ABSTRACT
This paper reports on the comparative susceptibility of A. dirus (Hainan strain) and A. stephensi (Hor strain) to the B strain of P. cynomolgi in paired feeding experi-ments. In the most susceptible infective period, the infection rate in midgut and salivary gland of the two species was over 90 %, the difference is not statistically significant.In relatively infective period, three experiments were performed, the infection rate in midgut of A. dirus was 24.4-60.4% with an average of 46.5% (53/114), and that of A. stephensi was 11.5-21.1% with an average of 14.2% (16/113). Statistically, experiment I showed no significant difference, the difference in experiment n was highly significant (P
ABSTRACT
Anopheles dirus Peyton et Harrison 1979 is an important vector of malaria in Southeast Asia and China especially in the hilly forest regions. It is an urgent problem to establish a natural mating colony of this species in a laboratory condition for malaria research. This article is to report the experience of raising A, dirus in our laboratory. The larvae were fed with a mixture of dry powdered pig liver and yeast in a ratio of 1:3. The adult mosquitoes were fed with 10% glucose solution containing 4% orange juice and 0.05% para-amino benzoic acid. Two types of cages were used as the insectary for adult mosquitoes; the large cage was 50?50?100 cm in size while the small cage 31?20?22 cm. The insectary was maintained under a temperature of 25-28℃ and a relative humidity of 80%.It was illuminated with a 30 w fluorescent light in the day time and a 15 w blue light in the night to induce mating. In the large cage, rockery and vegetations were furnished for the resting of mosquitoes.In the large cage, the mosquito colony has been propagated to the 42nd generation and the natural mating rate was stablized around 80% with a highest rate of 90.63%. In the small cage, a colony was set up by transferring the 15th generation mosquitoes from the large cage and has been propagated to the 28th generation. The natural mating rate in the small cage reached 68.96% with a highest rate of 80.36% at the 14th generation. Though it dropped slightly after the 14th generation during the summer season, it was still maintained at a satisfactory level.In addition, the optimal time and conditions to induce natural mating and to transfer mosquitoes from the large to the small cage were discussed.
ABSTRACT
Anopheles dims Peyton et Harrison, 1979 is an important malaria vector in Southeast Asia and China particularly in forested hilly zones. To establisha a laboratory mating colony for this mosquito is one of the subjects to be solved urgently in malaria research.This paper is to report our experience on breeding this mosquito. Ecological environment is created to simulate the natural conditions. The room-temperature of the insectary is 25?-28℃ and its relative humidity is around 80%.Mosquitoes are kept in cages with the si2e of 50 ? 50 ? 100cm. The larvae are fed with a mixture of de-fatted pig liver powder and yeast with a ratio of 1:3. The adults are fed with a mixture syrup containing 4% of orange juice, 10% of sucrose and other materials. The insectary is illuminated with a 3o w fluorescent light in the day time and a 15 w blue light in the night continuously to induce mating.The mating rate of the fourteenth generation reached 68% and the descendants bred can be used in research work now.
ABSTRACT
Anopheles dirus was fed with the mice of Kunming strain infected by plas-modium yoelii yoelii (By265 strain) for three or four days. Then the midgut and the salivary glands of the mosquitoes were examined. It was found that the infection rate of the malaria parasite of the midgut was 78.57% (44/56), but that of the salivary glands was only 4.88% (2/41) . After two passages of the malaria parasite through the mice and the mosquitoes, the infection rate of the salivery glands could be increased up to 13.96% (6/43).