect on neuroinflammation have not been investigated. In the current study, quantitative proteomics approach was applied to investigate the effect of Acanthopanax senticosus extract [ASE] on nitrosative stress and inflammatory response in BV-2 microglial cells stimulated with lipopolysaccharide [LPS]. The results showed ASE inhibited LPS-induced nitric oxide [NO] production, while no significant toxicity appeared in the cells. Proteomic quantitative analyses using 2D-DIGE [Difference in Gel Electrophoresis] combined with liquid chromatography-electrospray ionization tandem mass spectrometry [LC-ESIMS/MS] revealed that 17 proteins showed significant changes responding to ASE. Furthermore, signal transduction pathways and network modulated by the ASE were predicted using Ingenuity Pathway Analysis [IPA]. These findings indicated the ability of ASE to suppress LPS-induced nitrosative stress in BV-2 cells, and may provide important insights into the molecular mechanism underlying potentially beneficial effect of Acanthopanax senticosus
ABSTRACT
Naturally existing alpha -glucosidase inhibitors from traditional herbal medicines have attracted considerable interest to treat type 2 diabetes mellitus [T2DM]. Hundreds of herbs have been reported to have the potential to inhibit alpha - glucosidase. However, most common methods to examine the inhibitors of alpha -glucosidase are usually time-consuming. In the current study, the screening of alpha -glucosidase ligands from Coptis chinensis Franch extract was undertaken by ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry [ultrafiltration LCESI-MS[n]]. Resultantly, the enzyme inhibition studies showed that Coptis chinensis Franch extract carries the strongest alpha - glucosidase inhibitory activity among the five kinds of Chinese herbal extracts. Subsequently, five compounds that could bind to alpha -glucosidase in the Coptis chinensis Franch extract were found using ultrafiltration liquid chromatography, and their structures were identified by ESI-MS[n] to be coptisine, epiberberine, jatrorrhizine, berberine, palmatine. Cumulatively, these results were anticipated to be encouraging for applying the Coptis chinensis Franch extracts as efficient anti-diabetic drug candidates