ABSTRACT
Objective To establish the quality standard for Tibetan medicine MNXT granule.Methods Inula racemosa,Tinospora sinensis were identified by TLC; Isoalantolactone and alantolactone were determined by HPLC.Results Inula racemosa,Tinospora sinensis could be identified by TLC.The concentration of isoalantolactone was linear in the range of 0.281～0.842 μg,r=0.9998,with the average recovery rate being 98.5％,RSD being1.14％.The concentration of alantolactone was linear in the range of 0.232～0.696 μg,r=0.9999,with the average recovery rate being 97.4％,RSD being 1.10％.Conclusion The method was simple,accurate,repeatable and able to control the quality of preparation effectively.
ABSTRACT
OBJECTIVE:To establish a method for the quality control of Jiuweiniuhuang pills.METHODS:TLC was applied for the quantitative identification of Bostaurus domesticus,Carthamus tinctorius and Aucklandia lappa in this prescription as well as the testing of the limit of Aristolochic acid A. RP-HPLC method was established for determination of Costunolide and Dehydrocostuslactone.RESULTS: The TLC spots were clear,well-separated,specific and free from interference of negative sample. The content of Aristolochic acid A which stood at less than 2.5 ?g?6 g-1 was up to the standard.Costunolide showed a linear relationship at the concentration range of 0.26～1.3 ?g with an average recovery of 99.8% (RSD=1.1%,n=6); and Dehydrocostuslactone showed a linear relationship at the concentration range of 0.258～1.29 ?g with an average recovery of 98.2%(RSD=1.7%,n=6).CONCLUSION: The method is suitable for the quality control of Jiuweiniuhuang pills.