ABSTRACT
Objective: To establish and optimize the HPLC fingerprints of Angelica sinensis medicinal material and determine the ligustilide content to improve the quality standard for Angelica sinensis and improve the quality control level of Chinese angelica medici-nal material and preparations. Methods: A method for the determination of ligustilide was optimized by HPLC. The column was eluted on an Agilent ZORBAX SB C18(250 mm×4. 6 mm, 5 μm) column with acetonitrile-water (60 ∶ 40) as the mobile phase. The flow rate was 1. 0 ml·min-1, the detection wavelength was 326 nm, and the column temperature was at 35 ℃. The HPLC fingerprints of 13 batches of Angelica sinensis from different origins and methodological investigations were established and validated to set up an HPLC fingerprinting evaluation method for Angelica sinensis. Acetonitrile-0. 1% formic acid was used as the mobile phase with gradient elu-tion. The flow rate was 1. 0 ml·min-1, the detection wavelength was 280nm, and the column temperature was at 25℃. Results: The results showed that under the above HPLC conditions, ligustilide had good linearity within the range of 0. 032 3-0. 645 5 mg·ml-1(r=0. 999 9), and the average recovery was 100. 5% (RSD=1. 61% ,n=6). The quality fraction of ligustilide in Angelica sinensis was 0. 885 6%-2. 382 2% . Through the establishment of HPLC fingerprints of Angelica sinensis, the characteristic profiles with better peak shape and degree of separation and 18 common peaks with better resolution were obtained. The similarities of the 13 batches of angelica were all between 0. 9 and 1. 0. Conclusion: According to the methodological investigation, the HPLC fingerprints and ligustilide con-tent determination method of Angelica sinensis are simple, reliable, stable and feasible.
ABSTRACT
Objective:To study the effects of various extracts of Tongkat Ali on uric acid excretion and renal function in rats with hyperuricemia. Methods:Totally 60 male Wistar rats were equally divided into the blank group,the model group,the positive control group,chloroform group,ethyl acetate group and n-butanol group. The rats were fed with yeast extract,adenine and potassium oxonate to make the model of hyperuricemia. After the animal model was made successfully,the other groups continued to give the modeling a-gent except for the blank group,and the other groups were given the corresponding medicine except for the model group after giving the modeling agent for one hour. After the model agent was given for 14 days,the levels of uric acid(UA),urea nitrogen(UN) and cre-atinine (Cr) in serum and urine and the activity of xanthine oxidase (XOD) in serum and liver were measured. Results: Compared with those in the model group,the levels of uric acid in chloroform group and n-butanol group were significantly lower than those in the model group (P<0.05),besides,the levels of uric acid and creatinine in urine in chloroform group and n-butanol group significantly increased (P<0.05). Compared with that in the model group,the XOD activity of chloroform group and n-butanol group significantly decreased (P<0.01). Compared with those in the positive control group,the levels of UA,Cr and UN in serum and urine and the ac-tivity of XOD in serum and liver in chloroform group, ethyl acetate group and n-butanol group had no significant differences (P >0.05). The levels of UA,Cr and UN in serum and urine and the activity of XOD in serum and liver in chloroform group,ethyl acetate group and n-butanol group had no significant differences (P>0.05). Conclusion: The chloroform extract and n-butanol extract in Tongkat Ali can reduce the serum level of uric acid in rats with hyperuricemia induced by adenine, yeast extract and potassium ox-onate,and the function may be related with the activity inhibition of xanthine oxidase.
ABSTRACT
Objective To investigate the relationship of STAT3 with expressions of MMP-2,MMP-9 and TIMP-1 and with epithelial-mesenchymal transition (EMT) in breast cancer cells,and to explore the clinical significances.Methods The mRNA of STAT3 and the protein expressions of pSTAT3,MMP-2,MMP-9 and TIMP-1 of breast cancer cells in 84 patients with breast cancer were determined by real-time RT-PCR technique and immunohistochemical method in paraffin-embedded specimensm respectively.Normal breast tissues adjacent to breast cancer were taken as controls.Results mRNA expression of STAT3 was significantly higher in breast cancer tissues than in controls (t=4.513,P＜ 0.001).Protein expressions of pSTAT3,MMP-2,MMP-9 and TIMP-1 were higher in breast cancer tissues than in controls (all P＜ 0.05).There were positive correlations between pSTAT3 expression and the expressions of MMP-2,MMP-9 and TIMP-1 in breast cancer tissues (all P＜0.05).The higher levels of pSTAT3 and MMP-2 were associated with poorer differentiation and more lymph node metastasis of breast cancer (both P＜0.05).The positive expression of MMP-9 was correlated with lymph node metastasis (P＜0.05),but not with histological grading (P＞0.05).The positive expression of TIMP-1 had no associations with histological grading and lymph node metastasis (both P＞0.05).There were no significant differences in the protein expressions level of pSTAT3,MMP-2,MMP-9 and TIMP-1 between different ages and different breast tumor sizes (all P＞0.05).Conclusions The increased expression of STAT3 in breast cancer cells is closely correlated with the increased expressions of MMP-2,MMP-9 and STAT3 inhibitor TIMP-1.The activation of STAT3 gene can mediate EMT by inducing the protein expressions of MMP-2,MMP-9,which promotes the invasion and metastasis of breast cancer.